Results 11 to 20 of about 1,374,920 (267)

Measuring SERCA-mediated calcium uptake in mouse muscle homogenates

STAR Protocols, 2023
Summary: This protocol employs the indo-1 Ca2+ fluorophore to quantify Ca2+ uptake by the sarco(endo)plasmic reticulum Ca2+ ATPase pump in murine muscle homogenates and allows for real-time kinetic measurement of Ca2+ mobilization within the muscle ...
Mia S. Geromella, Jessica L. Braun, Val A. Fajardo   +2 more
doaj   +1 more source

A luminescence-based protocol for assessing fructose metabolism via quantification of ketohexokinase enzymatic activity in mouse or human hepatocytes

STAR Protocols, 2021
Summary: Ketohexokinase (KHK) catalyzes the first step of fructose metabolism. Inhibitors of KHK enzymatic activity are being evaluated in clinical trials for the treatment of non-alcoholic fatty liver disease (NAFLD) and diabetes.
Se-Hyung Park, Robert N. Helsley, Leila Noetzli, Ho-Chou Tu, Kristina Wallenius, Gavin O’Mahony, Jeremie Boucher, Jianming Liu, Samir Softic   +8 more
doaj   +1 more source

Measurement of autophagy via LC3 western blotting following DNA-damage-induced senescence

STAR Protocols, 2022
Summary: Senescent cells accumulation is associated with aging and age-related diseases, and recent findings suggest that autophagy, the activity of the intracellular degradation system, decreases during senescence.
Hitomi Yamamoto-Imoto, Eiji Hara, Shuhei Nakamura, Tamotsu Yoshimori   +3 more
doaj   +1 more source

Protocol for single-molecule pull-down of fluorescently tagged oligomers from cell lysates

STAR Protocols, 2023
Summary: Mutations in intrinsically disordered proteins drive the irreversible formation of pathological aggregates, a hallmark of neurodegenerative diseases.
Nathalie Djaja, Sua Myong
doaj   +1 more source

Protocol to assess metabolic activity of Pseudomonas aeruginosa by measuring heat flow using isothermal calorimetry

STAR Protocols, 2023
Summary: Here, we present a protocol for assessing metabolic activity of bacterial populations by measuring heat flow using isothermal calorimetry. We outline the steps for preparing the different growth models of Pseudomonas aeruginosa and performing ...
Katrin Beilharz, Kasper Nørskov Kragh, Blaine Fritz, Julius B. Kirkegaard, Tim Tolker-Nielsen, Thomas Bjarnsholt, Mads Lichtenberg   +6 more
doaj   +1 more source

Protocol for imaging proteins associated with Legionella-containing vacuoles in host cells

STAR Protocols, 2021
Summary: The intracellular bacterial pathogen Legionella pneumophila exploits host cellular systems using approximately 300 effector proteins to establish a replicative niche known as the Legionella-containing vacuole (LCV).
Tomoe Kitao, Kohei Arasaki, Hiroki Nagai, Tomoko Kubori   +3 more
doaj   +1 more source

Titration and neutralizing antibody quantification by focus forming assay for Powassan virus

STAR Protocols, 2022
Summary: The development of high-throughput assays measuring Powassan virus (POWV) lineage I and II represents an important step in virological and immunological studies.
E. Taylor Stone, Alec J. Hirsch, Jessica L. Smith, James D. Brien, Amelia K. Pinto   +4 more
doaj   +1 more source

Fluorescence-based protocol for revealing cellular arrangement in biofilms

STAR Protocols, 2023
Summary: Standardized assays have greatly advanced the understanding of multicellular bacterial biofilms, but they lack cell-scale detail. Here, we present a fluorescence-based protocol that builds on past assays to reveal the cellular-scale arrangement ...
Devina Puri, Xin Fang, Kyle R. Allison
doaj   +1 more source

Relationship Between the Salivary Microbiome and Oral Malodor Metabolites in Older Thai Individuals with Periodontitis and the Cytotoxic Effects of Malodor Compounds on Human Oral Squamous Carcinoma (HSC-4) Cells

Dentistry Journal
Background/Objectives: Halitosis is primarily caused by the activity of oral microorganisms. In this study, we employed metagenomic sequencing and metabolomic approaches to investigate the differences in salivary microbiota and metabolite profiles ...
Witsanu Srila, Kritsana Sripilai, Thunwa Binlateh, Peungchaleoy Thammanichanon, Watcharaphol Tiskratok, Parinya Noisa, Paiboon Jitprasertwong   +6 more
doaj   +1 more source

A tissue culture infectious dose-derived protocol for testing of SARS-CoV-2 neutralization of serum antibodies on adherent cells

STAR Protocols, 2021
Summary: For a cytopathic virus such as severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2), the neutralization capacity of serum from convalescent or vaccinated persons or of therapeutic antibodies can be tested on adherent cell cultures ...
Fabio Hasler, Agathe Duda, Thomas M. Kündig, Pål Johansen   +3 more
doaj   +1 more source