Results 71 to 80 of about 137,634 (294)

Heterozygous loss‐of‐function alleles associate the conserved 3′‐5′ exoribonuclease EXOSC10 with hypersensitivity to the anticancer drug 5‐fluorouracil

Molecular Oncology, EarlyView.
EXOSC10, an essential nuclear RNA exosome‐associated 3′‐5′ exoribonuclease, is inhibited by the anticancer drug 5‐fluorouracil (5‐FU), and EXOSC10 depletion increases 5‐FU sensitivity. The colon‐cancer variant EXOSC10S402T, located in a proteolysis motif, is stable and nuclear but nonfunctional in vivo.
Radhika Sain, Yann Le Page, Frédéric Percevault, Emmanuelle Becker, Luc Negroni, Almudena Fernandez, Marta Cantero, Diego Muñoz‐Santos, Lluís Montoliu, Cyrille Garnier, Michael Primig   +10 more
wiley   +1 more source

A high-throughput screening assay of ascorbate in brain samples [PDF]

Journal of Neuroscience Methods, 2011
Ascorbate is a vital reductant/free radical scavenger in the CNS, whose content defines - to a large extent - the redox status and the antioxidant reserves. Quick, reliable and specific methods for its measurement in brain samples are highly desirable.
Natalia A, Belikova, Ashley L, Glumac, Valentyna, Kapralova, Amin, Cheikhi, Yulia Y, Tyurina, Vincent A, Vagni, Patrick M, Kochanek, Valerian E, Kagan, Hülya, Bayir   +8 more
openaire   +2 more sources

A kinetic intra-cellular assay (KICA) to measure quantitative compound binding kinetics within living cells

STAR Protocols, 2022
Summary: The Kinetic Intra-Cellular Assay (KICA) is a recombinant cell-based technique that utilizes NanoBRET technology. KICA enables the measurement of intracellular binding kinetics.
Charles S. Lay, Daniel A. Thomas, John P. Evans, Emma J. Jones, Kelly M. Gatfield, Peter D. Craggs   +5 more
doaj   +1 more source

Adaptation of Aequorin Functional Assay to High Throughput Screening [PDF]

SLAS Discovery, 2002
AequoScreen, a cellular aequorin-based functional assay, has been optimized for luminescent high-throughput screening (HTS) of G protein-coupled receptor (GPCRs). AequoScreen is a homogeneous assay in which the cells are loaded with the apoaequorin cofactor coelenterazine, diluted in assay buffer, and injected into plates containing the samples to be ...
Emmanuel, Le Poul, Sunao, Hisada, Yoshinori, Mizuguchi, Vincent J, Dupriez, Emmanuel, Burgeon, Michel, Detheux   +5 more
openaire   +2 more sources

Cell‐cycle‐specific lesion evolution rather than inhibition of double‐strand‐break repair underpins cisplatin radiosensitization

Molecular Oncology, EarlyView.
We analyze cisplatin–DNA adducts (CDAs) and double‐strand breaks (DSBs) in a cell‐cycle‐dependent manner. We find that CDAs form similarly across all cell cycle phases. DSBs arise only in S‐phase. CDAs might not directly impair DSB repair, but S‐phase DSB lesions evolve in the presence of CDAs and disrupt repair in G2, also causing radiosensitization ...
Ye Qiu, Xixi Lin, Emil Mladenov, Veronika Mladenova, Jürgen Thomale, Ali Sak, Yao Wang, Yunxuan Deng, Eleni Gkika, Martin Stuschke, George Iliakis   +10 more
wiley   +1 more source

Longitudinal circulating tumor DNA profiling in patients with advanced endometrial cancer using an off‐the‐shelf targeted NGS panel

Molecular Oncology, EarlyView.
Intratumour heterogeneity complicates precision management of advanced endometrial cancer. Circulating tumor DNA (ctDNA) offers a minimally invasive strategy to capture tumor evolution and therapeutic resistance. Here, we compare tumor‐agnostic NGS with tumor‐informed ddPCR, outlining their relative sensitivity, concordance, and clinical implications ...
Carlos Casas‐Arozamena, Karin Teien Lande, Eva Diaz, Ana Vilar, Juan Cueva, Efigenia Arias, Victoria Sampayo, Alicia Abalo, Nerea González, Eva Colás, Antonio Gil‐Moreno, Miguel Abal, Gema Moreno‐Bueno, Therese Sørlie, Kristina Lindemann, Laura Muinelo‐Romay   +15 more
wiley   +1 more source

Application of RNAi to Genomic Drug Target Validation in Schistosomes. [PDF]

, 2015
Concerns over the possibility of resistance developing to praziquantel (PZQ), has stimulated efforts to develop new drugs for schistosomiasis. In addition to the development of improved whole organism screens, the success of RNA interference (RNAi) in ...
Bickle, Quentin D, Bickle, Quentin D., Carruthers, Ian M, Gilbert, Ian H, Ian H Gilbert, Nuha R. Mansour (146895), Andrew L. Hopkins (146939), Jérémy Besnard (741480), Hopkins, Andrew L, Gilbert, Ian H., Guidi, Alessandra, Quentin D. Bickle (146947), Ian H. Gilbert (146934), Alessandra Guidi (146927), Mansour, Nuha R., Paveley, Ross A., Alessandra Guidi, Ross A Paveley, Ian M Carruthers, Carruthers, Ian M., Jérémy Besnard, Quentin D Bickle, Paveley, Ross A, Besnard, Jérémy, Nuha R Mansour, Ross A. Paveley (146889), Mansour, Nuha R, Hopkins, Andrew L., Andrew L Hopkins, Ian M. Carruthers (741479)   +29 more
core   +1 more source

Interaction of HS1BP3 with cortactin modulates TKS5 localisation, cell secretion and cancer malignancy

Molecular Oncology, EarlyView.
Here, we demonstrate that HS1BP3 interacts with Cortactin through a proline‐rich region (PRR3.1) and show that this interaction, and HS1BP3 itself, promote cancer cell proliferation and invasion. Inhibition of this interaction leads to build‐up of TKS5 in multivesicular endosomes and altered secretion of CD63 and CD9, providing an explanation for the ...
Arja Arnesen Løchen, Kristiane Søreng, Chiara Veroni, Laura Trachsel‐Moncho, Nagham Asp, Robin Gaupset, Lars Gustav Lyckander, Helene Knævelsrud, Lars Eftang, Anne Simonsen   +9 more
wiley   +1 more source

Code for "DRABAL: novel method to mine large high-throughput screening assays using Bayesian active learning"

, 2016
Background Mining high-throughput screening (HTS) assays is key for enhancing decisions in the area of drug repositioning and drug discovery. However, many challenges are encountered in the process of developing suitable and accurate methods for ...
Soufan, Othman, Ba Alawi, Wail, Bajic, Vladimir B., Afeef, Moataz A., Kalnis, Panos, Essack, Magbubah   +5 more
core   +1 more source

Validation of a modified fluorimetric assay for the screening of trichomonacidal drugs

Memorias do Instituto Oswaldo Cruz, 2012
A fluorimetric microassay that uses a redox dye to determine the viability of the flagellate Trichomonas vaginalis has been optimised to provide a more sensitive method to evaluate potential trichomonacidal compounds.
Alexandra Ibáñez Escribano, Alfredo Meneses Marcel, Yanetsy Machado Tugores, Juan José Nogal Ruiz, Vicente J Arán Redó, José Antonio Escario García-Trevijano, Alicia Gómez Barrio   +6 more
doaj   +1 more source