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Colchicine Alternatives for Chromosome Doubling in Maize Haploids for Doubled‐Haploid Production
Crop Science, 2016In vivo production of doubled‐haploid (DH) lines in maize (Zea mays L.) routinely involves artificial chromosome doubling by colchicine treatment of seedlings. Because colchicine is a hazardous chemical, replacing it by less‐toxic alternatives would be highly desirable.
Albrecht E. Melchinger +3 more
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2022
Doubled haploid (DH) technology reduces the time required to obtain homozygous genotypes and accelerates plant breeding among other advantages. It is established in major crop species such as wheat, barley, maize, and canola. DH lines can be produced by both in vitro and in vivo methods and the latter is focused here.
Siddique I. Aboobucker +6 more
openaire +3 more sources
Doubled haploid (DH) technology reduces the time required to obtain homozygous genotypes and accelerates plant breeding among other advantages. It is established in major crop species such as wheat, barley, maize, and canola. DH lines can be produced by both in vitro and in vivo methods and the latter is focused here.
Siddique I. Aboobucker +6 more
openaire +3 more sources
Haploids and doubled haploids in Citrus ssp.
2003Citrus breeding is based either on conventional (hybridization, selection, mutation) or biotechnological methods, the latter employing in vitro tissue culture, regeneration from protoplasts, somatic hybridization, in vitro mutant selection, genetic transformation and haploid/doubled haploid production. All cultivated forms of Citrus spp.
openaire +1 more source
2015
Climate changes, a rapid pathogen evolution, and market change, put breeders under constant pressure to deliver new and better varieties in a shorter time while simultaneously reducing the costs of variety development. Substantial progress has been generated in triticale doubled haploid (DH) technologies during the last decade that widens their ...
Maria Wędzony +5 more
openaire +1 more source
Climate changes, a rapid pathogen evolution, and market change, put breeders under constant pressure to deliver new and better varieties in a shorter time while simultaneously reducing the costs of variety development. Substantial progress has been generated in triticale doubled haploid (DH) technologies during the last decade that widens their ...
Maria Wędzony +5 more
openaire +1 more source
2003
There are many factors influencing the deployment of doubled haploidy in breeding. Strategies for exploiting doubled haploids (DHs) vary within and among species and are dependent upon available technologies. Theoretical benefits and working protocols are not however sufficient to justify DH deployment in breeding.
W. T. B. Thomas +2 more
openaire +1 more source
There are many factors influencing the deployment of doubled haploidy in breeding. Strategies for exploiting doubled haploids (DHs) vary within and among species and are dependent upon available technologies. Theoretical benefits and working protocols are not however sufficient to justify DH deployment in breeding.
W. T. B. Thomas +2 more
openaire +1 more source
Molecular Karyotyping on Populus simonii × P. nigra and the Derived Doubled Haploid
International Journal of Molecular Sciences, 2021Quanwen Dou, Wang Sui, Bo Liu
exaly
Doubled Haploid Production in Higher Plants
2017The use of doubled haploidy is a powerful tool in crop improvement programs and basic research. A haploid embryo or plant has the gametic chromosome number (n). When haploid cells are doubled, the resulting embryo/plant will have the somatic chromosome number (2n) and be 100% homozygous.
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