Exploring Strategies for Protein Trapping in Drosophila [PDF]
AbstractThe use of fluorescent protein tags has had a huge impact on cell biological studies in virtually every experimental system. Incorporation of coding sequence for fluorescent proteins such as green fluorescent protein (GFP) into genes at their endogenous chromosomal position is especially useful for generating GFP-fusion proteins that provide ...
Ana T. Quiñones-Coello+13 more
openaire +3 more sources
Cleavage of the apoptosis inhibitor DIAP1 by the apical caspase DRONC in both normal and apoptotic drosophila cells [PDF]
In Drosophila S2 cells, the apical caspase DRONC undergoes a low level of spontaneous autoprocessing. Unintended apoptosis is prevented by the inhibitor of apoptosis DIAP1, which targets the processed form of DRONC for degradation through its E3 ...
Clem, Rollie J.+2 more
core +1 more source
Drosophila's contribution to stem cell research [v1; ref status: indexed, http://f1000r.es/5h7]
The discovery of Drosophila stem cells with striking similarities to mammalian stem cells has brought new hope for stem cell research. A recent development in Drosophila stem cell research is bringing wider opportunities for contemporary stem cell ...
Gyanesh Singh
doaj +1 more source
Comprehensive analysis of the chromatin landscape in Drosophila melanogaster. [PDF]
Chromatin is composed of DNA and a variety of modified histones and non-histone proteins, which have an impact on cell differentiation, gene regulation and other key cellular processes.
Alekseyenko, Artyom A+29 more
core +4 more sources
Organizing activity of wingless protein in Drosophila
The adult appendages of Drosophila are formed from imaginal discs, sheets of epithelial cells that proliferate during larval development and differentiate during metamorphosis. wingless (wg, DWnt-1) protein, a putative signaling molecule, is expressed only in prospective ventral cells in each of the leg discs.
Struhl, G, Basler, K
openaire +4 more sources
Drosophila's contribution to stem cell research [version 2; referees: 2 approved]
The discovery of Drosophila stem cells with striking similarities to mammalian stem cells has brought new hope for stem cell research. Recent developments in Drosophila stem cell research is bringing wider opportunities for contemporary stem cell ...
Gyanesh Singh
doaj +1 more source
Identification of microtubule-associated proteins in the centrosome, spindle, and kinetochore of the early Drosophila embryo. [PDF]
We have developed affinity chromatography methods for the isolation of microtubule-associated proteins (MAPs) from soluble cytoplasmic extracts and have used them to analyze the cytoskeleton of the early Drosophila embryo.
Alberts, BM, Field, CM, Kellogg, DR
core
The K+‐Cl− cotransporters (KCCs) facilitate the symport of ions across the plasma membrane. They participate in physiological processes including neuronal regulation. Here, we characterized KCCs from Drosophila and Hydra vulgaris. Comparative analyses of transporters provide insights into the mechanism of KCC ion transport, regulation, and evolution ...
Satoshi Fudo+4 more
wiley +1 more source
Sonic Hedgehog Is a Member of the Hh/DD-Peptidase Family That Spans the Eukaryotic and Bacterial Domains of Life. [PDF]
Sonic Hedgehog (Shh) coordinates Zn2+ in a manner that resembles that of peptidases. The ability of Shh to undergo autoproteolytic processing is impaired in mutants that affect the Zn2+ coordination, while mutating residues essential for catalytic ...
Roelink, Henk
core +3 more sources
The developmental proteome of Drosophila melanogaster
Drosophila melanogaster is a widely used genetic model organism in developmental biology. While this model organism has been intensively studied at the RNA level, a comprehensive proteomic study covering the complete life cycle is still missing. Here, we
Nuria Casas-Vila+9 more
semanticscholar +1 more source