Results 21 to 30 of about 231,120 (294)

RNA-aptamers-in-droplets (RAPID) high-throughput screening for secretory phenotypes. [PDF]

, 2017
Synthetic biology and metabolic engineering seek to re-engineer microbes into living foundries for the production of high value chemicals. Through a design-build-test cycle paradigm, massive libraries of genetically engineered microbes can be constructed
Abate, Adam, Abatemarco, Joseph, Alper, Hal, Hassouneh, Wafa, Lin, Jyun-Liang, Liu, Leqian, Sarhan, Maen, Wagner, James, Yuan, Shuo-Fu   +8 more
core   +1 more source

Protocol for cell-based screening assay to measure ERK1/2 phosphorylation as a readout for complement receptor activation

STAR Protocols, 2023
Summary: The complement receptors C3aR and C5aR1 are promising therapeutic targets. Here, we present a protocol to screen the effects of different agonists and antagonists on these receptors in vitro, using phosphorylated extracellular signal-regulated ...
Xaria X. Li, Trent M. Woodruff
doaj   +1 more source

In-silico Predictive Mutagenicity Model Generation Using Supervised Learning Approaches [PDF]

, 2011
With the advent of High Throughput Screening techniques, it is feasible to filter possible leads from a mammoth chemical space that can act against a particular target and inhibit its action.
Abhik Seal, Abhik Seal, Anurag Passi, OSDD Consortium, UC Abdul Jaleel   +4 more
core   +2 more sources

Clinical application of high throughput molecular screening techniques for pharmacogenomics. [PDF]

, 2011
Genetic analysis is one of the fastest-growing areas of clinical diagnostics. Fortunately, as our knowledge of clinically relevant genetic variants rapidly expands, so does our ability to detect these variants in patient samples.
Schrijver, Iris, Wiita, Arun P
core   +1 more source

Assessment of protein inclusions in cultured cells using automated image analysis

STAR Protocols, 2022
Summary: Proteinaceous inclusions are associated with neurodegenerative diseases and cell models are often used to determine genetic and chemical modifiers of their formation.
Luke McAlary, Victoria K. Shephard, Mine Sher, Lauren J. Rice, Justin J. Yerbury, Neil R. Cashman, Steven S. Plotkin   +6 more
doaj   +1 more source

Semiconductor Electronic Label-Free Assay for Predictive Toxicology. [PDF]

, 2016
While animal experimentations have spearheaded numerous breakthroughs in biomedicine, they also have spawned many logistical concerns in providing toxicity screening for copious new materials. Their prioritization is premised on performing cellular-level
Chui, Chi On, Ji, Zhaoxia, Mao, Yufei, Meng, Huan, Shin, Kyeong-Sik, Wang, Xiang   +5 more
core   +2 more sources

Multiplex Profiling of Cellular Invasion in 3D Cell Culture Models. [PDF]

, 2012
To-date, most invasion or migration assays use a modified Boyden chamber-like design to assess migration as single-cell or scratch assays on coated or uncoated planar plastic surfaces.
Burgstaller, Gerald, Eickelberg, Oliver, Koch, Ina, Lindner, Michael, Oehrle, Bettina   +4 more
core   +8 more sources

Alginate-based 3D cancer cell culture for therapeutic response modeling

STAR Protocols, 2021
Summary: Two-dimensional (2D) culture of tumor cells fails to recapitulate some important aspects of cellular organization seen in in vivo experiments. In addition, cell cultures traditionally use non-physiological concentration of nutrients.
Farideh Davoudi, Samar Ghorbanpoor, Satoshi Yoda, Xiao Pan, Giovanna Stein Crowther, Xunqin Yin, Ellen Murchie, Aaron N. Hata, Henning Willers, Cyril H. Benes   +9 more
doaj   +1 more source

Canvass: a crowd-sourced, natural-product screening library for exploring biological space [PDF]

, 2018
NCATS thanks Dingyin Tao for assistance with compound characterization. This research was supported by the Intramural Research Program of the National Center for Advancing Translational Sciences, National Institutes of Health (NIH). R.B.A.
Andrade, Rodrigo B., Bougie, Danielle, Boxer, Matthew B., Brimacombe, Kyle R., Brown, Lauren E., Brown, M Kevin, Burns, Noah Z., Cha, Jin K., Cheff, Dorian M., Cheng, Ken, Clardy, Jon, Clement, Jason A., Coussens, Nathan P., Crooks, Peter A., Cuny, Gregory D., Dillon, Christopher, Dorjsuren, Dorjbal, Eastman, Richard T., Ganor, Jake, Garg, Neil K., Goess, Brian C., Grossman, Robert B., Guha, Rajarshi, Hall, Matthew D., Henderson, Mark J., Huang, Ruili, Hughes, Chambers C., Iannotti, Michael J., Inglese, James, Itkin, Zina, Jadhav, Ajit, Johnston, Jeffrey N., Joullie, Madeleine M., Karavadhi, Surendra, Kearney, Sara E., Kinghorn, A. Douglas, Kingston, David G. I., Klumpp-Thomas, Carleen, Krische, Michael J., Kwon, Ohyun, Lee, Tobie D., Lynch, Caitlin, Maimone, Thomas J., Majumdar, Susruta, Maloney, Katherine N., Mevers, Emily E., Michael, Sam, Mohamed, Enas, Moreno, Jesus, Morrill, Lucas A., Murphy, Brian T., Nagorny, Pavel, Olson, David E., Overman, Larry E., Picazo, Elias, Porco, John A., Ren, Tianjing, Rivas, Fatima, Rohde, Jason M., Ross, Samir A., Roth, Jacob S., Sakamuru, Srilatha, Sarpong, Richmond, Sharma, Indrajeet, Shaw, Jared T., Shen, Ben, Shen, Min, Shi, Wei, Shinn, Paul, Simeonov, Anton, Snyder, John K., Stephenson, Corey R. J., Sun, Wei, Susick, Robert B., Tan, Derek S., Tang, Yi, Taylor, Richard E., Thomson, Regan J., Titus, Steven A., Verano, Alyssa L., Vosburg, David A., Wan, Kanny K., Wu, Jimmy, Wuest, William M., Xia, Menghang, Xu, Zhengren, Yasgar, Adam, Zahoranszky-Kohalmi, Gergely, Zakarian, Armen, Zhang, Ya-Qin, Zhang, Yufeng, Zhao, Jinghua, Zhao, Tongan, Zheng, Wei, Zuo, Zhong   +94 more
core   +1 more source

Advancing liquid atmospheric pressure matrix-assisted laser desorption/ionization mass spectrometry toward ultra-high-throughput analysis [PDF]

, 2020
Label-free high-throughput screening using mass spectrometry has the potential to provide rapid large-scale sample analysis at a speed of more than one sample per second.
Brown, Jeffery, Cramer, Rainer, Hartmane, Evita, Krenkel, Henriette, Morris, Michael, Piras, Cristian   +5 more
core   +1 more source