Results 251 to 260 of about 2,766,615 (303)

Topologically‐Interlocked Minicircles as Probes of DNA Topology and DNA‐Protein Interactions

Chemistry – A European Journal, 2022
AbstractDNA minicircles exist in biological contexts, such as kinetoplast DNA, and are promising components for creating functional nanodevices. They have been used to mimic the topological features of nucleosomal DNA and to probe DNA‐protein interactions such as HIV‐1 and PFV integrases, and DNA gyrase.
Arivazhagan Rajendran, Kirankumar Krishnamurthy, Seojeong Park, Eiji Nakata, Youngjoo Kwon, Takashi Morii   +5 more
openaire   +3 more sources

DNA Topology

Choice Reviews Online, 2005
Abstract DNA as the genetic material is a topic of intense interest in the 21st century, with the genomes of many organisms having been sequenced. The double helix structure discovered by Watson and Crick 50 years ago is now very familiar and its importance for DNA function is widely appreciated.
Andrew D Bates, Anthony Maxwell
openaire   +2 more sources

Epigenetics, fragmentomics, and topology of cell-free DNA in liquid biopsies

Science, 2021
Enhancing liquid biopsies Liquid biopsies, analyses of cell-free DNA that circulates in the blood, can be used in prenatal testing, oncology, and to monitor organ transplant recipients. Lo et al. review the nongenetic information that can be gleaned from
Y. Lo, D. S. Han, P. Jiang, R. Chiu
semanticscholar   +1 more source

Energetics of twisted DNA topologies

Biophysical Journal, 2021
Our goal is to review the main theoretical models used to calculate free energy changes associated with common, torsion-induced conformational changes in DNA and provide the resulting equations hoping to facilitate quantitative analysis of both in vitro and in vivo studies.
Wenxuan, Xu, David, Dunlap, Laura, Finzi
openaire   +2 more sources

Small DNA circles as probes of DNA topology.

Biochemical Society Transactions, 2013
Small DNA circles can occur in Nature, for example as protein-constrained loops, and can be synthesized by a number of methods. Such small circles provide tractable systems for the study of the structure, thermodynamics and molecular dynamics of closed-circular DNA.
A. Bates, A. Noy, M. M. Piperakis, S. Harris, A. Maxwell   +4 more
semanticscholar   +3 more sources

Introduction to DNA Topology

Discrete and Topological Models in Molecular Biology, 2014
In this expository chapter we give an elementary introduction to DNA and to proteins that can knot and link circular DNA, with a special focus on recombination. We also describe the Ernst and Sumners tangle model of the action of proteins on circular DNA.
I. Darcy, S. Levene, R. Scharein
semanticscholar   +2 more sources

Chemical Modulation of DNA Replication along G-Quadruplex Based on Topology-Dependent Ligand Binding.

Journal of the American Chemical Society, 2021
Ligands that bind to and stabilize guanine-quadruplex (G4) structures to regulate DNA replication have therapeutic potential for cancer and neurodegenerative diseases. Because there are several G4 topologies, ligands that bind to their specific types may
Shuntaro Takahashi, Anita Kotar, H. Tateishi-Karimata, Sudipta Bhowmik, Zi-Fu Wang, Ta-Chau Chang, Shinobu Sato, S. Takenaka, J. Plavec, N. Sugimoto   +9 more
semanticscholar   +1 more source

Interlocked DNA topologies for nanotechnology

Current Opinion in Biotechnology, 2017
Interlocked molecular architectures are well known in supramolecular chemistry and are widely used for various applications like sensors, molecular machines and logic gates. The use of DNA for constructing these interlocked structures has increased significantly within the current decade.
Valero Moreno, Julian, Lohmann, Finn, Famulok, Michael   +2 more
openaire   +4 more sources

Expression of transfected DNA depends on DNA topology

Cell, 1986
Supercoiled DNAs, especially those containing enhancers, yield markedly higher levels of expression than linearized DNA when transfected into CV-1 cells or L cells. Different templates, linear or supercoiled, enhancer-containing or not, saturate for expression at 2 micrograms DNA per dish, suggesting that one role for enhancers and supercoiling is to ...
H, Weintraub, P F, Cheng, K, Conrad
openaire   +2 more sources

Topology-dependent DNA binding

2023
ABSTRACTDNA stores our genetic information and is ubiquitous in biological and biotechnological applications, where it interacts with binding partners ranging from small molecules to large macromolecular complexes. Binding is modulated by mechanical strains in the molecule and, in turn, can change the local DNA structure.
Pauline J. Kolbeck, Miloš Tišma, Brian T. Analikwu, Willem Vanderlinden, Cees Dekker, Jan Lipfert   +5 more
openaire   +1 more source