Results 61 to 70 of about 1,974,930 (309)

Protocol for in vitro assessment of human monocyte transendothelial migration using a high-throughput live cell imaging system

open access: yesSTAR Protocols, 2023
Summary: In vitro modeling of the different steps of immune cell recruitment is essential to decipher the role of endothelial cells in this process. Here, we present a protocol for the assessment of human monocyte transendothelial migration using a live ...
Ségolène Ladaigue   +6 more
doaj   +1 more source

Selective glucocorticoid receptor properties of GSK866 analogs with cysteine reactive warheads [PDF]

open access: yes, 2017
Synthetic glucocorticoids (GC) are the mainstay therapy for treatment of acute and chronic inflammatory disorders. Due to the high adverse effects associated with long-term use, GC pharmacology has focused since the nineties on more selective GC ligand ...
Atucha   +61 more
core   +2 more sources

Protocol for the use of Oredsson universal replacement medium for cell banking and routine culturing of monolayer and suspension cultures

open access: yesSTAR Protocols
Summary: The Oredsson universal replacement (OUR) medium is the formulation of a universal xeno-free medium designed for the cultivation of human normal and cancer cells in 2D and 3D cultures.
Stina Oredsson   +3 more
doaj   +1 more source

Time-lapse live-cell imaging of pyroptosis by confocal microscopy

open access: yesSTAR Protocols, 2023
Summary: Pyroptosis is a type of regulated necrosis executed by gasdermin. Osmotic cell swelling and membrane perforation are the key features of pyroptosis.
Shuai Jiang, Kunpeng Qin, Li Sun
doaj   +1 more source

Pan-Cancer Analysis of lncRNA Regulation Supports Their Targeting of Cancer Genes in Each Tumor Context [PDF]

open access: yes, 2018
Long noncoding RNAs (lncRNAs) are commonly dys-regulated in tumors, but only a handful are known toplay pathophysiological roles in cancer. We inferredlncRNAs that dysregulate cancer pathways, onco-genes, and tumor suppressors (cancer genes) bymodeling ...
Abdel-Rahman, Mohamed H.   +736 more
core   +3 more sources

Sirolimus for Extracranial Arteriovenous Malformations: A Scoping Review of the Evidence in Syndromic and Non‐Syndromic Cases

open access: yesPediatric Blood &Cancer, EarlyView.
ABSTRACT Arteriovenous malformations (AVMs) are rare, high‐flow, vascular anomalies that can occur either sporadically or as part of a genetic syndrome. AVMs can progress with serious morbidity and even mortality if left unchecked. Sirolimus is an mTOR inhibitor that is effective in low‐flow vascular malformations; however, its role in AVMs is unclear.
Will Swansson   +3 more
wiley   +1 more source

Protocol for assessing T cell receptor-mediated human T cell cytotoxicity

open access: yesSTAR Protocols
Summary: Measuring the cytotoxicity of human T cells is a valuable but challenging task. We present a protocol for assessing the ability of polyclonal human T cells to kill target cells upon T cell receptor (TCR) triggering.
Kathryne E. Marks   +3 more
doaj   +1 more source

Protocol for optimizing production and quality control of infective EcoHIV virions

open access: yesSTAR Protocols, 2023
Summary: EcoHIV is a model of HIV infection that recapitulates aspects of HIV-1 pathology in mice. However, there are limited published protocols to guide EcoHIV virion production.
Hammodah R. Alfar   +12 more
doaj   +1 more source

Ceramic materials lead to underestimated DNA quantifications : a method for reliable measurements [PDF]

open access: yes, 2010
In the context of investigating cell-material interactions or of material-guided generation of tissues, DNA quantification represents an elective method to precisely assess the number of cells attached or embedded within different substrates. Nonetheless,
Martin, I., Piccinini, E., Sadr, N.
core   +1 more source

Preferences of Pediatric Patients and Their Caregivers for Chemotherapy‐Induced Nausea and Vomiting Control Endpoints: A Mixed Methods Study

open access: yesPediatric Blood &Cancer, EarlyView.
ABSTRACT Purpose Although not always achieved, complete chemotherapy‐induced nausea and vomiting (CINV) control is the conventional goal of CINV prophylaxis. In this two‐center, mixed‐methods study, we sought to understand the preferences of adolescent patients and family caregivers for CINV control endpoints.
Haley Newman   +8 more
wiley   +1 more source

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