Results 31 to 40 of about 213,326 (261)

Combined DECS Analysis and Next-Generation Sequencing Enable Efficient Detection of Novel Plant RNA Viruses

Viruses, 2016
The presence of high molecular weight double-stranded RNA (dsRNA) within plant cells is an indicator of infection with RNA viruses as these possess genomic or replicative dsRNA.
Hironobu Yanagisawa, Reiko Tomita, Koji Katsu, Takuya Uehara, Go Atsumi, Chika Tateda, Kappei Kobayashi, Ken-Taro Sekine   +7 more
doaj   +1 more source

A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones

BMC Biotechnology, 2013
Background Recombineering is a genetic engineering tool that enables facile modification of large episomal clones, e.g. BACs, fosmids. We have previously adapted this technology to generate, directly from fosmid-based genomic clones, fusion gene reporter
Hirani Nisha, Westenberg Marcel, Gami Minaxi S, Davis Paul, Hope Ian A, Dolphin Colin T   +5 more
doaj   +1 more source

Targeted mutagenesis with sequence‐specific nucleases for accelerated improvement of polyploid crops: Progress, challenges, and prospects

The Plant Genome, 2023
Many of the world's most important crops are polyploid. The presence of more than two sets of chromosomes within their nuclei and frequently aberrant reproductive biology in polyploids present obstacles to conventional breeding.
David May, Katalin Paldi, Fredy Altpeter
doaj   +1 more source

On statistical convergence of double sequences of closed sets

Filomat, 2016
In this paper, we introduce the concepts of statistical inner and statistical outer limits for double sequences of closed sets and give some formulas for finding these limits. Also, we give the Kuratowski statistical convergence of double sequences of sets by means of the statistical inner and statistical outer limits of a double sequence ...
Sever, Yurdal, Talo, Özer
openaire   +4 more sources

A Strategy to Setup Codominant Microsatellite Analysis for High-Resolution-Melting-Curve-Analysis (HRM)

BMC Genetics, 2008
Background High resolution melting curve analysis (HRM) is a technique that measures exactly the decreasing fluorescence of intercalating dye in the process of dissociation of double stranded DNA.
Novak Johannes, Lukas Brigitte, Mader Eduard   +2 more
doaj   +1 more source

Compression after impact behavior of flat and tapered Single-Double carbon composite specimens

Composites Part C: Open Access
This research investigates the damage tolerance with compression after impact (CAI) testing procedure of five sets of specimens, that were manufactured using the card-sliding technique with non-crimp fabrics (NCFs) and adopting a Single-Double (SD ...
Alessandro Vescovini, Carina Xiaochen Li, Bo Cheng Jin, Andrea Manes   +3 more
doaj   +1 more source

A ddRAD Based Linkage Map of the Cultivated Strawberry, Fragaria xananassa. [PDF]

PLoS ONE, 2015
The cultivated strawberry (Fragaria ×ananassa Duch.) is an allo-octoploid considered difficult to disentangle genetically due to its four relatively similar sub-genomic chromosome sets.
Jahn Davik, Daniel James Sargent, May Bente Brurberg, Sigbjørn Lien, Matthew Kent, Muath Alsheikh   +5 more
doaj   +1 more source

Adjunctive Therapeutic Plasma Exchange in Refractory Adult‐Onset Still's Disease Complicated by Secondary Macrophage Activation Syndrome: A Single‐Center Experience

Therapeutic Apheresis and Dialysis, EarlyView.
ABSTRACT Introduction Adult‐onset Still's disease (AOSD) complicated by macrophage activation syndrome (MAS) carries substantial mortality. The role of therapeutic plasma exchange (TPE) remains uncertain. Methods We retrospectively analyzed patients with AOSD‐MAS treated with TPE at a single‐center.
Masataka Ueda, Tomohiro Saito, Masahide Mizobuchi, Tomoki Shimomura, Saki Kawamori, Mitsuru Kawanishi, Natsuko Hattori, Jo Sugiyama, Satoshi Tsubata, Hanami Horiguchi, Kazuki Abe, Nao Iida, Yuki Kajio, Nao Oguro, Ryo Yanai, Hirokazu Honda   +15 more
wiley   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Multi-objective decoupling control strategy for low voltage ride through under asymmetrical faults

电力工程技术
For the traditional low voltage ride through control under asymmetrical faults, due to the limited control degrees of freedom, the grid-connected inverter control suffers from the problem of not being able to simultaneously realize the output current ...
LIU Qinyi, ZHONG Qidi, WANG Shiwen, LU Yajun, SUN Jinping, LIU Fei   +5 more
doaj   +1 more source