Results 31 to 40 of about 1,374,920 (267)

Protocol for the use of Oredsson universal replacement medium for cell banking and routine culturing of monolayer and suspension cultures

STAR Protocols
Summary: The Oredsson universal replacement (OUR) medium is the formulation of a universal xeno-free medium designed for the cultivation of human normal and cancer cells in 2D and 3D cultures.
Stina Oredsson, Atena Malakpour-Permlid, Johan Zhu, Tilo Weber   +3 more
doaj   +1 more source

Time-lapse live-cell imaging of pyroptosis by confocal microscopy

STAR Protocols, 2023
Summary: Pyroptosis is a type of regulated necrosis executed by gasdermin. Osmotic cell swelling and membrane perforation are the key features of pyroptosis.
Shuai Jiang, Kunpeng Qin, Li Sun
doaj   +1 more source

Microfluidics for cell-based assays

Materials Today, 2005
Microfluidic systems are powerful tools in chemistry, physics, and biology. The behavior of confined fluids differs from macroscopic systems and allows precise control of the chemical composition of fluids, as well as the temporal and spatial patterns of microscopic fluid elements.
Pihl, Johan, Sinclair, Jon, Karlsson, Mattias, Orwar, Owe   +3 more
openaire   +1 more source

The Role of “Adult‐Onset” Cancer Predisposition Genes in Pediatric Cancer: A Comprehensive Review

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Current literature estimates that 10% of pediatric cancers are caused by pathogenic or likely pathogenic (P/LP) germline variants in cancer predisposition genes (CPGs). Variants in CPGs thought to increase cancer risk exclusively during adulthood are referred to as “adult‐onset” CPGs (aoCPGs).
Maria Rozo, Miriam A. Bornhorst, Angela J. Waanders, Sara Zarnegar‐Lumley, Ashwin Lakshman Koppayi, Lucy A. Godley   +5 more
wiley   +1 more source

Protocol for assessing T cell receptor-mediated human T cell cytotoxicity

STAR Protocols
Summary: Measuring the cytotoxicity of human T cells is a valuable but challenging task. We present a protocol for assessing the ability of polyclonal human T cells to kill target cells upon T cell receptor (TCR) triggering.
Kathryne E. Marks, Ifeoluwakiisi Adejoorin, John Sowerby, Deepak A. Rao   +3 more
doaj   +1 more source

Protocol for dissection and culture of murine dorsal root ganglia neurons to study neuropeptide release

STAR Protocols, 2021
Summary: In this protocol, we provide step-by-step instructions for dissection and culture of primary murine dorsal root ganglia (DRG), which provide an opportunity to study the functional properties of peripheral sensory neurons in vitro.
Caroline Perner, Caroline L. Sokol
doaj   +1 more source

Early Impact of Childhood Opportunity on Neurocognitive Outcomes in Sickle Cell Disease

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Introduction Neurocognitive impairment is a well‐recognized complication of sickle cell disease (SCD) that begins early in childhood and persists across development. While cerebrovascular injury contributes substantially to risk, neurocognitive deficits are also observed in children without overt or silent cerebral infarctions, suggesting ...
Julia E. LaMotte, Hannah Koch, Olivia A. Coughlin, Jillian R. Bouck, Allie Carter, Seethal A. Jacob   +5 more
wiley   +1 more source

Protocol for precision cutting and short-term culture of lymph node tissue slices for modeling of innate immune responses ex vivo

STAR Protocols
Summary: Secondary lymphoid organs are highly organized for the initiation of immune responses. Here, we present a protocol that enables this microanatomical structure to be preserved during short-term culture, allowing ex vivo modeling of human innate ...
Joannah R. Fergusson, Mark Coles, Calliope A. Dendrou, Anita Milicic   +3 more
doaj   +1 more source

Visualizing Cellular Dynamics and Protein Localization in 3D Collagen

STAR Protocols, 2020
Summary: Immune cells migrate and communicate through cell-to-cell interactions and cytokines to coordinate the specificity and timing of the immune response.
Wan Hon Koh, Romaniya Zayats, Paul Lopez, Thomas T. Murooka   +3 more
doaj   +1 more source

A protocol for imaging microvilli biogenesis on the surface of cultured porcine kidney epithelial cell monolayers

STAR Protocols, 2021
Summary: A key facet of epithelial differentiation is the assembly of actin-based protrusions known as microvilli, which amplify apical membrane surface area for various cell functions. To probe mechanisms of microvillus assembly, we developed a protocol
Isabella M. Gaeta, Leslie M. Meenderink, Matthew J. Tyska   +2 more
doaj   +1 more source