Results 41 to 50 of about 137,634 (294)
Frontiers in Microbiology, 2016 Nicotinamidases catalyze the hydrolysis of the amide bond in nicotinamide to produce ammonia and nicotinic acid. These enzymes are an essential component of the NAD+ salvage pathway and are implicated in the viability of several pathogenic organisms. Its
Rubén Zapata-Pérez +4 more
doaj +1 more source
Quantification of drug-induced fractional killing using high-throughput microscopy
STAR Protocols, 2021 Summary: Anti-cancer drugs kill only a fraction of cells within a population at any given time. Here, we describe a protocol to quantify drug-induced fractional killing over time using high-throughput imaging.
Zintis Inde +2 more
doaj +1 more source
STAR Protocols, 2022 Summary: Well-characterized small molecules enable the study of cell processes and facilitate target validation. Here, we describe a high-content multiplex screen to investigate cell viability over 48 h, which can be combined with investigating ...
Amelie Tjaden +4 more
doaj +1 more source
Arbitrarily Accessible 3D Microfluidic Device for Combinatorial High-Throughput Drug Screening
Sensors, 2016 Microfluidics-based drug-screening systems have enabled efficient and high-throughput drug screening, but their routine uses in ordinary labs are limited due to the complexity involved in device fabrication and system setup.
Zhuofa Chen +6 more
doaj +1 more source
FEBS Letters, EarlyView.We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee +3 more
wiley +1 more source
Statistical Analysis of High Content Screening Data [PDF]
, 2009 High throughput screening experiments are typically used within the pharmaceutical industry for the identification and evaluation of candidate drugs. Using a high throughput screen with automated imaging platform allows a large number of compounds to be ...
Jacques, Richard
core
Protocol to identify small-molecule inhibitors against cancer drug resistance
STAR ProtocolsSummary: Drug resistance has emerged as a critical challenge in clinical cancer treatment. Here, we present a high-throughput screening protocol to identify therapeutic small-molecule inhibitors against drug-resistant cancer cells.
Juan Wei +6 more
doaj +1 more source
Development of a High Throughput Platform for Screening Glycoside Hydrolases based on Oxime-NIMS
Frontiers in Bioengineering and Biotechnology, 2015 Cost effective hydrolysis of biomass into sugars for biofuel production requires high-performance low-cost glycoside hydrolase (GH) cocktails that are active under demanding process conditions.
Kai eDeng +36 more
doaj +1 more source
FEBS Letters, EarlyView.Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes +4 more
wiley +1 more source
Protocol for screening inhibitors of the fungal sphingolipid-metabolizing enzyme ceramide synthase
STAR ProtocolsSummary: Fungal sphingolipids have emerged as potent antifungal targets based on cumulative evidence demonstrating that fungal sphingolipid metabolism is essential to the virulence of pathogenic fungi. Here, we present a protocol to screen for inhibitors
Andy Zhong +4 more
doaj +1 more source