Results 71 to 80 of about 60,295 (214)

Highly Sensitive and Specific Monoclonal Antibody-Based Serological Methods for Rice Ragged Stunt Virus Detection in Rice Plants and Rice Brown Planthopper Vectors

Journal of Integrative Agriculture, 2014
Rice ragged stunt virus (RRSV) is a serious rice disease in Asia, causing serious yield losses on rice. The capsid protein (CP) gene of the major outer capsid protein of RRSV was expressed in Escherichia coli BL21 (DE3) using the pMAL-C2X expression ...
Huan LIU, Xi-jiao SONG, Yue-qun NI, Li-na LU, Xue-ping ZHOU, Jian-xiang WU   +5 more
doaj   +1 more source

Erratum: Pixel-Based Machine Learning and Image Reconstitution for Dot-ELISA Pathogen Diagnosis in Biological Samples. [PDF]

Front Microbiol, 2021
Frontiers Production Office.
europepmc   +1 more source

A Multidimensional Engineering Strategy Reprograms Microglia via Targeted and Sustained‐Release Extracellular Vesicles for Spinal Cord Injury Repair

Advanced Science, EarlyView.
A multifunctional EV‐based nanoplatform (Ang‐TEVs@Gel) was engineered via preconditioning, surface targeting, and ROS‐responsive hydrogel encapsulation to reprogram microglia. This system restored autophagy via miR‐664a‐3p/PIK3CA axis, cleared myelin debris, resolved neuroinflammation, and promoted axon remyelination, ultimately achieving robust motor ...
Wu Xiong, Minhao Liu, Mingming Zheng, Jizhou Jia, Ziyan Zhu, Guang Kong, Jie Liu, Juan Wang, Wenbo Li, Qingyuan Wang, Qian Zhu, Peiran Chan, Tao Qin, Jiayang Wu, Yongjie Zhang, Chunming Tang, Cong Li, Jin Fan   +17 more
wiley   +1 more source

Development, optimization, and validation of an in-house Dot-ELISA rapid test based on SAG1 and GRA7 proteins for serological detection of Toxoplasma gondii infections

Infection and Drug Resistance, 2019
Aref Teimouri,1,2 Mohammad Hossein Modarressi,3 Saeedeh Shojaee,1 Mehdi Mohebali,1,4 Mostafa Rezaian,1 Hossein Keshavarz1,41Department of Medical Parasitology and Mycology, Tehran University of Medical Sciences, Tehran, Iran; 2Students Scientific ...
Teimouri A, Modarressi MH, Shojaee S, Mohebali M, Rezaian M, Keshavarz H   +5 more
doaj  

Development of nanobody-based flow-through dot ELISA and lateral-flow immunoassay for rapid detection of 3-phenoxybenzoic acid. [PDF]

Anal Methods, 2021
Zhang C, Wu X, Li D, Hu J, Wan D, Zhang Z, Hammock BD.   +6 more
europepmc   +1 more source

MetaboSense: An Integrated Quantum Dot‐Mediated Aptamer Assay and Modular Microfluidic Platform for Continuous In‐Line Monitoring of Lung Metabolism During Ex Vivo Perfusion

Advanced Science, EarlyView.
MetaboSense is a first‐of‐its‐kind microfluidic platform enabling continuous, in‐line monitoring of glucose and lactate during ex vivo lung perfusion. Combining a quantum dot–mediated aptamer assay with mixing, depletion, and optofluidic detection modules, it achieves 60–s temporal resolution and shows strong agreement with standard blood gas analyzers,
Sanjana Srikant, Pouyan Keshavarz Motamed, Thomas Borrillo, Lielle Ronen, Gabriel Siebiger, Guillermo Garza, Hesam Abouali, Md Fahim Al Fattah, Ines Jaber, Dayan Ban, Marcelo Cypel, Mingyao Liu, Shaf Keshavjee, Andrew T. Sage, Mahla Poudineh   +14 more
wiley   +1 more source

Dot enzyme-linked immunosorbent assay (dot-ELISA) for schistosomiasis diagnosis using dacron as solid-phase

Revista da Sociedade Brasileira de Medicina Tropical, 1999
Dacron and nitrocellulose were evaluated as matrices for the dot enzyme linked immunosorbent assay (dot-ELISA) for schistosomiasis and compared to indirect immunofluorescence (IMF).
Silvia Maria Lucena Montenegro, Joanne D'arc Bezerra da Silva, Maria Edileuza Felinto de Brito, Luiz Bezerra de Carvalho Junior   +3 more
doaj  

Versatile Co‐Engineering of Immunogenic Cell Death‐Primed Dead Tumor Cell Bodies With Tumor‐Activated MnO2 for cGAS‐STING‐Potentiated Personalized Immunotherapy

Advanced Science, EarlyView.
DTBEI@CS@MnO2, fabricated via modular electrostatic co‐engineering and photothermal/photodynamic immunogenic cell death, specifically responds to the acidic and GSH‐rich tumor microenvironment. This triggers the release of DTBEI that provides tumor antigens, DNA, and DAMPs, as well as the generation of Mn2+, thereby synergistically potentiating cGAS ...
Bolun Xu, Xuan Lu, Yuquan Wang, Haihua Ji, Ying Zhang, Yiqun Wan, Hao Wan   +6 more
wiley   +1 more source

Diagnosis of Human Leptospirosis: Comparison of Microscopic Agglutination Test with Recombinant LigA/B Antigen-Based In-House IgM Dot ELISA Dipstick Test and Latex Agglutination Test Using Bayesian Latent Class Model and MAT as Gold Standard. [PDF]

Diagnostics (Basel), 2022
Behera SK, Sabarinath T, Ganesh B, Mishra PKK, Niloofa R, Senthilkumar K, Verma MR, Hota A, Chandrasekar S, Deneke Y, Kumar A, Nagarajan M, Das D, Khatua S, Sahu R, Ali SA.   +15 more
europepmc   +1 more source

Chemical Bond‐Orchestrated Energy Depletion to Augment Lung Cancer Chemo‐Immunotherapy

Advanced Science, EarlyView.
This study grafted dichloroacetic acid (DCA) into perylenediimide (PDI) skeleton via ionic or covalent bond to create novel metabolic agent PDIC‐AC and PDIC‐NAC, explicitly elaborated the underlying energy metabolism and therapeutic mechanism, and developed a promising strategy for energy metabolism‐targeting chemo‐immunotherapy by chemical bond ...
Xuejie Zhao, Yuting Liu, Xiao Huang, Liwen Zhao, Chunli Li, Yongwei Huang   +5 more
wiley   +1 more source