Results 51 to 60 of about 1,821,977 (231)

Pyrosequencing dried blood spots reveals differences in HIV drug resistance between treatment naïve and experienced patients. [PDF]

PLoS ONE, 2013
Dried blood spots (DBS) are an alternative specimen collection format for HIV-1 genotyping. DBS produce HIV genotyping results that are robust and equivalent to plasma when using conventional sequencing methods.
Hezhao Ji, Yang Li, Binhua Liang, Richard Pilon, Paul MacPherson, Michèle Bergeron, John Kim, Morag Graham, Gary Van Domselaar, Paul Sandstrom, James Brooks   +10 more
doaj   +1 more source

Tumour‐proximal deep serum proteomics identifies biomarkers associated with ovarian cancer

Molecular Oncology, EarlyView.
New biomarkers are needed for early ovarian cancer detection. Ovarian (tumour‐proximal) and peripheral vein serum from healthy women and patients with benign or malignant tumours underwent nanoparticle‐enriched deep proteomics. Ovarian serum yielded more protein identifications and revealed tumour‐reflective signatures.
Darren Lau, Daniel Pincher, Clifford Young, Nicholas Chappell, Noor A. Lokman, Parul Mittal, Leigh Donnellan, Martin K. Oehler, Doug A. Brooks, Peter Hoffmann   +9 more
wiley   +1 more source

HIV RNA measurement in dried blood spots of HIV-infected patients in Thailand using Abbott m2000 system.

PLoS ONE, 2020
World Health Organization recommends using dried blood spots (DBS) for HIV RNA viral load (VL) measurement whenever plasma processing is not convenient or feasible. DBS collected from 80 treatment-naïve HIV-infected patients presenting in three hospitals
Woottichai Khamduang, Ampika Kaewbundit, Amonrat Duangmano, Sayamon Hongjaisee, Virat Klinbuayaem, Guttiga Halue, Apichat Chutanunta, Wasna Sirirungsi, Gonzague Jourdain, Nicole Ngo-Giang-Huong   +9 more
doaj   +1 more source

The plasma separation card as a novel solution for enhancing central laboratory capability for HIV-1 viral load monitoring in limited-access settings.

PLOS Global Public Health, 2023
Measurement of HIV-1 viral load (VL) is essential for monitoring antiretroviral treatment (ART) efficacy. The preferred specimen type for VL is plasma, but in remote settings where collection and preservation of plasma many not be possible, dried blood ...
Charles Kiyaga, Caroline Makoha, Ivan Nkugwa, Christopher Okiira, Richard Okwir, Sirak Zenebe Gebreab, Patricia Rodriguez-Ventosa Suarez, Benjamin LaBrot, Ana Carrasco Durán   +8 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Dried Matrix Spots for the Determination of Opiates and Opioids: Methodological Advances and Applications

Molecules
Dried matrix spot (DMS) techniques have gained increasing attention in bioanalytical and forensic toxicology for the detection of opiates and opioids, offering minimally invasive sampling, enhanced sample stability, and simplified storage and transport ...
Luana M. Rosendo, Rita Gonçalves, Rodrigo Martins, Vitória Castro, Tiago Rosado, Mário Barroso, Eugenia Gallardo   +6 more
doaj   +1 more source

Measurement and Comparison of Organic Compound Concentrations in Plasma, Whole Blood and Dried Blood Spot Samples

Frontiers in Genetics, 2016
The preferred sampling medium for measuring human exposures of persistent organic compounds (POPs) is blood, and relevant sample types include whole blood, plasma, and dried blood spots (DBS).
Stuart A Batterman, Sergei eChernyak, Feng-Chiao eSu   +2 more
doaj   +1 more source

Alinity m, a Random-Access System, for Hepatitis B Virus DNA Quantification in Plasma and Whole Blood Collected on Dried Blood Spots

mSphere, 2022
The International Liver Association recommends the use of accurate and sensitive molecular methods for determination of hepatitis B virus (HBV) DNA levels in plasma or serum of chronic HBsAg carriers. The level of HBV replication represents the strongest
Valérie Ortonne, Quentin Lucas, Olivia Garrigou, Alexandre Soulier, Dominique Challine, Jean-Michel Pawlotsky, Vincent Leroy, Stéphane Chevaliez   +7 more
doaj   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

Comparison of phenylalanine and free carnitine concentrations in dried blood spots, plasma and dried plasma spots using tandem mass spectrometry

, 2020
U svrhu provođenja novorođenačkog probira u uzorcima suhe kapi krvi određuje se profil aminokiselina i acilkarnitina. Prednost suhe kapi krvi i suhe kapi plazme u odnosu na uzorak plazme/seruma je manji volumen uzorkovanja, stabilnost analita na sobnoj ...
Šarčević, Željka
core   +1 more source