STAR Protocols, 2023 Summary: N-Glycosylation is a common protein modification catalyzed by a series of glycosylation enzymes in the endoplasmic reticulum and Golgi apparatus.
Jie Li, Jianchao Zhang, Yanzhuang Wang
doaj +1 more source
Detection of DNA damage by alkaline comet assay in mouse colonic mucosa
STAR Protocols, 2021 Summary: We recently characterized the association between DNA damage and immunoresponse in vivo in colonic mucosa of mice infected with a Salmonella Typhimurium strain expressing a genotoxin, known as typhoid toxin.
Elisa Boutet-Robinet +4 more
doaj +1 more source
Protocol to measure inter-centrosome distance in adherent cells using epifluorescence microscopy
STAR Protocols, 2022 Summary: We present here a protocol to assay the centrosome separation events at late-G2 phase of the cell cycle by immunofluorescence microscopy. We describe the steps required for imaging and measurement of inter-centrosome distance.
Franco K.C. Au +2 more
doaj +1 more source
Measuring SERCA-mediated calcium uptake in mouse muscle homogenates
STAR Protocols, 2023 Summary: This protocol employs the indo-1 Ca2+ fluorophore to quantify Ca2+ uptake by the sarco(endo)plasmic reticulum Ca2+ ATPase pump in murine muscle homogenates and allows for real-time kinetic measurement of Ca2+ mobilization within the muscle ...
Mia S. Geromella +2 more
doaj +1 more source
STAR Protocols, 2021 Summary: Ketohexokinase (KHK) catalyzes the first step of fructose metabolism. Inhibitors of KHK enzymatic activity are being evaluated in clinical trials for the treatment of non-alcoholic fatty liver disease (NAFLD) and diabetes.
Se-Hyung Park +8 more
doaj +1 more source
A Monolithically Fabricated Combinatorial Mixer for Microchip-Based High-Throughput Cell Culturing Assays [PDF]
, 2007 We present an integrated method to fabricate 3- D microfluidic networks and fabricated the first on-chip cell culture device with an integrated combinatorial mixer.
Ho, Dean, Liu, Mike C., Tai, Yu-Chong
core +1 more source
Mitotic cell death induction by targeting the mitotic spindle with tubulin-inhibitory indole derivative molecules [PDF]
, 2017 Tubulin-targeting molecules are widely used cancer therapeutic agents. They inhibit microtubule-based structures, including the mitotic spindle, ultimately preventing cell division.
Coluccia, Antonio +10 more
core +1 more source
Measurement of autophagy via LC3 western blotting following DNA-damage-induced senescence
STAR Protocols, 2022 Summary: Senescent cells accumulation is associated with aging and age-related diseases, and recent findings suggest that autophagy, the activity of the intracellular degradation system, decreases during senescence.
Hitomi Yamamoto-Imoto +3 more
doaj +1 more source
Overview of Serological Techniques for Influenza Vaccine Evaluation: Past, Present and Future [PDF]
, 2014 Serological techniques commonly used to quantify influenza-specific antibodies include the Haemagglutination Inhibition (HI), Single Radial Haemolysis (SRH) and Virus Neutralization (VN) assays.
Callow +30 more
core +3 more sources
Protocol for single-molecule pull-down of fluorescently tagged oligomers from cell lysates
STAR Protocols, 2023 Summary: Mutations in intrinsically disordered proteins drive the irreversible formation of pathological aggregates, a hallmark of neurodegenerative diseases.
Nathalie Djaja, Sua Myong
doaj +1 more source