Results 61 to 70 of about 1,590,893 (316)

Visualizing Cellular Dynamics and Protein Localization in 3D Collagen

STAR Protocols, 2020
Summary: Immune cells migrate and communicate through cell-to-cell interactions and cytokines to coordinate the specificity and timing of the immune response.
Wan Hon Koh, Romaniya Zayats, Paul Lopez, Thomas T. Murooka   +3 more
doaj   +1 more source

A protocol for imaging microvilli biogenesis on the surface of cultured porcine kidney epithelial cell monolayers

STAR Protocols, 2021
Summary: A key facet of epithelial differentiation is the assembly of actin-based protrusions known as microvilli, which amplify apical membrane surface area for various cell functions. To probe mechanisms of microvillus assembly, we developed a protocol
Isabella M. Gaeta, Leslie M. Meenderink, Matthew J. Tyska   +2 more
doaj   +1 more source

Efficacy and Safety Analysis of Roxarestat in Regulating Renal Anemia in Patients on Maintenance Hemodialysis

Therapeutic Apheresis and Dialysis, EarlyView.
ABSTRACT Objective To compare the efficacy and safety of roxarestat versus recombinant human erythropoietin (rhEPO) in the management of renal anemia in patients undergoing maintenance hemodialysis. Methods This was a prospective, open‐label, randomized controlled trial.
Lingling Chen, Junjie Zhu, Qiaonan Ge
wiley   +1 more source

Fluorescence/luminescence-based markers for the assessment of Schistosoma mansoni schistosomula drug assays [PDF]

, 2015
Schistosomiasis is responsible for a tremendous public health burden, yet only a single drug, praziquantel, is available. New antischistosomal treatments should therefore be developed. The accuracy, speed and objectivity of in vitro drug screening depend
Gordana Panic, Jennifer Keiser, Flores, Dayana, Keiser, Jennifer, Ingram-Sieber, Katrin, Dayana Flores, Katrin Ingram-Sieber, Panic, Gordana   +7 more
core   +1 more source

Therapeutic Apheresis for Intravenous Methylprednisolone‐Refractory Neuromyelitis Optica Spectrum Disorder: Clinical and Radiological Outcomes in a Single‐Center Case Series

Therapeutic Apheresis and Dialysis, EarlyView.
ABSTRACT Background Neuromyelitis optica spectrum disorder (NMOSD) is a relapsing autoimmune disease of the central nervous system. High‐dose intravenous methylprednisolone (IVMP) is the standard first‐line therapy for acute attacks, although some patients remain refractory.
Wataru Horiguchi, Daisuke Katagiri, Takeyuki Watadani, Shunichi Matsuda, Hiroshi Kaneko, Noritoshi Arai   +5 more
wiley   +1 more source

Investigating the reproducibility of in vitro cell biology assays using mathematical models

, 2017
In vitro cell biology assays are routinely used to study cancer spreading, drug design and tissue repair. However, issues associated with reproducibility are reported in literature.
Jin, Wang
core   +1 more source

Protocol for optimizing production and quality control of infective EcoHIV virions

STAR Protocols, 2023
Summary: EcoHIV is a model of HIV infection that recapitulates aspects of HIV-1 pathology in mice. However, there are limited published protocols to guide EcoHIV virion production.
Hammodah R. Alfar, Daphne N. Pariser, Harry Chanzu, Smita Joshi, Daniëlle M. Coenen, Joshua Lykins, Kanakanagavalli Shravani Prakhya, Mary Jane Potash, Wei Chao, Jennifer Kelschenbach, David J. Volsky, Kelly Metcalf-Pate, Sidney W. Whiteheart   +12 more
doaj   +1 more source

Effects of the Fluid Replacement Method During Online Hemodiafiltration on the Solute Removal Performance and Biocompatibility Using the Asymmetric Cellulose Triacetate Membrane

Therapeutic Apheresis and Dialysis, EarlyView.
ABSTRACT Introduction Pre‐dilution online hemodiafiltration (Pre‐HDF) is predominantly used in Japan, whereas post‐dilution online HDF (Post‐HDF) is more common in Europe. An asymmetric cellulose triacetate (ATA) membrane may improve biocompatibility.
Kenji Sakurai, Yoshitaka Kurihara, Fumi Yamauchi, Hiromi Hosoya, Takeshi Saito   +4 more
wiley   +1 more source

Natural mineral particles are cytotoxic to rainbow trout gill epithelial cells in vitro [PDF]

, 2014
Worldwide increases in fluvial fine sediment are a threat to aquatic animal health. Fluvial fine sediment is always a mixture of particles whose mineralogical composition differs depending on the sediment source and catchment area geology.Nonetheless ...
Pietsch, Constanze, Michel, C., Herzog, S., De Capitani, C., Christian de Capitani, Pietsch, C., Simon Herzog, Burkhardt-Holm, Patricia, Patricia Burkhardt-Holm (592071), Constanze Pietsch, Christian Michel (159009), Patricia Burkhardt-Holm, de Capitani, Christian, Herzog, Simon, Burkhardt-Holm, P., Simon Herzog (592069), Christian de Capitani (592070), Michel, Christian, Constanze Pietsch (592072), Christian Michel   +19 more
core   +1 more source

T-B coculture assay for functional analysis of antigen-specific memory CD4+ T cells

STAR Protocols
Summary: The B cell “help” function of CD4+ T cells is critical in establishing the humoral arm of adaptive immunity. Here, we present a protocol to measure the “help” function of antigen-specific memory T cells using an autologous T-B coculture ...
Asgar Ansari, Poonam Coshic, Ashok Sharma, Alessandro Sette, Daniela Weiskopf, Nimesh Gupta   +5 more
doaj   +1 more source