STAR Protocols, 2023 Summary: Here, we present a protocol for assessing metabolic activity of bacterial populations by measuring heat flow using isothermal calorimetry. We outline the steps for preparing the different growth models of Pseudomonas aeruginosa and performing ...
Katrin Beilharz +6 more
doaj +1 more source
A Morphological identification cell cytotoxicity assay using cytoplasm-localized fluorescent probe (CLFP) to distinguish living and dead cells [PDF]
, 2016 Cell cytotoxicity assays include cell activity assays and morphological identification assays. Currently, all frequently used cytotoxicity assays belong to cell activity assays but suffer from detection limitations.
Chen, Jialing +8 more
core +2 more sources
Sensing Cell-Culture Assays with Low-Cost Circuitry [PDF]
, 2018 An alternative approach for cell-culture end-point protocols is proposed herein. This new technique is suitable for real-time remote sensing. It is based on Electrical Cell-substrate Impedance Spectroscopy (ECIS) and employs the Oscillation-Based Test ...
Daza Navarro, María Paula +7 more
core +1 more source
Diamagnetic repulsion of particles for multilaminar flow assays [PDF]
, 2015 © The Royal Society of Chemistry. We demonstrate diamagnetic repulsion forces for performing continuous multilaminar flow assays on particles based on their intrinsic properties and with a simple setup. The platform could be applied to sandwich assays on
Elders, Luke T. +3 more
core +1 more source
Structural optimization and biological evaluation of 2-substituted 5-hydroxyindole-3-carboxylates as potent inhibitors of human 5-lipoxygenase. [PDF]
, 2009 Pharmacological suppression of leukotriene biosynthesis by inhibitors of 5-lipoxygenase (5-LO) is a strategy to intervene with inflammatory and allergic disorders.
BÜHRING U +8 more
core +1 more source
Activity-based detection of consumption of synthetic cannabinoids in authentic urine samples using a stable cannabinoid reporter system [PDF]
, 2017 Synthetic cannabinoids (SCs) continue to be the largest group of new psychoactive substances (NPS) monitored by the European Monitoring Center of Drugs and Drugs of Abuse (EMCDDA).
Auwärter, Volker +3 more
core +1 more source
Microfluidic System for Automated Cell-Based Assays [PDF]
JALA: Journal of the Association for Laboratory Automation, 2007 Microfluidic cell culture is a promising technology for applications in the drug screening industry. Key benefits include improved biological function, higher-quality cell-based data, reduced reagent consumption, and lower cost. In this work, we demonstrate how a microfluidic cell culture design was adapted to be compatible with the standard 96-well ...
Philip J, Lee +3 more
openaire +2 more sources
Protocol for imaging proteins associated with Legionella-containing vacuoles in host cells
STAR Protocols, 2021 Summary: The intracellular bacterial pathogen Legionella pneumophila exploits host cellular systems using approximately 300 effector proteins to establish a replicative niche known as the Legionella-containing vacuole (LCV).
Tomoe Kitao +3 more
doaj +1 more source
Titration and neutralizing antibody quantification by focus forming assay for Powassan virus
STAR Protocols, 2022 Summary: The development of high-throughput assays measuring Powassan virus (POWV) lineage I and II represents an important step in virological and immunological studies.
E. Taylor Stone +4 more
doaj +1 more source
CD171- and GD2-specific CAR-T cells potently target retinoblastoma cells in preclinical in vitro testing [PDF]
, 2019 BACKGROUND: Chimeric antigen receptor (CAR)-based T cell therapy is in early clinical trials to target the neuroectodermal tumor, neuroblastoma. No preclinical or clinical efficacy data are available for retinoblastoma to date.
Andersch, Lena +15 more
core +2 more sources