Results 71 to 80 of about 231,120 (294)
A Rapid Transglutaminase Assay for High-Throughput Screening Applications [PDF]
SLAS Discovery, 2006 Transglutaminases (TGs) are widely distributed enzymes that catalyze posttranslational modification of proteins by Ca(2+)-dependent cross-linking reactions. The family members of TGs participate in many significant processes of biological functions such as tissue regeneration, cell differentiation, apoptosis, and certain pathologies.
Yu-Wei, Wu, Yu-Hui, Tsai
openaire +2 more sources
Development and optimization of a miniaturized western blot-based screening platform to identify regulators of post-translational modifications [PDF]
, 2019 Post-translational modifications (PTMs) are fundamental traits of protein functionality and their study has been addressed using several approaches over the past years.
Gottifredi, Vanesa +2 more
core +1 more source
Diversity and complexity in neural organoids
FEBS Letters, EarlyView.Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley +1 more source
A high-throughput screening assay for pyruvate carboxylase
Analytical Biochemistry, 2018 Pyruvate carboxylase (PC) catalyzes the conversion of pyruvate to oxaloacetate (OAA), an important metabolic reaction in a wide range of organisms. Small molecules directed against PC would enable detailed studies on the metabolic role of this enzyme and would have the potential to be developed into pharmacological agents.
Brittney N. Wyatt +2 more
openaire +3 more sources
Optical mapping of neuronal activity during seizures in zebrafish [PDF]
, 2017 Mapping neuronal activity during the onset and propagation of epileptic seizures can provide a better understanding of the mechanisms underlying this pathology and improve our approaches to the development of new drugs.
Fornetto, C +10 more
core +3 more sources
Molecular Oncology, EarlyView.Combining PTEN protein assessment and transcriptomic profiling of prostate tumors, we uncovered a network enriched in senescence and extracellular matrix (ECM) programs associated with PTEN loss and conserved in a mouse model. We show that PTEN‐deficient cells trigger paracrine remodeling of the surrounding stroma and this information could help ...
Ivana Rondon‐Lorefice +16 more
wiley +1 more source
Communications Biology, 2022 High-throughput screening of 120,000 compounds followed by counter-screening and validation assays reveals candidate antimalarial drugs that kill Plasmodium falciparum sexual and asexual blood stages blocking parasite transmission through mosquito.
Giacomo Paonessa +13 more
doaj +1 more source
Molecular Oncology, EarlyView.Tumors contain diverse cellular states whose behavior is shaped by context‐dependent gene coordination. By comparing gene–gene relationships across biological contexts, we identify adaptive transcriptional modules that reorganize into distinct vulnerability axes.
Brian Nelson +9 more
wiley +1 more source
STAR Protocols, 2022 Summary: The Kinetic Intra-Cellular Assay (KICA) is a recombinant cell-based technique that utilizes NanoBRET technology. KICA enables the measurement of intracellular binding kinetics.
Charles S. Lay +5 more
doaj +1 more source
Development of High-Throughput Screening Assay for Antihantaviral Therapeutics [PDF]
SLAS Discovery, 2017 Humans acquire hantavirus infection by the inhalation of aerosolized excreta of infected rodent hosts. There is no treatment for hantavirus diseases at present. Therapeutic intervention during early stages of viral infection can improve the outcome of this zoonotic viral illness.
Roy, Anuradha, Mir, Mohammad A.
openaire +3 more sources