Results 91 to 100 of about 685,461 (196)

Dot enzyme-linked immunosorbent assay (dot-ELISA) for schistosomiasis diagnosis using dacron as solid-phase

open access: yes, 2017
Dacron and nitrocellulose were evaluated as matrices for the dot enzyme linked immunosorbent assay (dot-ELISA) for schistosomiasis and compared to indirect immunofluorescence (IMF).
Silva, Joanne D'arc Bezerra da   +3 more
core  

A Machine Vision‐Guided Microphysiological Platform With Automated Microfluidics Enables Longitudinal Biomarker Monitoring and Emulation of Translationally Relevant Exposure Scenarios

open access: yesAdvanced Science, EarlyView.
A pneumatically actuated multi‐tissue microphysiological system is integrated with AI‐based machine vision and automatic sampling and replenishment systems. The platform allows for the emulation of translationally relevant long‐term pharmacokinetic exposure scenarios for multiple weeks while enabling longitudinal monitoring of response biomarkers ...
Jibbe Keulen   +15 more
wiley   +1 more source

A Pathology‐Instructed Theranostic Platform with Mechanoadaptive and ROS‐Powered Nanobreathing Functions for Precision Myocardial Repair

open access: yesAdvanced Science, EarlyView.
We developed a pH responsive theranostic serum albumin hydrogel named BST, functionalized with MRI and CT probes and loaded with mitochondria targeted CAT‐SOD nanogels, that rapidly self repairs and mechanically stabilizes the infarcted myocardium, releases therapeutic nanogels and albumin under hypoxia induced acidosis to clear mitochondrial ROS ...
Zheng Luo   +7 more
wiley   +1 more source

THBS1+ Macrophages Exacerbate Modic Changes via SDC4‐Dependent Activation of NLRP3 Inflammasome

open access: yesAdvanced Science, EarlyView.
The illustration of THBS1+ macrophages exacerbate MCs via SDC4‐dependent activation of the NLRP3 inflammasome. THBS1+ macrophage subpopulation was identified in MCs through single‐cell RNA sequencing. C. acnes and its metabolites activate THBS1 expression in macrophages via the TLR signaling pathway.
Xiangxi Kong   +16 more
wiley   +1 more source

Femtosecond‐Laser Nanocavitation Regenerates SERS‐Active Plasmonic Nanogaps for Longitudinal Molecular Sensing at Biointerfaces

open access: yesAdvanced Science, EarlyView.
A regenerative molecular sensing platform that co‐localizes surface‐enhanced Raman spectroscopy (SERS) sensing and nanocavitation‐based actuation. Femtosecond‐laser triggered nanocavitation produces thermomechanical forces to locally regenerate SERS‐active nanogaps in protein‐rich biofluids while preserving optical performance.
Aditya Garg   +8 more
wiley   +1 more source

Engineering Human Donor Derived Germinal Center‐Like Organoids (GCLOs) for Studying Immune Response to Vaccination

open access: yesAdvanced Science, EarlyView.
We bioengineered a new approach methodology of generating Germinal Center Organoids that self‐organize from human blood‐derived immune cells. These immune organoids reproduce key features of humoral immunity, IgG production, and plasmablast emergence.
Bhumi Suthar   +4 more
wiley   +1 more source

Pulmoner tüberküloz tanısında Dot Immunobinding Assay ve ELISA yöntemleriyle antijen aramanın yeri

open access: yes, 1995
ÖZET: Pulmoner tüberkülozda erken tanı koymak amacıyla yapılan bu Çalışmada, enzyme-linked immunosorbent assay (ELISA) ve dot- immunobinding assay (Dot-lba) yöntemleriyle balgamda mikobakteriyel antijenler araştırıldı.
TEKİN KARSLIGİL
core  

Biodegradable Acoustic Targeting for Ultrasound‐Supported Gene Therapy (BATUS) in Glioblastoma

open access: yesAdvanced Science, EarlyView.
ABSTRACT Glioblastoma (GBM) remains one of the most challenging brain malignancies due to the restrictive nature of the blood–brain barrier (BBB), which severely limits effective drug and gene delivery. To overcome this, we introduce Biodegradable Acoustic Targeting for Ultrasound‐Supported Gene Therapy (BATUS), a modular platform that enables safe ...
Gulsah Erel‐Akbaba   +25 more
wiley   +1 more source

In-House IgM Dot-Blot Assay for Serodiagnosis of Human Leptospirosis: Development, Standardisation, and Performance Evaluation

open access: yesMicroorganisms
Laboratory confirmation of human leptospirosis relies on serological tests, with the microscopic agglutination test (MAT) as the reference. However, due to its complexity, there is a need for a simpler and more accessible diagnostic method.
Roberta Morozetti Blanco   +4 more
doaj   +1 more source

A Hydrogen Sulfide–Releasing Dynamic Hydrogel Modulates Coordinated Neurovascular, Immune, and Angiogenic Responses for Scar‐Suppressed Diabetic Wound Repair

open access: yesAdvanced Science, EarlyView.
ROS‐Responsive H2S Hydrogel (HAPPF) Coordinated Regeneration for High‐Quality Diabetic Wound Repair.A self‐regulating dynamic hydrogel is developed to deliver a fluorogenic H2S donor in response to excessive ROS. Released H2S acts as a master regulator to resolve chronic inflammation (M2 polarization), restore VEGF‐driven angiogenesis, and rebalance ...
Xuyang Ning   +7 more
wiley   +1 more source

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