DNA-Topology Simplification by Topoisomerases [PDF]
Molecules, 2021 The topological properties of DNA molecules, supercoiling, knotting, and catenation, are intimately connected with essential biological processes, such as gene expression, replication, recombination, and chromosome segregation. Non-trivial DNA topologies
Andreas Hanke +2 more
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Cell-free DNA topology depends on its subcellular and cellular origins in cancer [PDF]
JCI Insight, 2022 Cancer cells release large quantities of cell-free DNA (cfDNA) into the surrounding tissue and circulation. As cfDNA is a common source of biomarkers for liquid biopsy and has been implicated as a functional mediator for intercellular communication ...
Ethan Z. Malkin +8 more
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Controlling the topology of mammalian mitochondrial DNA
Open Biology, 2021 The genome of mitochondria, called mtDNA, is a small circular DNA molecule present at thousands of copies per human cell. MtDNA is packaged into nucleoprotein complexes called nucleoids, and the density of mtDNA packaging affects mitochondrial gene ...
Katja E. Menger +3 more
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Topoisomerase 2β and DNA topology during B cell development [PDF]
Frontiers in Immunology, 2022 Topoisomerase 2β (TOP2B) introduces transient double strand breaks in the DNA helix to remove supercoiling structures and unwind entangled DNA strains. Advances in genomic technologies have enabled the discovery of novel functions for TOP2B in processes ...
Olivier Papapietro +4 more
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Fluorescently labeled circular DNA molecules for DNA topology and topoisomerases. [PDF]
Sci Rep, 2016 DNA topology plays essential roles in several fundamental biological processes, such as DNA replication, recombination, and transcription. Typically agarose gel electrophoresis is employed to study DNA topology.
Gu M +5 more
europepmc +2 more sources
Innovative Tools for DNA Topology Probing in Human Cells Reveal a Build-Up of Positive Supercoils Following Replication Stress at Telomeres and at the FRA3B Fragile Site [PDF]
CellsLinear unconstrained DNA cannot harbor supercoils since these supercoils can diffuse and be eliminated by free rotation of the DNA strands at the end of the molecule.
Claire Ghilain +5 more
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Journal of Extracellular Vesicles, 2019 Extracellular vesicles have the capacity to transfer lipids, proteins, and nucleic acids between cells, thereby influencing the recipient cell’s phenotype.
Elisa Lázaro-Ibáñez +7 more
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DNA Topology and the Initiation of Virus DNA Packaging. [PDF]
PLoS ONE, 2016 During progeny assembly, viruses selectively package virion genomes from a nucleic acid pool that includes host nucleic acids. For large dsDNA viruses, including tailed bacteriophages and herpesviruses, immature viral DNA is recognized and translocated ...
Choon Seok Oh +8 more
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Pamoic acid and carbenoxolone specifically inhibit CRISPR/Cas9 in bacteria, mammalian cells, and mice in a DNA topology-specific manner [PDF]
Genome BiologyBackground Regulation of the target DNA cleavage activity of CRISPR/Cas has naturally evolved in a few bacteria or bacteriophages but is lacking in higher species.
Yuxuan Zhang +5 more
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Editorial: Chromosome architecture and DNA topology in prokaryotes [PDF]
Frontiers in Microbiology, 2023 Tamara Basta +2 more
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