Results 101 to 110 of about 137,634 (294)
High-throughput production of human proteins for crystallization: the SGC experience.
, 2010 Producing purified human proteins with high yield and purity remains a considerable challenge. We describe the methods utilized in the Structural Genomics Consortium (SGC) in Oxford, resulting in successful purification of 48% of human proteins attempted;
Savitsky, P +24 more
core +1 more source
, 2023 Identifying high quality chemical starting points is a critical and challenging step in drug discovery, which typically involves screening large compound libraries or repurposing of compounds with known mechanisms of actions (MoAs).
Claude, Shelton IV +27 more
core +1 more source
FEBS Open Bio, EarlyView.The dual roles of CC and CXC chemokines in distinguishing active, latent, and subclinical tuberculosis were reviewed, along with an evaluation of their potential as diagnostic biomarkers and therapeutic targets to advance precision medicine in tuberculosis management. The graphical abstract was generated with AI assistance (Gemini 3.0).
Xuying Yin, Dangsheng Xiao, Jiezuan Yang
wiley +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
High-throughput approaches to investigate neutral lipid biosynthesis
, 2010 Neutral lipids are ubiquitously found in nature, serving primarily as energy storage compounds. The ability to control the rate of neutral lipid bioassembly is of particular interest in numerous applications.
Siloto, Rodrigo M. P. +1 more
core +1 more source
Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells
FEBS Open Bio, EarlyView.Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim +4 more
wiley +1 more source
High-throughput screening data for estrogen receptor model
, 2018 Estrogen receptor model data from the manuscript titled Integrated Model of Chemical Perturbations of Biological Pathways Using 18 In Vitro High-throughput Screening Assays for the Estrogen Receptor (Judson et al) published in Toxicological Science.
EPA's National Center for Computational Toxicology (5039467)
core +1 more source
High-Throughput Screening Assays to Identify Plant Natural Products with Antifungal Properties Against Fusarium oxysporum. [PDF]
Methods Mol Biol, 2022 Zhang Y +5 more
europepmc +1 more source
Aging Is a Key Driver for Adult Acute Myeloid Leukemia
Aging and Cancer, EarlyView.Acute myeloid leukemia (AML) is a classical age‐related hematologic malignancy, and a key driver of AML is aging, which profoundly regulates intrinsic factors such as genomic instability, epigenetic reprogramming, and metabolic dysregulation, and alters bone marrow microenvironment.
Rong Yin, Haojian Zhang
wiley +1 more source
A High Throughput Apoptosis Assay using 3D Cultured Cells
Molecules, 2019 A high throughput apoptosis assay using 3D cultured cells was developed with a micropillar/microwell chip platform. Live cell apoptosis assays based on fluorescence detection have been useful in high content screening.
Sang-Yun Lee, Il Doh, Dong Woo Lee
doaj +1 more source