Frontiers in Microbiology, 2023 Neisseria gonorrhoeae (gonococcus) is an obligate human pathogen and the etiological agent of the sexually transmitted disease gonorrhea. The rapid rise in gonococcal resistance to all currently available antimicrobials has become a significant public ...
Samuele Stazzoni +7 more
doaj +1 more source
A novel FRET-based screen in high-throughput format to identify inhibitors of malarial and human glucose transporters [PDF]
, 2016 The glucose transporter PfHT is essential to the survival of the malaria parasite Plasmodium falciparum and has been shown to be a druggable target with high potential for pharmacological intervention.
Autry, Joseph M +9 more
core +2 more sources
Enhanced yeast one-hybrid screens to identify transcription factor binding to human DNA sequences [PDF]
, 2019 Identifying the sets of transcription factors (TFs) that regulate each human gene is a daunting task that requires integrating numerous experimental and computational approaches.
Fuxman Bass, Juan +3 more
core +1 more source
RyR1-targeted drug discovery pipeline integrating FRET-based high-throughput screening and human myofiber dynamic Ca2+ assays. [PDF]
, 2020 Elevated cytoplasmic [Ca2+] is characteristic in severe skeletal and cardiac myopathies, diabetes, and neurodegeneration, and partly results from increased Ca2+ leak from sarcoplasmic reticulum stores via dysregulated ryanodine receptor (RyR) channels ...
Bers, Donald M +6 more
core +2 more sources
Rational methods for the selection of diverse screening compounds. [PDF]
, 2011 Traditionally a pursuit of large pharmaceutical companies, high-throughput screening assays are becoming increasingly common within academic and government laboratories.
Huggins, David J +2 more
core +2 more sources
Advances in the application of high-throughput microfluidic platforms in precise diagnosis and treatment of tumors [PDF]
精准医学杂志Although the treatment of malignant tumors has entered an era of comprehensive therapy integrating chemotherapy, targeted therapy, immunotherapy, and other modalities, there is still a significant gap from individualized precise treatment.
DOU Liliang, CHU Jiahui, LIU Lian
doaj +1 more source
Multi-Behavioral Endpoint Testing Of An 87-Chemical Compound Library In Freshwater Planarians [PDF]
, 2018 There is an increased recognition in the field of toxicology of the value of medium-to-high-throughput screening methods using in vitro and alternative animal models.
Collins, Eva-Maria S. +4 more
core +3 more sources
Methods to identify the unexplored diversity of microbial exopolysaccharides
Frontiers in Microbiology, 2015 Microbial exopolysaccharides (EPS) are a structurally very diverse class of molecules. A number of them have found their application in rather diverging fields that extend from medicine, food and cosmetics on the one side to construction, drilling and ...
Broder eRühmann +2 more
doaj +1 more source
Protocol for tissue slice cultures from human solid tumors to study therapeutic response
STAR Protocols, 2021 Summary: The impact of systemic therapy on the tumor microenvironment has been difficult to study in human solid tumors. Our protocol describes steps for establishing slice cultures to investigate response to chemotherapies, immunotherapies, or adoptive ...
Heidi L. Kenerson +4 more
doaj +1 more source
STAR Protocols, 2021 Summary: Here, we describe an optimized protocol to identify specific nuclear receptor ligands. First, to rule out any compound interference with luciferase activity per se, we describe an in vitro assay assessing potential inhibition or activation of ...
Alexandre Berthier +2 more
doaj +1 more source