Results 31 to 40 of about 20,502 (251)

Application of dried blood spot sample pooling strategies for Plasmodium 18S rRNA biomarker testing to facilitate identification of infected persons in large-scale epidemiological studies

Malaria Journal, 2021
Background Plasmodium 18S rRNA is a sensitive biomarker for detecting Plasmodium infection in human blood. Dried blood spots (DBS) are a practical sample type for malaria field studies to collect, store, and transport large quantities of blood samples ...
Ming Chang, Selena Johnston, Annette M. Seilie, Dianna Hergott, Sean C. Murphy   +4 more
doaj   +1 more source

Next-Generation Molecular Testing of Newborn Dried Blood Spots for Cystic Fibrosis [PDF]

The Journal of Molecular Diagnostics, 2016
Newborn screening for cystic fibrosis enables early detection and management of this debilitating genetic disease. Implementing comprehensive CFTR analysis using Sanger sequencing as a component of confirmatory testing of all screen-positive newborns has remained impractical due to relatively lengthy turnaround times and high cost.
Lefterova, Martina I., Shen, Peidong, Odegaard, Justin I., Fung, Eula, Chiang, Tsoyu, Peng, Gang, Davis, Ronald W., Wang, Wenyi, Kharrazi, Martin, Schrijver, Iris, Scharfe, Curt   +10 more
openaire   +2 more sources

HIV-1 RNA testing of pooled dried blood spots is feasible to diagnose acute HIV infection in resource limited settings

Southern African Journal of Infectious Diseases, 2018
Objectives:Rapid human immunodeficiency virus (HIV) antibody tests, routinely used for diagnosis in adults and older children in resource-limited settings (RLS), do not detect early HIV infections prior to seroconversion or when antibody levels are still
Wentzel Dowling, Kirsten Veldsman, Mary G. Katusiime, Jean Maritz, Peter Bock, Sue-Ann Meehan, Marije Van Schalkwyk, Mark F. Cotton, Wolfgang Preiser, Gert U. Van Zyl   +9 more
doaj   +1 more source

Utility of Dried Blood Spots for the Diagnosis of Congenital Cytomegaloviruses within the First 21 Days of Life in a Single Center

International Journal of Neonatal Screening, 2023
In this retrospective study, we aimed to evaluate the performance of dried-blood-spot (DBS) testing as a diagnostic method for the congenital cytomegalovirus (cCMV).
Ana Del Valle Penella, Jerry Miller, Ryan Rochat, Gail Demmler-Harrison   +3 more
doaj   +1 more source

Fully Automated Forensic Routine Dried Blood Spot Screening for Workplace Testing [PDF]

Journal of Analytical Toxicology, 2018
In this study, we describe the transfer of a new and fully automated workflow for the cost-effective drug screening of large populations based on the dried blood spot (DBS) technology. The method was installed at a routine poison control center and applied for DBS and dried urine spot (DUS) samples. A fast method focusing on the high-interest drugs and
Stefan, Gaugler, Maha K, Al-Mazroua, Sahar Y, Issa, Jana, Rykl, Matthias, Grill, Asem, Qanair, Vicente L, Cebolla   +6 more
openaire   +2 more sources

Postal methods for monitoring HbA1c in diabetes mellitus: a protocol for systematic review

BJGP Open, 2022
Background: Worldwide there are an estimated 463 million people with diabetes. In the UK people with diabetes are offered annual review, which includes monitoring of haemoglobin A1c (HbA1c).
Jack Colley, Hajira Dambha-Miller, Beth Stuart, Jazz Bartholomew, Hermione Price   +4 more
doaj   +1 more source

A rapid method for DNA Isolation from blood, dried blood spots and rapid diagnosis test

Journal of Vector Borne Diseases, 2023
Background & objectives: Malaria is a parasitic disease spread by Plasmodium parasite. Microscopy, lateral flow devices such as the Rapid Diagnostic Test (RDT), molecular methods such as Polymerase Chain Reaction (PCR), isothermal methods such as Loop-mediated isothermal amplification (LAMP), and other diagnostic methods ...
Supriya Sharma, Aparajita Sen, Naseem Ahmed, Deendayal Swarnkar, Himmat Singh   +4 more
openaire   +3 more sources

Newborn Genetic Screening—Still a Role for Sanger Sequencing in the Era of NGS

International Journal of Neonatal Screening, 2023
In the Norwegian newborn screening (NBS) program, genetic testing has been implemented as a second or third tier method for the majority of NBS disorders, significantly increasing positive predictive value (PPV).
Silje Hogner, Emma Lundman, Janne Strand, Mari Eknes Ytre-Arne, Trine Tangeraas, Asbjørg Stray-Pedersen   +5 more
doaj   +1 more source

Feasibility of a dried blood spot strategy for serological screening and surveillance to monitor elimination of Human African Trypanosomiasis in the Democratic Republic of the Congo.

PLoS Neglected Tropical Diseases, 2021
In recent years, the number of reported Human African Trypanosomiasis (HAT) cases caused by Trypanosoma brucei (T.b.) gambiense has been markedly declining, and the goal of 'elimination as a public health problem' is within reach. For the next stage, i.e.
Raquel Inocencio da Luz, Delphin Mavinga Phanzu, Oscar N'lemvo Kiabanzawoko, Eric Miaka, Paul Verlé, Anja De Weggheleire, Philippe Büscher, Epco Hasker, Marleen Boelaert   +8 more
doaj   +1 more source

Evaluation of an HIV recent infection testing algorithm with serological assays among men who have sex with men in Mexico

Journal of Infection and Public Health, 2020
Background: Human immunodeficiency virus (HIV) incidence should be calculated in cross-sectional studies using recent infection testing algorithms (RITA) that consider clinical variables and serological test results such as enzyme-linked immunosorbent ...
Eli F. Karatzas-Delgado, Verónica Ruiz-González, Santa García-Cisneros, María L. Olamendi-Portugal, Antonia Herrera-Ortiz, Hugo López-Gatell, Andrea González-Rodríguez, Miguel A. Sánchez-Alemán   +7 more
doaj   +1 more source