Results 121 to 130 of about 322,402 (318)

Numerical modelling of sequential droplet impingements

open access: yes, 2008
During the process of thermal spray coating, molten powders are sprayed and deposited on substrates to generate protective coatings. It is essential to have a clear understanding of the physics of droplet impingement on the surface of substrates for ...
Gu, Sai, Kamnis, S., Lu, T.J., Chen, C.
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

open access: yesFEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake   +4 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Important advances in the knowledge of lipid droplets in the last six decades

open access: yes, 2019
Lipid droplets are highly dynamic cytoplasmic (and nuclear) cell organelles composed of a core of neutral lipids (triglycerides (TG) and cholesterol ester (CE)) surrounded by a monolayer of phospholipids with embedded proteins.
Catala, Angel
core  

Electrowetting of liquid marbles [PDF]

open access: yes, 2006
Electrowetting of water drops on structured superhydrophobic surfaces are known to cause an irreversible change from a slippy (Cassie-Baxter) to a sticky (Wenzel) regime.
Elliott, SJ   +9 more
core   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

open access: yesFEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke   +4 more
wiley   +1 more source

Characterization of ultrahydrophobic hierarchical surfaces fabricated using a single-step fabrication methodology

open access: yes, 2011
Hydrophobic surfaces with microscale roughness can be rendered ultrahydrophobic by the addition of sub-micron-scale roughness. A simple yet highly effective concept of fabricating hierarchical structured surfaces using a single-step deep reactive ion ...
S V Garimella   +5 more
core   +1 more source

The skills required for transition to university and study in biological sciences: A student perspective

open access: yesFEBS Open Bio, EarlyView.
Bioscience students were asked for their opinions on the value and teaching of skills. 204 responded that teamwork, time management and study skills are necessary to reach University, that scientific writing, research, laboratory and presentation skills are taught effectively during their studies, while other skills are gained inherently through study ...
Janella Borrell, Susan Crennell
wiley   +1 more source

COST P21 "The physics of droplets"

open access: yes, 2006
During the last five years, microfluidic technology has emerged. The applications are very fruitful in many domains: biotechnology, food science, reprographics, microelectronics and nanoscience, cleaning, cooling, tribology etc.
GUIDO, STEFANO, SIMEONE, MARINO
core  

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