Results 241 to 250 of about 3,507,735 (293)

Rapid fluorescent assay for screening drugs on Leishmania amastigotes

Journal of Microbiological Methods, 2008
A rapid fluorescent viability assay employing alamarBlue was optimized for use with Leishmania axenic amastigotes, the stage of the parasite responsible for disease pathology. The activity of two protein kinase inhibitors, Staurosporine and H-89, as well as Amphotericin B, on promastigotes and amastigotes of Leishmania donovani and Leishmania tropica ...
Orly, Shimony, Charles L, Jaffe
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New Colorimetric Cytotoxicity Assay for Anticancer-Drug Screening

JNCI Journal of the National Cancer Institute, 1990
We have developed a rapid, sensitive, and inexpensive method for measuring the cellular protein content of adherent and suspension cultures in 96-well microtiter plates. The method is suitable for ordinary laboratory purposes and for very large-scale applications, such as the National Cancer Institute's disease-oriented in vitro anticancer-drug ...
P, Skehan, R, Storeng, D, Scudiero, A, Monks, J, McMahon, D, Vistica, J T, Warren, H, Bokesch, S, Kenney, M R, Boyd   +9 more
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The evaluation of an immunoperoxidase assay applicable in antiviral drug screening

Biologicals, 2019
Bovine viral diarrhea virus (BVDV) fall into cytopathic (CP) and noncytopathic (NCP) biotypes, based on their ability to kill cultured cells. NCP-BVDV can not be titrated by conventional means as used for CP-BVDV, which has impeded the identification of antiviral drugs targeting NCP-BVDV virus strains.
Liqian, Zhu, Liai, Huang, Aiping, Wang, Qingmei, Li, Junqing, Guo, Li, Wang, Gaiping, Zhang   +6 more
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A cell-based drug screening assay on a centrifugal platform

Biofabrication
Abstract Drug screening is an indispensable procedure in drug development and pharmaceutical research. For cell-based drug testing, cells were treated with compounds at different concentrations, and their responses were measured to assess the compounds’ effects on cellular behavior.
Chia-Tse Shih, Huan-Jun Guo, Chih-Hsin Shih, Yi-Chen Ethan Li   +3 more
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Baculoviruses and Mammalian Cell‐based Assays for Drug Screening

2006
Publisher Summary A few years ago, a new use for recombinant baculoviruses was revealed with the appearance of two publications demonstrating the delivery of recombinant gene expression cassettes containing reporter genes under control of mammalian cell-active promoters to mammalian cells primarily of liver origin.
J Patrick, Condreay, Robert S, Ames, Namir J, Hassan, Thomas A, Kost, Raymond V, Merrihew, Danuta E, Mossakowska, David J, Pountney, Michael A, Romanos   +7 more
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Preclinical in vitro screening assays for drug-like properties

Drug Discovery Today: Technologies, 2005
Besides pharmacological effects, ADMET drug properties, namely, absorption, disposition, metabolism, elimination and toxicity, are important drug properties critical for clinical success. In vitro human-based experimental system used in combination with in vivo animal systems, using animal species relevant to humans, represent the best approach to ...
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A novel drug screening assay for papillomavirus specific antiviral activity

Antiviral Research, 1998
Discovery and development of human papillomavirus (HPV) specific antiviral agents have been hampered by the lack of an in vitro assay permissive to HPV replication. An experimental assay system for monitoring HPV-11 DNA replication has been optimized for use as a papillomavirus antiviral drug screening tool.
P R, Clark, M L, Roberts, L M, Cowsert
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Development of a Yeast Bio-Assay to Screen Anti-Mitochondrial Drugs

Current Drug Discovery Technologies, 2009
Previous studies show that acetylsalicylic acid (aspirin) at low concentrations affects yeast sexual structure development in a similar fashion than oxygen depletion. This is ascribed to its anti-mitochondrial action. In this study, we report the same for other anti-inflammatory (i.e.
Johan L F, Kock, Chantel W, Swart, Desmond M, Ncango, Johan L F, Kock, Ingrid A, Munnik, Marleen M J, Maartens, Carolina H, Pohl, Pieter W J, van Wyk   +7 more
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Safety screening in early drug discovery: An optimized assay panel

Journal of Pharmacological and Toxicological Methods, 2019
Several factors contribute to the development failure of novel pharmaceuticals, one of the most important being adverse effects in pre-clinical and clinical studies. Early identification of off-target compound activity can reduce safety-related attrition in development.
Stefanie, Bendels, Caterina, Bissantz, Bernhard, Fasching, Grégori, Gerebtzoff, Wolfgang, Guba, Manfred, Kansy, Jacques, Migeon, Susanne, Mohr, Jens-Uwe, Peters, Fabien, Tillier, René, Wyler, Christian, Lerner, Christian, Kramer, Hans, Richter, Sonia, Roberts   +14 more
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Cell-Based Assays and Animal Models for GPCR Drug Screening

2015
The family of G protein-coupled receptors (GPCRs) remains a central focus of basic pharmacology and drug discovery efforts. Convenient methods to assess the efficacy of potentially therapeutic reagents for GPCRs are strongly required for high-throughput screening (HTS) assay.
Hideo, Takakura, Mitsuru, Hattori, Miho, Tanaka, Takeaki, Ozawa   +3 more
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