Results 31 to 40 of about 17,370 (252)
Topoisomerases I and III inhibit R-loop formation to prevent unregulated replication in the chromosomal Ter region of Escherichia coli. [PDF]
PLoS Genetics, 2018 Type 1A topoisomerases (topos) are the only ubiquitous topos. E. coli has two type 1A topos, topo I (topA) and topo III (topB). Topo I relaxes negative supercoiling in part to inhibit R-loop formation. To grow, topA mutants acquire compensatory mutations,
Julien Brochu +4 more
doaj +1 more source
Antibiotics, 2021 Antibiotic resistance in Streptococcus pneumoniae has increased worldwide, making fluoroquinolones an alternative therapeutic option. Fluoroquinolones inhibit the type II DNA topoisomerases (topoisomerase IV and gyrase).
Jose Manuel Tirado-Vélez +5 more
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Determination of the Primary Molecular Target of 1,2,4-Triazole-Ciprofloxacin Hybrids [PDF]
, 2015 We have synthesized and examined the antibacterial activity, toxicity and affinity towards bacterial type II topoisomerases of a series of 1,2,4-triazole-ciprofloxacin hybrids.
Agata Paneth +12 more
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Structure of the human type I DNA topoisomerase gene
Journal of Biological Chemistry, 1991 We describe the molecular organization of the human gene coding for type I DNA topoisomerase. The coding sequence is split into 21 exons distributed over at least 85 kilobase pairs (kb) of human genomic DNA. The sizes of the 20 introns vary widely between 0.2 and at least 30 kb and all contain the sequence elements known to be required for pre-mRNA ...
N, Kunze +5 more
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Camptothecin, A Specific Inhibitor of Type I DNA Topoisomerase, Induces DNA Breakage at Replication Forks [PDF]
Molecular and Cellular Biology, 1988 The structure of replicating simian virus 40 minichromosomes, extracted from camptothecin-treated infected cells, was investigated by biochemical and electron microscopic methods. We found that camptothecin frequently induced breaks at replication forks close to the replicative growth points.
K, Avemann +3 more
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PLoS Genetics, 2023 The prototype enzymes of the ubiquitous type IA topoisomerases (topos) family are Escherichia coli topo I (topA) and topo III (topB). Topo I shows preference for relaxation of negative supercoiling and topo III for decatenation.
Julien Brochu +3 more
doaj +1 more source
Stabilization of type I topoisomerase-DNA covalent complexes by actinomycin D. [PDF]
Proceedings of the National Academy of Sciences, 1988 The activity of the endogenous DNA topoisomerase type I (EC 5.99.1.2) can be quantified in situ by determining how efficiently the enzyme is trapped in a covalent complex with DNA upon lysis of nuclei with detergents. In this way, we can measure relative levels of topoisomerase binding to DNA at native sites in chromatin.
D K, Trask, M T, Muller
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On the viability of
BMC Microbiology, 2012 Background Manipulations of the DNA double helix during replication, transcription and other nucleic acid processing cause a change of DNA topology, which results in torsional stress.
Stockum Anna +2 more
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Association of Type I DNA Topoisomerase with Herpes Simplex Virus
Journal of General Virology, 1985 A topoisomerase activity is associated with herpes simplex virus type 1. The enzyme was recovered from purified virions which were disrupted with 6 M-guanidine-HCl followed by renaturation of extracted proteins. Based upon the following observations, the virion activity is classified as a type I topoisomerase: (i) the linking number of a unique DNA ...
M T, Muller, C S, Bolles, D S, Parris
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Roles of type 1A topoisomerases in genome maintenance in Escherichia coli. [PDF]
PLoS Genetics, 2014 In eukaryotes, type 1A topoisomerases (topos) act with RecQ-like helicases to maintain the stability of the genome. Despite having been the first type 1A enzymes to be discovered, much less is known about the involvement of the E.
Valentine Usongo, Marc Drolet
doaj +1 more source