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Multispectral and thermal infrared data, visual scores for severity of common rust symptoms, and genotypic single nucleotide polymorphism data of three F2-derived biparental doubled-haploid maize populations. [PDF]
Data BriefLoladze A +9 more
europepmc +1 more source
Anther culture for haploid and doubled haploid production [PDF]
Plant Cell, Tissue and Organ Culture, 2010 Haploids are plants with a gametophytic chromosome number and doubled haploids are haploids that have undergone chromosome duplication. The production of haploids and doubled haploids (DHs) through gametic embryogenesis allows a single-step development of complete homozygous lines from heterozygous parents, shortening the time required to produce ...
Maria Antonietta Germanà
exaly +2 more sources
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Analysis of Ploidy in Haploids and Doubled Haploids
2021Determination of the ploidy level is an essential step when trying to produce doubled haploids (DHs) in any species. Each species and method used to produce DHs has its own frequency of DH production, which means that the rest of plants produced stay haploid.
Ochatt, Sergio J, Seguí-Simarro, Jose
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Species with Haploid or Doubled Haploid Protocols
2021In this chapter, we present a list of species (and few interspecific hybrids) where haploids and/or doubled haploids have been published, including the method by which they were obtained and the corresponding references. This list is an update of the compilation work of Maluszynski et al.
Jose M, Seguí-Simarro +3 more
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Developments and prospects for doubled haploid wheat
Biotechnology Advances, 2022Doubled haploid production is a valuable biotechnology that can accelerate the breeding of new wheat varieties by several years through the one-step creation of 100% homozygous plants. The technology also plays important role in studying the genetic control of traits in wheat, in marker-assisted selection, in genomics and in genetic engineering.
Nikolai Borisjuk +2 more
exaly +4 more sources
Production of Haploids and Doubled Haploids in Maize
2012The in vivo haploid induction approach offers several advantages compared to the in vitro induction approach and recurrent self-pollination. It is currently the method of choice for inbred line development in many commercial maize breeding programs. Here, we describe the in vivo approach for generation of maternal doubled haploids (DHs).
Vanessa, Prigge, Albrecht E, Melchinger
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Doubled Haploid Technology: Generation of Doubled Haploid Maize Lines Using Haploid Inducers
Cold Spring Harbor ProtocolsDoubled haploid (DH) technology allows for the development of completely homozygous lines from heterozygous plants in only two generations. This approach has been widely adopted in maize breeding programs, as it expedites the generation of inbred lines compared to traditional methods. The DH approach is based on the use of maize genotypes that have the
Vencke K. Grüning +2 more
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Colchicine Alternatives for Chromosome Doubling in Maize Haploids for Doubled‐Haploid Production
Crop Science, 2016In vivo production of doubled‐haploid (DH) lines in maize (Zea mays L.) routinely involves artificial chromosome doubling by colchicine treatment of seedlings. Because colchicine is a hazardous chemical, replacing it by less‐toxic alternatives would be highly desirable.
Albrecht E. Melchinger +3 more
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Haploids and doubled haploids in Citrus ssp.
2003Citrus breeding is based either on conventional (hybridization, selection, mutation) or biotechnological methods, the latter employing in vitro tissue culture, regeneration from protoplasts, somatic hybridization, in vitro mutant selection, genetic transformation and haploid/doubled haploid production. All cultivated forms of Citrus spp.
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2015
Climate changes, a rapid pathogen evolution, and market change, put breeders under constant pressure to deliver new and better varieties in a shorter time while simultaneously reducing the costs of variety development. Substantial progress has been generated in triticale doubled haploid (DH) technologies during the last decade that widens their ...
Maria Wędzony +5 more
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Climate changes, a rapid pathogen evolution, and market change, put breeders under constant pressure to deliver new and better varieties in a shorter time while simultaneously reducing the costs of variety development. Substantial progress has been generated in triticale doubled haploid (DH) technologies during the last decade that widens their ...
Maria Wędzony +5 more
openaire +1 more source