Results 51 to 60 of about 231,120 (294)

A system for performing high throughput assays of synaptic function. [PDF]

PLoS ONE, 2011
Unbiased, high-throughput screening has proven invaluable for dissecting complex biological processes. Application of this general approach to synaptic function would have a major impact on neuroscience research and drug discovery.
Chris M Hempel, Michael Sivula, Jonathan M Levenson, David M Rose, Bing Li, Ana C Sirianni, Eva Xia, Timothy A Ryan, David J Gerber, Jeffrey R Cottrell   +9 more
doaj   +1 more source

Organoids in pediatric cancer research

FEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source

Protocol to identify small-molecule inhibitors against cancer drug resistance

STAR Protocols
Summary: Drug resistance has emerged as a critical challenge in clinical cancer treatment. Here, we present a high-throughput screening protocol to identify therapeutic small-molecule inhibitors against drug-resistant cancer cells.
Juan Wei, She-Yu Zhang, Xue-Yuan Zhou, Yong Wei, Hao-Ran Jia, Qin Wu, Weihong Tan   +6 more
doaj   +1 more source

Development of a High Throughput Platform for Screening Glycoside Hydrolases based on Oxime-NIMS

Frontiers in Bioengineering and Biotechnology, 2015
Cost effective hydrolysis of biomass into sugars for biofuel production requires high-performance low-cost glycoside hydrolase (GH) cocktails that are active under demanding process conditions.
Kai eDeng, Kai eDeng, Joel M Guenther, Joel M Guenther, Jian eGao, Benjamin Paul Bowen, Huu eTran, Huu eTran, Vimalier eReyes-Ortiz, Vimalier eReyes-Ortiz, Xiaoliang eCheng, Xiaoliang eCheng, Noppadon eSathitsuksanoh, Noppadon eSathitsuksanoh, Richard eHeins, Richard eHeins, Taichi E Takasuka, Lai eBergeman, Henrik eGeertz-Hansen, Henrik eGeertz-Hansen, Sam eDeutsch, Sam eDeutsch, Dominique eLoqué, Dominique eLoqué, Kenneth eSale, Kenneth eSale, Blake eSimmons, Blake eSimmons, Paul D Adam, Paul D Adam, Paul D Adam, Anup K Singh, Anup K Singh, Brian Grant Fox, Brian Grant Fox, Trent Russell Northen, Trent Russell Northen   +36 more
doaj   +1 more source

Docking-Based Virtual Screening Enables Prioritizing Protein Kinase Inhibitors With In Vitro Phenotypic Activity Against Schistosoma mansoni

Frontiers in Cellular and Infection Microbiology, 2022
Schistosomiasis is a parasitic neglected disease with praziquantel (PZQ) utilized as the main drug for treatment, despite its low effectiveness against early stages of the worm. To aid in the search for new drugs to tackle schistosomiasis, computer-aided
Bernardo Pereira Moreira, Izabella Cristina Andrade Batista, Naiara Clemente Tavares, Tom Armstrong, Sandra Grossi Gava, Gabriella Parreiras Torres, Marina Moraes Mourão, Franco H. Falcone   +7 more
doaj   +1 more source

The newfound relationship between extrachromosomal DNAs and excised signal circles

FEBS Letters, EarlyView.
Extrachromosomal DNAs (ecDNAs) contribute to the progression of many human cancers. In addition, circular DNA by‐products of V(D)J recombination, excised signal circles (ESCs), have roles in cancer progression but have largely been overlooked. In this Review, we explore the roles of ecDNAs and ESCs in cancer development, and highlight why these ...
Dylan Casey, Zeqian Gao, Joan Boyes
wiley   +1 more source

Protocol for screening inhibitors of the fungal sphingolipid-metabolizing enzyme ceramide synthase

STAR Protocols
Summary: Fungal sphingolipids have emerged as potent antifungal targets based on cumulative evidence demonstrating that fungal sphingolipid metabolism is essential to the virulence of pathogenic fungi. Here, we present a protocol to screen for inhibitors
Andy Zhong, Deveney Dasilva, Nivea Pereira de Sa, Jeehyun Karen You, Maurizio Del Poeta   +4 more
doaj   +1 more source

High-throughput drug screening of fine-needle aspiration-derived cancer organoids

STAR Protocols, 2020
Summary: Generation of fine-needle aspiration (FNA)-derived cancer organoids has allowed us to develop a number of downstream applications. In this protocol, we start with organoids cultured in a semi-solid format.
Kensey Bergdorf, Courtney Phifer, Vijaya Bharti, David Westover, Joshua Bauer, Anna Vilgelm, Ethan Lee, Vivian Weiss   +7 more
doaj   +1 more source

Robust Analysis of High Throughput Screening (HTS) Assay Data [PDF]

Technometrics, 2013
Quantitative high throughput screening (qHTS) assays use cells or tissues to screen thousands of compounds in a short period of time. Data generated from qHTS assays are then evaluated using nonlinear regression models, such as the Hill model, and decisions regarding toxicity are made using the estimates of the parameters of the model.
Changwon, Lim, Pranab K, Sen, Shyamal D, Peddada   +2 more
openaire   +2 more sources

Cell wall target fragment discovery using a low‐cost, minimal fragment library

FEBS Letters, EarlyView.
LoCoFrag100 is a fragment library made up of 100 different compounds. Similarity between the fragments is minimized and 10 different fragments are mixed into a single cocktail, which is soaked to protein crystals. These crystals are analysed by X‐ray crystallography, revealing the binding modes of the bound fragment ligands.
Kaizhou Yan, Mathew Stanley, Olawale Raimi, Andrew T. Ferenbach, Helge C Dorfmueller, Daan M. F. van Aalten   +5 more
wiley   +1 more source