Results 91 to 100 of about 231,120 (294)

Interaction of HS1BP3 with cortactin modulates TKS5 localisation, cell secretion and cancer malignancy

Molecular Oncology, EarlyView.
Here, we demonstrate that HS1BP3 interacts with Cortactin through a proline‐rich region (PRR3.1) and show that this interaction, and HS1BP3 itself, promote cancer cell proliferation and invasion. Inhibition of this interaction leads to build‐up of TKS5 in multivesicular endosomes and altered secretion of CD63 and CD9, providing an explanation for the ...
Arja Arnesen Løchen, Kristiane Søreng, Chiara Veroni, Laura Trachsel‐Moncho, Nagham Asp, Robin Gaupset, Lars Gustav Lyckander, Helene Knævelsrud, Lars Eftang, Anne Simonsen   +9 more
wiley   +1 more source

High-throughput screening in larval zebrafish identifies novel potent sedative-hypnotics [PDF]

, 2018
BACKGROUND: Many general anesthetics were discovered empirically, but primary screens to find new sedative-hypnotics in drug libraries have not used animals, limiting the types of drugs discovered.
Brown, Lauren E., Dai, Jennifer B., Daigle, Renee, Forman, Stuart A., Halpin, Elizabeth S., Jounaidi, Youssef, Marte-Oquendo, Francisco, Porco, John A., Schaus, Scott E., Trilles, Richard, Xu, Wenqing, Yang, Xiaoxuan, Yu, Buwei   +12 more
core   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

POLARISED views and FRETting about probe modulation assays: Learning from High Throughput Screening

SLAS Discovery
Fluorescent probe modulation assays are a widely used approach to monitor displacement or stabilisation of fluorescently labelled tool ligands by test compounds.
Alice Lanne, Catherine Bardelle, Gareth Davies, Antonia Turberville, Hannah Semple, Rachel Moore, Geoffrey A. Holdgate   +6 more
doaj   +1 more source

Pathways Across the Valley of Death: Novel Intellectual Property Strategies for Accelerated Drug Discovery [PDF]

, 2008
Drug discovery is stagnating. Government agencies, industry analysts, and industry scientists have all noted that, despite significant increases in pharmaceutical R&D funding, the production of fundamentally new drugs - particularly drugs that work on ...
Crossman, Colin, Rai, Arti K., Reichman, Jerome H., Uhlir, Paul F.   +3 more
core   +1 more source

High-Throughput Drug Screening Identifies a Potent Wnt Inhibitor that Promotes Airway Basal Stem Cell Homeostasis. [PDF]

, 2020
Mechanisms underpinning airway epithelial homeostatic maintenance and ways to prevent its dysregulation remain elusive. Herein, we identify that β-catenin phosphorylated at Y489 (p-β-cateninY489) emerges during human squamous lung cancer progression ...
Aros, Cody J, Bisht, Bharti, Chen, Michelle W, Damoiseaux, Robert, France, Bryan, Gomperts, Brigitte N, Meneses, Luisa K, Pantoja, Carla J, Paul, Manash K, Rickabaugh, Tammy M, Sandlin, Jenna M, Shia, David W, Tse, Jonathan A, Vijayaraj, Preethi   +13 more
core   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

High throughput screening using enzyme assay microarrays

Proceedings of the Second Joint 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society] [Engineering in Medicine and Biology, 2002
We report a slide based microarray platform for assaying multiple enzyme activities using fluorogenic substrates. The method enables us to achieve the microfluidic requirements for rapid reaction assembly and compartmentalization. We can thus determine enzymatic activities in individually controlled reaction environments containing cofactors ...
D.N. Gosalia, S.L. Diamond
openaire   +2 more sources

Two rapid assays for screening of patulin biodegradation [PDF]

, 2013
Artículo sobre distintos ensayos para comprobar la biodegradación de la patulinaThe mycotoxin patulin is produced by the blue mould pathogen Penicillium expansum in rotting apples during postharvest storage.
A Ciegler, A Ciegler, A Halász, A Ricelli, AB Gonçalves, EE Stinson, ER McKinley, G Hageskal, G Hageskal, G Lima, IE Yates, J Acar, J Harwig, J He, J Reiss, K Gromadzka, M Vansteelandt, MM Moake, MO Moss, MS Anderson, N Pérez-Guerra, NB Sammon, P Karlovsky, PM Scott, QK Wu, R Castoria, R Castoria, R Castoria, R Castoria, R Castoria, R Fliege, R Steiman, S MacDonald, SAI Wright, T Robinson, VG Lilly, VJ Pereira, VM Siqueira, ZL Sumbu   +38 more
core   +3 more sources

Dihydroorotate dehydrogenase (DHODH) regulates trophoblast syncytialization through organelle stress–induced cellular senescence

FEBS Open Bio, EarlyView.
The inhibition of mitochondrial dihydroorotate dehydrogenase (DHODH) impairs syncytialization and induces cellular senescence via mitochondrial and endoplasmic reticulum stress in human trophoblast stem cells, elevating sFlt1/PlGF levels, a hallmark of placental dysfunction in hypertensive disorders of pregnancy.
Kanoko Yoshida, Kazuya Kusama, Ayaka Horiuchi, Yu Kawaguchi, Atsuya Tsuru, Mikihiro Yoshie, Kazuhiro Tamura   +6 more
wiley   +1 more source