Results 81 to 90 of about 214,168 (304)
Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae
FEBS Open Bio, EarlyView.Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj +3 more
wiley +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
, 2008 This article demonstrates that double object constructions in Archaic Chinese display both patterns of direct and indirect object marking (DO+IO) and primary and secondary object marking (PO+SO).
Phua, Chiew Pheng
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The Effects of Referential Factors in Mojeno Trinitario Derived and Non-Derived Ditransitive Verbs
Linguistic Discovery, 2012 This paper investigates the effects of referential factors (more specifically the person hierarchy) in non-derived and derived three-participant constructions of Mojeno Trinitario, an Arawak language of Bolivia. The basic effect of referential properties
Françoise Rose
doaj +1 more source
Case assignment in double object constructions [PDF]
Revista Alicantina de Estudios Ingleses, 1997 This paper analyzes how case assignment takes place in double object constructions in English. It has been claimed in the literature that case could be either structural or inherent. I will provide some evidence showing that this distinction is problematic under certain circumstances. In this sense, I will suggest three different possibilities to unify
openaire +3 more sources
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
More than meets the eye: Toward a reassessment of Old English double accusatives
ExELL (Explorations in English Language and Linguistics)This article offers a corpus-based update of the Accusative-Accusative construction as part of a much-needed reanalysis of Old English double-object complementation.
Juan G. Vázquez González
doaj +1 more source
FEBS Open Bio, EarlyView.The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer +7 more
wiley +1 more source
Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs
FEBS Open Bio, EarlyView.Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner +7 more
wiley +1 more source
Larson\u27s (1988) Analysis of the Double Object Construction Revisited
, 2011 1.研究目的 2.Larson(1988)で扱っている現象 3.Larson(1988)の分析 4.Larson(1988)の問題点 5.今後の研究に向けてThis paper presents a critical review of the analysis of the double object construction proposed in Larson (1988).
楊, 郭, Yang, Guo
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