Results 1 to 10 of about 1,974,930 (309)

Protocol for the colocalization of yeast peroxisomal membrane proteins and their binding partners using stimulated emission depletion microscopy

STAR Protocols
Summary: Peroxisomes are highly dynamic organelles that play crucial roles in cellular metabolism. Here, we present a protocol to investigate peroxisomal membrane proteins and their binding partners in the yeast Hansenula polymorpha using stimulated ...
Frank N. Mol, Eline M.F. de Lange, Ida J. van der Klei, Rifka Vlijm   +3 more
doaj   +1 more source

Protocol to study monocyte transmigration across primary human liver endothelial cells under physiological shear flow conditions in vitro

STAR Protocols
Summary: Modeling immune cell recruitment by liver endothelial cells in vitro is important to better understand the pathology of chronic inflammatory liver diseases and cancers.
Alex L. Wilkinson, Megan E. Bannister, Ayla O’Keeffe, Chris J. Weston, Patricia F. Lalor, Shishir Shetty, Daniel A. Patten   +6 more
doaj   +1 more source

Profound effect of profiling platform and normalization strategy on detection of differentially expressed microRNAs [PDF]

, 2012
Adequate normalization minimizes the effects of systematic technical variations and is a prerequisite for getting meaningful biological changes. However, there is inconsistency about miRNA normalization performances and recommendations.
Kaiser, Sebastian, Meyer, Swanhild U., Pfaffl, Michael W., Thirion, Christian, Wagner, Carola   +4 more
core   +1 more source

Protocol for live neuron imaging analysis of basal surface fraction and dynamic availability of the dopamine transporter using DAT-pHluorin

STAR Protocols
Summary: Surface availability of the dopamine (DA) transporter (DAT) critically influences DA transmission. Here, we present a protocol that describes the preparation of mouse ventral midbrain neurons, the expression of a new optical sensor, DAT-pHluorin,
Elnaz Khezerlou, Jacqueline Saenz, Sanjana Surya Prakash, Ping-Yue Pan   +3 more
doaj   +1 more source

An Empirical-Mathematical Approach for Calibration and Fitting Cell-Electrode Electrical Models in Bioimpedance Tests [PDF]

, 2018
This paper proposes a new yet efficient method allowing a significant improvement in the on-line analysis of biological cell growing and evolution.
Daza Navarro, María Paula, Huertas Sánchez, Gloria, Maldonado-Jacobi, Andrés, Martín Rubio, María Esther, Olmo Fernández, Alberto, Pérez García, Pablo, Rueda Rueda, Adoración (Coordinador), Serrano Viseas, Juan Alfonso, Sánchez Aguayo, Inmaculada (Coordinador), Yúfera García, Alberto   +9 more
core  

Protocol for establishing primary murine colonic fibroblast culture with induction of loxP/loxP deletion using transducible TAT-Cre recombinase

STAR Protocols
Summary: Conditional mutagenesis using site-specific recombinases is widely used in transgenic mouse models; however, its efficiency in vitro is limited by poor tamoxifen activity.
Christina McAninch, Allison Hahn, Steven McAninch, Irina V. Pinchuk, Marina Chulkina   +4 more
doaj   +1 more source

Non-coding RNAs participate in the regulatory network of CLDN4 via ceRNA mediated miRNA evasion [PDF]

, 2017
Thousands of genes have been well demonstrated to play important roles in cancer progression. As genes do not function in isolation, they can be grouped into “networks” based on their interactions.
Chen, Xiao-wan, Gao, Peng, Miao, Zhi-feng, Shi, Jin-xin, Song, Yong-xi, Sun, Jing-xu, Wang, Zhen-ning, Wu, Zhong-hua, Yang, Yu-chong, Zhao, Jun-hua   +9 more
core   +2 more sources

Antibody-antigen interactions: What is the required time to equilibrium? [PDF]

, 2010
The use of antibodies is widespread in many areas including in-vivo and in-vitro diagnostics, quantitative analysis in research laboratories and as therapeutic substances.
Hanna Bjö, Karl Andersson, Magnus Malmqvist   +2 more
core   +1 more source

Protocol for click labeling of HIV-1 envelope on amber-free virions prepared using genetic code expansion

STAR Protocols
Summary: Site-specific fluorescent labeling of HIV-1 proteins enables direct visualization of their dynamics and trafficking. Here, we present a protocol to label HIV-1 envelope (Env) glycoproteins on virions using genetic code expansion and click ...
Wang Xu, Narendra Kumar Gonepudi, Maolin Lu   +2 more
doaj   +1 more source

Protective effect of stromal Dickkopf-3 in prostate cancer: opposing roles for TGFBI and ECM-1 [PDF]

, 2018
Aberrant transforming growth factor–β (TGF-β) signaling is a hallmark of the stromal microenvironment in cancer. Dickkopf-3 (Dkk-3), shown to inhibit TGF-β signaling, is downregulated in prostate cancer and upregulated in the stroma in benign prostatic ...
A Dutta, A Prudova, B Croix St, C Zenzmaier, C Zenzmaier, CL Busceti, CP Goswami, CP Goswami, CY Fu, D Bello-DeOcampo, D Fong, D Romero, D Romero, D Romero, DA Barron, EJ Tokar, Emmanouil Stylianakis, F Abarzua, F Gao, G Ayala, G Untergasser, Giacomo Domenici, GN Thalmann, H Bauderlique-Le Roy, H Kumon, H Kumon, H Ross-Adams, Hoda Kardooni, Ignacio Zabalza, Irantzu Gorroño-Etxebarria, J Debnath, J Veeck, Jennifer H. Steel, JL Leonard, JN Shah, Jonathan Waxman, JP Webber, L Buscemi, LR Rohrschneider, M Augsten, M Bekhouche, M Hangai, M Piva, M Zhu, Maria dM Vivanco, Miriam Rabano, MM Webber, MP Ween, N Fujimoto, N Yu, P Smits, P Su, Robert M. Kypta, S Dennler, T Li, TD Schmittgen, VE Krupnik, Virginia Murillo-Garzón, VP Montecinos, WY Chen, X Wang, Y Gong, Y Kawano, Y Niu, Z Tu, Zainab Al Shareef   +65 more
core   +2 more sources