Results 61 to 70 of about 1,821,977 (231)

Effects of IGFBP4 deficiency on human preadipocyte proliferation and differentiation through the IGF1R/AKT pathway

FEBS Open Bio, EarlyView.
IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Yujia Guo, Sandy Richter, Christoph Bach, Wieland Kiess, Anna Kirstein, Diana Le Duc, Antje Garten   +6 more
wiley   +1 more source

Utility of the Cobas^® Plasma Separation Card as a Sample Collection Device for Serological and Virological Diagnosis of Hepatitis C Virus Infection

Diagnostics, 2021
Diagnosis and clinical management of people infected with hepatitis C virus (HCV) relies on results from a combination of serological and virological tests.
Fernando Velásquez-Orozco, Ariadna Rando-Segura, Joan Martínez-Camprecios, Paula Salmeron, Adrián Najarro-Centeno, Àngels Esteban, Josep Quer, María Buti, Tomás Pumarola-Suñe, Francisco Rodríguez-Frías   +9 more
doaj   +1 more source

Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA

FEBS Open Bio, EarlyView.
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley   +1 more source

Determination of mycophenolic acid and its phenyl glucuronide in human plasma, ultrafiltrate, blood, DBS and dried plasma spots

, 2010
Background: Analysis of mycophenolic acid (MPA), the active form of the immunosuppressive drug mycophenolate mofetil, and its glucuronide metabolite MPAG is required for therapeutic monitoring and postmarketing clinical studies.
Almudena Gajate-Perez, Franz Bucheli, Roswitha Hartenbach, Katja Heinig   +3 more
core   +1 more source

In situ-generated volumetric dried plasma spots for the analysis of edaravone and metabolites in animal models

Journal of Pharmaceutical and Biomedical Analysis Open
In this study, for the first time rat plasma microsampling was carried out by means of in situ-generated volumetric dried plasma spot (vDPS) technology and applied to the determination of the neuroprotective agent edaravone and its sulphate and ...
Michele Protti, Roberta Di Lecce, Jiri Adamec, Luca G. Regazzoni, Valeria Valsecchi, Claudia Volpi, Roberto Mandrioli, Laura Mercolini   +7 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Plasma level of 5-CH₃-H₄folate in 30 μl dried plasma spots after uptake of 400 μg 5-CH₃-H₄folate.

, 2015
Plasma level of 5-CH3-H4folate in 30 μl dried plasma spots after uptake of 400 μg 5-CH3-H4folate.
Markus Kopp (831010), Michael Rychlik (447033)   +1 more
core   +1 more source

HIV diversity and drug resistance from plasma and non‐plasma analytes in a large treatment programme in western Kenya

Journal of the International AIDS Society, 2014
Introduction Antiretroviral resistance leads to treatment failure and resistance transmission. Resistance data in western Kenya are limited. Collection of non‐plasma analytes may provide additional resistance information.
Rami Kantor, Allison DeLong, Maya Balamane, Leeann Schreier, Robert M Lloyd Jr, Wilfred Injera, Lydia Kamle, Fidelis Mambo, Sarah Muyonga, David Katzenstein, Joseph Hogan, Nathan Buziba, Lameck Diero   +12 more
doaj   +1 more source

Plasma EV Proteomics Identifies ECM Remodeling and Inflammatory Proteins LUM and C7 as Candidate Biomarkers in FSHD

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Facioscapulohumeral muscular dystrophy (FSHD) is one of the most debilitating and common muscular dystrophies. Despite its severity, no approved therapy exists for FSHD patients. However, several therapeutic candidates are currently under development, and some have recently entered clinical trials, marking the need for reliable ...
Mustafa Bilal Bayazit, Chiranth K. Nagaraj, Jackson S. Newell, Kim Truc Nguyen, Xilal Y. Rima, Jacob Doon‐Ralls, Eduardo Reátegui, Jeffrey M. Statland, Rabi Tawil, Kevin M. Flanigan, Scott Q. Harper, Nizar Y. Saad   +11 more
wiley   +1 more source

Detection of IL28B SNP DNA from buccal epithelial cells, small amounts of serum, and dried blood spots.

PLoS ONE, 2012
Background & aimsPoint mutations in the coding region of the interleukin 28 gene (rs12979860) have recently been identified for predicting the outcome of treatment of hepatitis C virus infection.
Philippe Halfon, Denis Ouzan, Hacène Khiri, Guillaume Pénaranda, Paul Castellani, Valerie Oulès, Asma Kahloun, Nolwenn Amrani, Lise Fanteria, Agnès Martineau, Lou Naldi, Marc Bourlière   +11 more
doaj   +1 more source