Results 81 to 90 of about 137,634 (294)

Interpreting the effects of DNA polymerase variants at the structural level

Molecular Oncology, EarlyView.
Using MAVISp and molecular dynamics simulations, we analyzed over 60 000 missense variants in POLE and POLD1 from ClinVar, COSMIC, cBioPortal, and saturation mutagenesis. Identified mechanistic indicators, including stability, binding, and long‐range, enable structural interpretation, providing ACMG‐like evidence for possible reclassification of VUS ...
Matteo Arnaudi, Karolina Krzesińska, Ludovica Beltrame, Pablo Sánchez‐Izquierdo Besora, Matteo Tiberti, Mef Nilbert, Anna Rohlin, Elena Papaleo   +7 more
wiley   +1 more source

Complementary approaches to target human respiratory syncytial virus using small molecule ligands [PDF]

, 2012
In the current project, situated at the interface of chemistry and virology, complementary drug discovery approaches (high-throughput screening and structurebased drug design) have been exploited in order to discover novel small molecule inhibitors of ...
Lardeau, Charles-Hugues Claude Jean
core  

Differential Assay for High-Throughput Screening of Antibacterial Compounds

SLAS Discovery, 2007
The previously described Bacillus subtilis reporter strain BAU-102 is capable of detecting cell wall synthesis inhibitors that act at all stages of the cell wall synthesis pathway. In addition, this strain is capable of detecting compounds with hydrophobic/surfactant activity and alternative mechanisms of cell wall disruption.
Shaun P, Falk, Andrew T, Ulijasz, Bernard, Weisblum   +2 more
openaire   +2 more sources

Adapting High-Throughput Screening Methods and Assays for Biocontainment Laboratories [PDF]

ASSAY and Drug Development Technologies, 2015
High-throughput screening (HTS) has been integrated into the drug discovery process, and multiple assay formats have been widely used in many different disease areas but with limited focus on infectious agents. In recent years, there has been an increase in the number of HTS campaigns using infectious wild-type pathogens rather than surrogates or ...
Rasmussen, L, Tigabu, B, White, EL, Bostwick, R, Tower, N, Bukreyev, A, Rockx, B, LeDuc, JW, Noah, JW   +8 more
openaire   +3 more sources

Liquid biopsy‐based diagnostic evaluation of hypermethylated CpG sites for ovarian cancer diagnosis

Molecular Oncology, EarlyView.
This schematic outlines the workflow from biomarker identification to duplex MethyLight assay validation for epithelial ovarian cancer diagnosis using cfDNA‐based liquid biopsy. Initial screening of hypermethylated CpG candidates (cg02957270, cg10061138 cg00480298, COL2A1) was performed in tissue using ARMS‐PCR, COBRA, qPCR and image analysis. Selected
Deepa Bisht, Sameer Gupta, Amrita Chaurasia, Manisha Sachan   +3 more
wiley   +1 more source

Combination of small molecule microarray and confocal microscopy techniques for live cell staining fluorescent dye discovery

, 2013
Discovering new fluorochromes is significantly advanced by high-throughput screening (HTS) methods. In the present study a combination of small molecule microarray (SMM) prescreening and confocal laser scanning microscopy (CLSM) was developed in order to
Bhavyashree Suresh, Ferhan Ayaydin, Bokros, Attila, Eszter Molnár, Peksel, Begüm, Ágnes Medgyesi, Liliána Fehér, Pradeep Kumar Reddy Cingaram, Puskás, László G., Soujanya Kuntam, Attila Bokros, Begüm Peksel, Gabriella Fábián, Molnár, Eszter, László G. Puskás, Kuntam, Soujanya, Liliána Z. Fehér, Fábián, Gabriella, László Puskás, Cingaram, Pradeep Kumar Reddy, Pradeep Cingaram   +20 more
core   +1 more source

POLARISED views and FRETting about probe modulation assays: Learning from High Throughput Screening

SLAS Discovery
Fluorescent probe modulation assays are a widely used approach to monitor displacement or stabilisation of fluorescently labelled tool ligands by test compounds.
Alice Lanne, Catherine Bardelle, Gareth Davies, Antonia Turberville, Hannah Semple, Rachel Moore, Geoffrey A. Holdgate   +6 more
doaj   +1 more source

Epigenetic heterogeneity and plasticity in therapy‐induced tumor states through single‐cell multi‐omics

Molecular Oncology, EarlyView.
Single‐cell multi‐omics reveals epigenetic heterogeneity across therapy‐adaptive tumor states, including quiescent/dormant, drug‐tolerant persister, and EMT‐like phenotypes. By linking regulatory features with state‐associated biomarkers, these approaches inform biomarker‐guided therapeutic strategies for evolving tumors.
Hee Jung Kim, Hwiyeong Lee, Jin Hong, Daechan Park   +3 more
wiley   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

RNAi screening of Drosophila (Sophophora) melanogaster S2 cells for ricin sensitivity and resistance [PDF]

, 2011
The ribosome-inhibiting toxin ricin binds exposed β1→4 linked galactosyls on multiple glycolipids and glycoproteins on the cell surface of most eukaryotic cells. After endocytosis, internal cell trafficking is promiscuous, with only a small proportion of
Omar, Mahmoud M., Spooner, Robert A., Robert A. Spooner, Kevin G. Moffat, Sean T. Sweeney, De, Antara, Pawar, Vidya, Lord, Mike, Laura Briggs, Antara De, Lynne M. Roberts, Briggs, Laura, Roberts, L. M. (Lynne M.), Moffat, Kevin G., Vidya Pawar, Sweeney, Sean T., Mahmoud M. Omar, J. Michael Lord   +17 more
core   +1 more source