Results 51 to 60 of about 16,128,167 (280)
Diversity and complexity in neural organoids
FEBS Letters, EarlyView.Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley +1 more source
STAR ProtocolsSummary: The interaction between cell surface receptors and their ligands is crucial for intercellular communication. However, current techniques for identifying direct receptor-ligand interactions remain limited.
Xiaoli Hu, Yiguo Wang
doaj +1 more source
Organoid drug screening in cancer: advances in high-throughput platforms and clinical applications [PDF]
OrganoidPatient-derived tumor organoids represent the most physiologically relevant 3-dimensional (3D) cancer models, preserving tumor heterogeneity and native tissue architecture.
Jiho Lee, Hyun Ju Kang, Jin-Ku Lee
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High-throughput drug screening of fine-needle aspiration-derived cancer organoids
STAR Protocols, 2020 Summary: Generation of fine-needle aspiration (FNA)-derived cancer organoids has allowed us to develop a number of downstream applications. In this protocol, we start with organoids cultured in a semi-solid format.
Kensey Bergdorf +7 more
doaj +1 more source
A system for performing high throughput assays of synaptic function. [PDF]
PLoS ONE, 2011 Unbiased, high-throughput screening has proven invaluable for dissecting complex biological processes. Application of this general approach to synaptic function would have a major impact on neuroscience research and drug discovery.
Chris M Hempel +9 more
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Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential
FEBS Letters, EarlyView.Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta +3 more
wiley +1 more source
High-throughput screening assay used in pharmacognosy: Selection, optimization and validation of methods of enzymatic inhibition by UV-visible spectrophotometry [PDF]
Journal of Pharmacy & Pharmacognosy Research, 2014 In research laboratories of both organic synthesis and extraction of natural products, every day a lot of products that can potentially introduce some biological activity are obtained.
Graciela Granados-Guzmán +3 more
doaj
Journal of Chemistry, 2021 The effects of reaction conditions on the spectrophotometric and fluorometric assays using alternative substrates (p-nitrophenyl palmitate and 4-methylumbelliferyl oleate) were investigated to optimize them for the high-throughput screening of lipase ...
Jun-Young Park +4 more
doaj +1 more source
Structure‐forward targeting of claudins with synthetic binders
FEBS Letters, EarlyView.Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley +1 more source
High-throughput mass spectrometry based assays for detection of reactive compounds
Green Analytical ChemistryHigh-throughput screening (HTS) is a foundational approach in small molecule drug discovery, but false positives due to reactive compounds can waste resources and hinder progress. Nuisance assays are therefore required to identify such compounds; however,
Fan Pu +7 more
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