Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Aeration-Driven Dynamics and Compositional Transformation of Dissolved Organic Carbon in Surface Water Containing Microplastics and Microalgae. [PDF]
Cahyonugroho OH +6 more
europepmc +1 more source
O filme-ensaio | El filme-ensayo | The essay film | Le film-essai
O filme-ensaio | El filme-ensayo | The essay film | Le film-essaiO filme-ensaio | El filme-ensayo | The essay film | Le film-essaiO filme-ensaio | El filme-ensayo | The essay film | Le film-essaiO filme-ensaio | El filme-ensayo | The essay film | Le film-
On-Line, DOC
core
Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão +3 more
wiley +1 more source
Differences in the Subcortical White Matter Integrity Based on the Disorder of Consciousness at the Chronic Stage of Diffuse Axonal Injury: A Tract-Based Spatial Statistics Study. [PDF]
Jang SH, Cho MJ, Byun DH.
europepmc +1 more source
Voices of VISTA: Interview with Doc Severinsen
Willis Conover interviews trumpeter and bandleader Doc Severinsen for Voices of VISTA program #112.
Conover, Willis
core
Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard +12 more
wiley +1 more source
Atterrissage réussi pour Lab&doc à Nantes !
La doc du crenau a eu le plaisir d'accueillir la commission recherche du réseau francophone ArchiRès les 30 novembre et 1er décembre. Décidée en juillet lors du séminaire de Strasbourg, cette réunion déployée sur deux jours comportait un volet formation ...
Laurence Bizien
core
Yeast Gcn2 retains activity following humanization of its auto‐phosphorylation region
Using Saccharomyces cerevisiae as a model to study Gcn2 activation and regulation is limited by the lack of antibodies detecting phosphorylated Gcn2. To overcome this, we engineered Gcn2‐HsC, a yeast Gcn2 variant recognizable by commercial anti‐human phospho‐GCN2 antibodies.
Reuben A. Anderson +2 more
wiley +1 more source

