Results 11 to 20 of about 1,590,893 (316)
Cell-Based Assays for Smoothened Ubiquitination and Sumoylation. [PDF]
Methods Mol Biol, 2022 The Hedgehog (Hh) family of secreted proteins governs embryonic development and adult tissue homeostasis by regulating the abundance, localization, and activity of the GPCR family protein Smoothened (Smo). Smo trafficking and subcellular accumulation are controlled by multiple posttranslational modifications (PTMs) including phosphorylation ...
Han Y, Jiang J.
europepmc +3 more sources
Measuring SERCA-mediated calcium uptake in mouse muscle homogenates
STAR Protocols, 2023 Summary: This protocol employs the indo-1 Ca2+ fluorophore to quantify Ca2+ uptake by the sarco(endo)plasmic reticulum Ca2+ ATPase pump in murine muscle homogenates and allows for real-time kinetic measurement of Ca2+ mobilization within the muscle ...
Mia S. Geromella +2 more
doaj +1 more source
STAR Protocols, 2021 Summary: Ketohexokinase (KHK) catalyzes the first step of fructose metabolism. Inhibitors of KHK enzymatic activity are being evaluated in clinical trials for the treatment of non-alcoholic fatty liver disease (NAFLD) and diabetes.
Se-Hyung Park +8 more
doaj +1 more source
New cationic nanovesicular systems containing lysine-based surfactants for topical administration: Toxicity assessment using representative skin cell lines [PDF]
, 2013 Many strategies for treating diseases require the delivery of drugs into the cell cytoplasm following internalization within endosomal vesicles. Thus, compounds triggered by low pH to disrupt membranes and release endosomal contents into the cytosol are ...
Vinardell, M. Pilar +11 more
core +1 more source
Measurement of autophagy via LC3 western blotting following DNA-damage-induced senescence
STAR Protocols, 2022 Summary: Senescent cells accumulation is associated with aging and age-related diseases, and recent findings suggest that autophagy, the activity of the intracellular degradation system, decreases during senescence.
Hitomi Yamamoto-Imoto +3 more
doaj +1 more source
High-throughput mechanobiology: Force modulation of ensemble biochemical and cell-based assays [PDF]
, 2021 Mechanobiology is focused on how the physical forces and mechanical properties of proteins, cells, and tissues contribute to physiology and disease. Although the response of proteins and cells to mechanical stimuli is critical for function, the tools to ...
Pearson, DS +4 more
core +3 more sources
Protocol for single-molecule pull-down of fluorescently tagged oligomers from cell lysates
STAR Protocols, 2023 Summary: Mutations in intrinsically disordered proteins drive the irreversible formation of pathological aggregates, a hallmark of neurodegenerative diseases.
Nathalie Djaja, Sua Myong
doaj +1 more source
STAR Protocols, 2023 Summary: Here, we present a protocol for assessing metabolic activity of bacterial populations by measuring heat flow using isothermal calorimetry. We outline the steps for preparing the different growth models of Pseudomonas aeruginosa and performing ...
Katrin Beilharz +6 more
doaj +1 more source
, 2022 S.243-250Dynamic properties of cancer cells, most notably their ability to migrate, have been correlated successfully with their invasive nature in vivo. To establish a stronger experimental basis for such a correlation we subjected five different cancer
Kohidai, László +2 more
core +1 more source
Pseudotype-based neutralization assays for influenza: a systematic analysis [PDF]
, 2015 The use of vaccination against the influenza virus remains the most effective method of mitigating the significant morbidity and mortality caused by this virus.
Ferrara, Francesca +11 more
core +1 more source