Results 41 to 50 of about 9,767 (189)

Association of Type I DNA Topoisomerase with Herpes Simplex Virus

Journal of General Virology, 1985
A topoisomerase activity is associated with herpes simplex virus type 1. The enzyme was recovered from purified virions which were disrupted with 6 M-guanidine-HCl followed by renaturation of extracted proteins. Based upon the following observations, the virion activity is classified as a type I topoisomerase: (i) the linking number of a unique DNA ...
M T, Muller, C S, Bolles, D S, Parris
openaire   +2 more sources

The Effect of Dimethyl Sulfoxide on Supercoiled DNA Relaxation Catalyzed by Type I Topoisomerases [PDF]

BioMed Research International, 2015
The effects of dimethyl sulfoxide (DMSO) on supercoiled plasmid DNA relaxation catalyzed by two typical type I topoisomerases were investigated in our studies. It is shown that DMSO in a low concentration (less than 20%, v/v) can induce a dose-related enhancement of the relaxation efficiency ofEscherichia colitopoisomerase I (type IA).
Lv, Bei, Dai, Yunjia, Liu, Ju, Zhuge, Qiang, Li, Dawei   +4 more
openaire   +3 more sources

Amsacrine Derivatives Selectively Inhibit Mycobacterial Topoisomerase I (TopA), Impair M. smegmatis Growth and Disturb Chromosome Replication

Frontiers in Microbiology, 2018
Amsacrine, which inhibits eukaryotic type II topoisomerase via DNA intercalation and stabilization of the cleavable topoisomerase-DNA complex, promotes DNA damage and eventually cell death.
Marcin J. Szafran, Marta Kołodziej, Patrycja Skut, Brahmam Medapi, Agnieszka Domagała, Damian Trojanowski, Jolanta Zakrzewska-Czerwińska, Jolanta Zakrzewska-Czerwińska, Dharmarajan Sriram, Dagmara Jakimowicz, Dagmara Jakimowicz   +10 more
doaj   +1 more source

Roles of type 1A topoisomerases in genome maintenance in Escherichia coli. [PDF]

PLoS Genetics, 2014
In eukaryotes, type 1A topoisomerases (topos) act with RecQ-like helicases to maintain the stability of the genome. Despite having been the first type 1A enzymes to be discovered, much less is known about the involvement of the E.
Valentine Usongo, Marc Drolet
doaj   +1 more source

The antimicrobial activity of exogeno us anionic phospholipids against Mycobacterium tuberculosis and Escherichia coli

Тонкие химические технологии, 2016
The effect of anionic phospholipids, namely, cardiolipin, phosphatidylglycerol and phosphatidic acid, on the growth of gram-negative bacteria E. coli BL21(DE3), as well as gram-positive bacteria M. tuberculosis H37Rv was investigated in this study.
Yu. L. Mikulovich, G. M. Sorokoumova, А. А. Selishcheva, V. I. Shvets   +3 more
doaj   +1 more source

Stabilization of type I topoisomerase-DNA covalent complexes by actinomycin D. [PDF]

Proceedings of the National Academy of Sciences, 1988
The activity of the endogenous DNA topoisomerase type I (EC 5.99.1.2) can be quantified in situ by determining how efficiently the enzyme is trapped in a covalent complex with DNA upon lysis of nuclei with detergents. In this way, we can measure relative levels of topoisomerase binding to DNA at native sites in chromatin.
D K, Trask, M T, Muller
openaire   +2 more sources

Distribution bias and biochemical characterization of TOP1MT single nucleotide variants

Scientific Reports, 2017
Mitochondrial topoisomerase I (TOP1MT) is a type IB topoisomerase encoded in the nucleus of vertebrate cells. In contrast to the other five human topoisomerases, TOP1MT possesses two high frequency single nucleotide variants (SNVs), rs11544484 (V256I ...
Hongliang Zhang, Yeonee Seol, Keli Agama, Keir C. Neuman, Yves Pommier   +4 more
doaj   +1 more source

Antibacterial activity of a DNA topoisomerase I inhibitor versus fluoroquinolones in Streptococcus pneumoniae.

PLoS ONE, 2020
The DNA topoisomerase complement of Streptococcus pneumoniae is constituted by two type II enzymes (topoisomerase IV and gyrase), and a single type I enzyme (topoisomerase I).
Myriam V Valenzuela, Mirian Domenech, Patricia Mateos-Martínez, Fernando González-Camacho, Adela G de la Campa, Maria Teresa García   +5 more
doaj   +1 more source

Identification of a vaccinia virus gene encoding a type I DNA topoisomerase. [PDF]

Proceedings of the National Academy of Sciences, 1987
Vaccinia virus encapsidates a type I DNA topoisomerase (EC 5.99.1.2). The enzyme was purified from virus cores to apparent homogeneity, yielding a protein of Mr 32,000. The amino-terminal sequence of the isolated Mr 32,000 polypeptide was determined and used to map the putative structural gene for the vaccinia topoisomerase to the H7r open reading ...
S, Shuman, B, Moss
openaire   +2 more sources

Proteasome inhibitor, ixazomib prevents topoisomerase‐I degradation and reverses irinotecan resistance in colorectal cancer

Molecular Oncology, EarlyView.
Ixazomib inhibits proteasome‐mediated degradation of topoisomerase I induced by irinotecan, thereby restoring drug sensitivity and promoting tumor cell death in colorectal cancer. Irinotecan, a topoisomerase I (topoI) inhibitor, is widely used for colorectal cancer, but resistance remains a major clinical challenge.
Yuho Ebata, Koji Ando, Hirofumi Hasuda, Koshi Mimori, Elizabeth C. Unan, Siddhartha Pulukuri, Aahana Tiku, Allison Berger, Eiji Oki, Ajit Bharti, Tomoharu Yoshizumi   +10 more
wiley   +1 more source