Discovery of novel cardiac troponin activators using fluorescence polarization-based high throughput screening assays [PDF]
Scientific Reports, 2023 The large unmet demand for new heart failure therapeutics is widely acknowledged. Over the last decades the contractile myofilaments themselves have emerged as an attractive target for the development of new therapeutics for both systolic and diastolic ...
Priyanka Parijat +6 more
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High-yield synthesis and purification of recombinant human GABA transaminase for high-throughput screening assays [PDF]
Journal of Enzyme Inhibition and Medicinal Chemistry, 2021 Many studies have focussed on modulating the activity of γ-aminobutyric acid transaminase (GABA-T), a GABA-catabolizing enzyme, for treating neurological diseases, such as epilepsy and drug addiction. Nevertheless, human GABA-T synthesis and purification
Mingu Gordon Park +5 more
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Mining Chemical Activity Status from High-Throughput Screening Assays. [PDF]
PLoS ONE, 2015 High-throughput screening (HTS) experiments provide a valuable resource that reports biological activity of numerous chemical compounds relative to their molecular targets.
Othman Soufan +6 more
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Characterizing chlorotriazine effects in cancer-relevant high-throughput screening assays [PDF]
Frontiers in ToxicologyIntroductionHigh-throughput screening (HTS) in vitro testing can be a powerful tool for evaluating chemicals across an abundance of mechanistic, targeted assay systems.
Agnes L. Karmaus, Alex Charlton
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Stress-Based High-Throughput Screening Assays to Identify Inhibitors of Cell Envelope Biogenesis [PDF]
Antibiotics, 2020 The structural integrity of the Gram-negative cell envelope is guarded by several stress responses, such as the σE, Cpx and Rcs systems. Here, we report on assays that monitor these responses in E.
Maurice Steenhuis +3 more
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Integrating AUROC and SSMD for quality control in high-throughput screening assays [PDF]
SLAS DiscoveryHigh-throughput screening (HTS) assays are pivotal in modern biomedical research, particularly in drug discovery and functional genomics. Ensuring the quality and reliability of HTS data is critical, especially when dealing with the small sample sizes ...
Xiaohua Douglas Zhang
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New High-Throughput Screening Assays for Biocatalysis
CHIMIA, 2001 High-throughput screening for catalysis is a critical technology in all experiments aimed at modifying or creating enzymes by directed evolution, as well as for biodiversity mining for new catalysts.
Jean-Louis Reymond
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Bioactivity Signatures of Drugs vs. Environmental Chemicals Revealed by Tox21 High-Throughput Screening Assays [PDF]
Frontiers in Big Data, 2019 Humans are exposed to tens of thousands of chemicals over the course of a lifetime, yet there remains inadequate data on the potential harmful effects of these substances on human health.
Deborah K. Ngan +6 more
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Analysis of high-throughput screening assays using cluster enrichment. [PDF]
Stat Med, 2012 In this paper, we describe the implementation and evaluation of a cluster‐based enrichment strategy to call hits from a high‐throughput screen using a typical cell‐based assay of 160,000 chemical compounds. Our focus is on statistical properties of the prospective design choices throughout the analysis, including how to choose the number of clusters ...
Pu M +7 more
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Spectrophotometric Enzyme Assays for High-Throughput Screening
Food Technology and Biotechnology, 2004 This paper reviews high-throughput screening enzyme assays developed in our laboratory over the last ten years. These enzyme assays were initially developed for the purpose of discovering catalytic antibodies by screening cell culture supernatants, but ...
Jean-Louis Reymond
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