Results 31 to 40 of about 137,634 (294)
A high-throughput screening assay for pyruvate carboxylase
Analytical Biochemistry, 2018 Pyruvate carboxylase (PC) catalyzes the conversion of pyruvate to oxaloacetate (OAA), an important metabolic reaction in a wide range of organisms. Small molecules directed against PC would enable detailed studies on the metabolic role of this enzyme and would have the potential to be developed into pharmacological agents.
Brittney N. Wyatt +2 more
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High-Throughput Screening to Predict Chemical-Assay Interference [PDF]
Scientific Reports, 2020 AbstractThe U.S. federal consortium on toxicology in the 21st century (Tox21) produces quantitative, high-throughput screening (HTS) data on thousands of chemicals across a wide range of assays covering critical biological targets and cellular pathways.
Alexandre Borrel +8 more
openaire +2 more sources
Advances in the application of high-throughput microfluidic platforms in precise diagnosis and treatment of tumors [PDF]
精准医学杂志Although the treatment of malignant tumors has entered an era of comprehensive therapy integrating chemotherapy, targeted therapy, immunotherapy, and other modalities, there is still a significant gap from individualized precise treatment.
DOU Liliang, CHU Jiahui, LIU Lian
doaj +1 more source
Methods to identify the unexplored diversity of microbial exopolysaccharides
Frontiers in Microbiology, 2015 Microbial exopolysaccharides (EPS) are a structurally very diverse class of molecules. A number of them have found their application in rather diverging fields that extend from medicine, food and cosmetics on the one side to construction, drilling and ...
Broder eRühmann +2 more
doaj +1 more source
Protocol for tissue slice cultures from human solid tumors to study therapeutic response
STAR Protocols, 2021 Summary: The impact of systemic therapy on the tumor microenvironment has been difficult to study in human solid tumors. Our protocol describes steps for establishing slice cultures to investigate response to chemotherapies, immunotherapies, or adoptive ...
Heidi L. Kenerson +4 more
doaj +1 more source
STAR Protocols, 2021 Summary: Here, we describe an optimized protocol to identify specific nuclear receptor ligands. First, to rule out any compound interference with luciferase activity per se, we describe an in vitro assay assessing potential inhibition or activation of ...
Alexandre Berthier +2 more
doaj +1 more source
High Throughput Centrifugal Adhesion Screening Test for Soft Materials
, 2021 High-throughput screening of material mechanical properties has the potential to transform material science research in both aiding in material discovery and developing predictive models.
Kenneth, Shull +6 more
core +1 more source
Quantify permeability using on-a-chip models in high-throughput applications
STAR Protocols, 2023 Summary: Traditionally, to quantify permeability of a biological barrier, the initial slope is used, based on the assumption of sink condition (concentration of the donor is constant, and the receiver increases less than 10%).
Camilla Soragni +7 more
doaj +1 more source
Assay in high throughput screening
Folia Pharmacologica Japonica, 2001 HTS (High Throughput Screening) has been put into practice in recent years. HTS is aiming to discover lead compounds for medicinal drugs. High efficiency must be achieved in all the processes including sample preparation, assay procedure, automation and data management.
openaire +3 more sources
A Rapid Transglutaminase Assay for High-Throughput Screening Applications [PDF]
SLAS Discovery, 2006 Transglutaminases (TGs) are widely distributed enzymes that catalyze posttranslational modification of proteins by Ca(2+)-dependent cross-linking reactions. The family members of TGs participate in many significant processes of biological functions such as tissue regeneration, cell differentiation, apoptosis, and certain pathologies.
Yu-Wei, Wu, Yu-Hui, Tsai
openaire +2 more sources