Results 31 to 40 of about 9,767 (189)

Mechanism and Specificity of DNA Strand Exchange Catalyzed by Vaccinia DNA Topoisomerase Type I [PDF]

Biochemistry, 2010
The type I DNA topoisomerase from vaccinia virus (vTopo) forms a reversible covalent 3'-phosphotyrosyl linkage with a single strand of duplex DNA at the preferred sequence 5'-(C/T)CCTTp downward arrowN(-1)N(-2)N(-3)-3'. The enzyme-DNA covalent adduct is recombinogenic in cells, because the nicked strand downstream of the cleavage site can dissociate ...
Mary R, Stahley, James T, Stivers
openaire   +2 more sources

Mutational Diversity in the Quinolone Resistance-Determining Regions of Type-II Topoisomerases of Salmonella Serovars

Antibiotics, 2021
Quinolone resistance in bacterial pathogens has primarily been associated with mutations in the quinolone resistance-determining regions (QRDRs) of bacterial type-II topoisomerases, which are DNA gyrase and topoisomerase IV. Depending on the position and
Aqsa Shaheen, Anam Tariq, Mazhar Iqbal, Osman Mirza, Abdul Haque, Thomas Walz, Moazur Rahman   +6 more
doaj   +1 more source

Structure of the human type I DNA topoisomerase gene

Journal of Biological Chemistry, 1991
We describe the molecular organization of the human gene coding for type I DNA topoisomerase. The coding sequence is split into 21 exons distributed over at least 85 kilobase pairs (kb) of human genomic DNA. The sizes of the 20 introns vary widely between 0.2 and at least 30 kb and all contain the sequence elements known to be required for pre-mRNA ...
N, Kunze, G C, Yang, M, Dölberg, R, Sundarp, R, Knippers, A, Richter   +5 more
openaire   +2 more sources

Purification and characterization of type I DNA topoisomerase fromLentinus edodes [PDF]

FEMS Microbiology Letters, 1986
Abstract Type I DNA topoisomerase was purified from the lower eukaryote Lentinus edodes . Like the topoisomerase I from other eukaryotic cells, the L. edodes enzyme removed both positive and negative superhelical turns. The M r of the enzyme was determined to be 71,500 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
H. Kono, N. Habuka, K. Shishido
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Topoisomerases I and III inhibit R-loop formation to prevent unregulated replication in the chromosomal Ter region of Escherichia coli. [PDF]

PLoS Genetics, 2018
Type 1A topoisomerases (topos) are the only ubiquitous topos. E. coli has two type 1A topos, topo I (topA) and topo III (topB). Topo I relaxes negative supercoiling in part to inhibit R-loop formation. To grow, topA mutants acquire compensatory mutations,
Julien Brochu, Émilie Vlachos-Breton, Sarah Sutherland, Makisha Martel, Marc Drolet   +4 more
doaj   +1 more source

Seconeolitsine, the Novel Inhibitor of DNA Topoisomerase I, Protects against Invasive Pneumococcal Disease Caused by Fluoroquinolone-Resistant Strains

Antibiotics, 2021
Antibiotic resistance in Streptococcus pneumoniae has increased worldwide, making fluoroquinolones an alternative therapeutic option. Fluoroquinolones inhibit the type II DNA topoisomerases (topoisomerase IV and gyrase).
Jose Manuel Tirado-Vélez, David Carreño, David Sevillano, Luis Alou, José Yuste, Adela G. de la Campa   +5 more
doaj   +1 more source

Camptothecin, A Specific Inhibitor of Type I DNA Topoisomerase, Induces DNA Breakage at Replication Forks [PDF]

Molecular and Cellular Biology, 1988
The structure of replicating simian virus 40 minichromosomes, extracted from camptothecin-treated infected cells, was investigated by biochemical and electron microscopic methods. We found that camptothecin frequently induced breaks at replication forks close to the replicative growth points.
K, Avemann, R, Knippers, T, Koller, J M, Sogo   +3 more
openaire   +2 more sources

Single-stranded DNA catenation mediated by human EVL and a type I topoisomerase [PDF]

Nucleic Acids Research, 2010
The human Ena/Vasp-like (EVL) protein is considered to be a bifunctional protein, involved in both actin remodeling and homologous recombination. In the present study, we found that human EVL forms heat-stable multimers of circular single-stranded DNA (ssDNA) molecules in the presence of a type I topoisomerase in vitro. An electron microscopic analysis
Takaku, Motoki, Takahashi, Daisuke, Machida, Shinichi, Ueno, Hiroyuki, Hosoya, Noriko, Ikawa, Shukuko, Miyagawa, Kiyoshi, Shibata, Takehiko, Kurumizaka, Hitoshi   +8 more
openaire   +2 more sources

On the viability of Escherichia coli cells lacking DNA topoisomerase I

BMC Microbiology, 2012
Background Manipulations of the DNA double helix during replication, transcription and other nucleic acid processing cause a change of DNA topology, which results in torsional stress.
Stockum Anna, Lloyd Robert G, Rudolph Christian J   +2 more
doaj   +1 more source

Characterization of a pathway of genomic instability induced by R-loops and its regulation by topoisomerases in E. coli.

PLoS Genetics, 2023
The prototype enzymes of the ubiquitous type IA topoisomerases (topos) family are Escherichia coli topo I (topA) and topo III (topB). Topo I shows preference for relaxation of negative supercoiling and topo III for decatenation.
Julien Brochu, Émilie Vlachos-Breton, Dina Irsenco, Marc Drolet   +3 more
doaj   +1 more source