Results 61 to 70 of about 231,120 (294)
STAR ProtocolsSummary: The interaction between cell surface receptors and their ligands is crucial for intercellular communication. However, current techniques for identifying direct receptor-ligand interactions remain limited.
Xiaoli Hu, Yiguo Wang
doaj +1 more source
Image-based dynamic phenotyping reveals genetic determinants of filamentation-mediated beta-lactam tolerance [PDF]
, 2020 Antibiotic tolerance characterized by slow killing of bacteria in response to a drug can lead to treatment failure and promote the emergence of resistance.
Camacho, Rafael +8 more
core +2 more sources
FEBS Letters, EarlyView.We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee +3 more
wiley +1 more source
High-throughput screening assay used in pharmacognosy: Selection, optimization and validation of methods of enzymatic inhibition by UV-visible spectrophotometry [PDF]
Journal of Pharmacy & Pharmacognosy Research, 2014 In research laboratories of both organic synthesis and extraction of natural products, every day a lot of products that can potentially introduce some biological activity are obtained.
Graciela Granados-Guzmán +3 more
doaj
Organoid drug screening in cancer: advances in high-throughput platforms and clinical applications [PDF]
OrganoidPatient-derived tumor organoids represent the most physiologically relevant 3-dimensional (3D) cancer models, preserving tumor heterogeneity and native tissue architecture.
Jiho Lee, Hyun Ju Kang, Jin-Ku Lee
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Journal of Chemistry, 2021 The effects of reaction conditions on the spectrophotometric and fluorometric assays using alternative substrates (p-nitrophenyl palmitate and 4-methylumbelliferyl oleate) were investigated to optimize them for the high-throughput screening of lipase ...
Jun-Young Park +4 more
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STAR Protocols, 2021 Summary: Histone deacetylases (HDACs) are ubiquitous enzymes that cleave post-translational ε-N-acyllysine modifications. The continued identification of diverse acyl modifications at lysine residues in proteins has resulted in discovery of new insight ...
Carlos Moreno-Yruela, Christian A. Olsen
doaj +1 more source
Subtyping of Swine Influenza Viruses Using a High-Throughput Real-Time PCR Platform [PDF]
, 2018 Influenza A viruses (IAVs) are important human and animal pathogens with high impact on human and animal health. In Denmark, a passive surveillance program for IAV in pigs has been performed since 2011, where screening tests and subsequent subtyping are ...
Breum, Solvej Ø. +6 more
core +3 more sources
FEBS Letters, EarlyView.Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes +4 more
wiley +1 more source
High-throughput mass spectrometry based assays for detection of reactive compounds
Green Analytical ChemistryHigh-throughput screening (HTS) is a foundational approach in small molecule drug discovery, but false positives due to reactive compounds can waste resources and hinder progress. Nuisance assays are therefore required to identify such compounds; however,
Fan Pu +7 more
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