Results 51 to 60 of about 27,881,538 (305)

Time-lapse live-cell imaging of pyroptosis by confocal microscopy

STAR Protocols, 2023
Summary: Pyroptosis is a type of regulated necrosis executed by gasdermin. Osmotic cell swelling and membrane perforation are the key features of pyroptosis.
Shuai Jiang, Kunpeng Qin, Li Sun
doaj   +1 more source

Microfluidics for cell-based assays

Materials Today, 2005
Microfluidic systems are powerful tools in chemistry, physics, and biology. The behavior of confined fluids differs from macroscopic systems and allows precise control of the chemical composition of fluids, as well as the temporal and spatial patterns of microscopic fluid elements.
Pihl, Johan, Sinclair, Jon, Karlsson, Mattias, Orwar, Owe   +3 more
openaire   +1 more source

Comparative Drug Response Profiling in Neuroblastoma Cell Lines and Patient‐Derived Tumor Organoids

Pediatric Blood &Cancer, EarlyView.
ABSTRACT High‐risk neuroblastoma remains a leading cause of pediatric cancer mortality, and improved preclinical models are needed to guide therapeutic developments. We screened seven high‐risk neuroblastoma cell lines and three patient‐derived tumor organoids with 528 compounds alongside bone marrow controls, and compared them with external datasets ...
Krzysztof Wierbiłowicz, Se Whee Sammy Park, Aironas Los, Ernest Liu, Nona Struyf, Tom Erkers, Sören Lehmann, Jan J. Molenaar, Jakob Stenman, Olli Kallioniemi, Päivi Östling, Brinton Seashore‐Ludlow, Kasper Karlsson   +12 more
wiley   +1 more source

Protocol for assessing T cell receptor-mediated human T cell cytotoxicity

STAR Protocols
Summary: Measuring the cytotoxicity of human T cells is a valuable but challenging task. We present a protocol for assessing the ability of polyclonal human T cells to kill target cells upon T cell receptor (TCR) triggering.
Kathryne E. Marks, Ifeoluwakiisi Adejoorin, John Sowerby, Deepak A. Rao   +3 more
doaj   +1 more source

Protocol for dissection and culture of murine dorsal root ganglia neurons to study neuropeptide release

STAR Protocols, 2021
Summary: In this protocol, we provide step-by-step instructions for dissection and culture of primary murine dorsal root ganglia (DRG), which provide an opportunity to study the functional properties of peripheral sensory neurons in vitro.
Caroline Perner, Caroline L. Sokol
doaj   +1 more source

Impact of Radiation Therapy on Physical and Psychosocial Health of Adolescents and Young Adults: A Joint Report From the Children's Oncology Group AYA and Radiation Oncology Committees

Pediatric Blood &Cancer, EarlyView.
ABSTRACT Rates of cancer among adolescents and young adults (AYA), age 15–39 years, are increasing. Consequently, radiation oncologists are treating more AYAs who have diagnoses spanning both pediatric and adult practices. Compared to pediatric and older adult patients, AYAs face a unique set of challenges.
Hesham Elhalawani, Rachel K. Peterson, Avani D. Rao, Laurie J. McKenzie, Ahmed Magdy Elmotayam, Leslie Chang, Michael Roth, Susan L. McGovern   +7 more
wiley   +1 more source

Identification of major factors influencing ELISpot-based monitoring of cellular responses to antigens from mycobacterium tuberculosis [PDF]

, 2009
A number of different interferon-c ELISpot protocols are in use in laboratories studying antigen-specific immune responses. It is therefore unclear how results from different assays compare, and what factors most significantly influence assay outcome ...
Pathan, Ansar A, Mascart Françoise, Hazel M. Dockrell, Simone A. Joosten, Steven G. Smith, Ottenhoff, THM, Françoise Mascart, Steven G Smith, Pathan Ansar A., Simone A Joosten, Verscheure, Virginie, Ottenhoff Tom H. M., Smith, SG, Hazel M Dockrell, Verscheure, V, Smith, Steven G, McShane Helen, Ottenhoff, TH, Virginie Verscheure, Pathan, AA, Tom H. M. Ottenhoff, Joosten, Simone A, Ansar A. Pathan, Ottenhoff, Tom HM, Tom H M Ottenhoff, Helen McShane, Ansar A Pathan, Joosten, SA, Mascart, F, Joosten Simone A., Ottenhoff, Tom H M, Mascart, Françoise, Dockrell Hazel M., Verscheure Virginie, Dockrell, HM, Dockrell, Hazel M, McShane, H, McShane, Helen, Smith Steven G.   +38 more
core   +2 more sources

Characterization of the anti-NMDAR1 autoantibodies using cell-based assays.

, 2021
(A) Human NMDAR1 proteins were expressed on HEK293 cells on BIOCHIPs purchased from Euroimmun. Both anti-Human NMDAR1 autoantibody (Euroimmun) and mouse anti-NMDAR1 antibody (BD, diluted at 1:10,000) recognize the NMDAR1 proteins on HEK293 cells in cell ...
Victoria Risbrough (8295138), Susan Powell (8542947), William Yue (11378218), Sorana Caldwell (423258), Xianjin Zhou (182433)   +4 more
core   +1 more source

Protocol for precision cutting and short-term culture of lymph node tissue slices for modeling of innate immune responses ex vivo

STAR Protocols
Summary: Secondary lymphoid organs are highly organized for the initiation of immune responses. Here, we present a protocol that enables this microanatomical structure to be preserved during short-term culture, allowing ex vivo modeling of human innate ...
Joannah R. Fergusson, Mark Coles, Calliope A. Dendrou, Anita Milicic   +3 more
doaj   +1 more source

Visualizing Cellular Dynamics and Protein Localization in 3D Collagen

STAR Protocols, 2020
Summary: Immune cells migrate and communicate through cell-to-cell interactions and cytokines to coordinate the specificity and timing of the immune response.
Wan Hon Koh, Romaniya Zayats, Paul Lopez, Thomas T. Murooka   +3 more
doaj   +1 more source