Results 31 to 40 of about 123,864 (254)
Frontiers in Microbiology, 2016 Nicotinamidases catalyze the hydrolysis of the amide bond in nicotinamide to produce ammonia and nicotinic acid. These enzymes are an essential component of the NAD+ salvage pathway and are implicated in the viability of several pathogenic organisms. Its
Rubén Zapata-Pérez +4 more
doaj +1 more source
Quantification of drug-induced fractional killing using high-throughput microscopy
STAR Protocols, 2021 Summary: Anti-cancer drugs kill only a fraction of cells within a population at any given time. Here, we describe a protocol to quantify drug-induced fractional killing over time using high-throughput imaging.
Zintis Inde +2 more
doaj +1 more source
STAR Protocols, 2022 Summary: Well-characterized small molecules enable the study of cell processes and facilitate target validation. Here, we describe a high-content multiplex screen to investigate cell viability over 48 h, which can be combined with investigating ...
Amelie Tjaden +4 more
doaj +1 more source
A Rapid Transglutaminase Assay for High-Throughput Screening Applications [PDF]
SLAS Discovery, 2006 Transglutaminases (TGs) are widely distributed enzymes that catalyze posttranslational modification of proteins by Ca(2+)-dependent cross-linking reactions. The family members of TGs participate in many significant processes of biological functions such as tissue regeneration, cell differentiation, apoptosis, and certain pathologies.
Yu-Wei, Wu, Yu-Hui, Tsai
openaire +2 more sources
Arbitrarily Accessible 3D Microfluidic Device for Combinatorial High-Throughput Drug Screening
Sensors, 2016 Microfluidics-based drug-screening systems have enabled efficient and high-throughput drug screening, but their routine uses in ordinary labs are limited due to the complexity involved in device fabrication and system setup.
Zhuofa Chen +6 more
doaj +1 more source
Development of a High Throughput Platform for Screening Glycoside Hydrolases based on Oxime-NIMS
Frontiers in Bioengineering and Biotechnology, 2015 Cost effective hydrolysis of biomass into sugars for biofuel production requires high-performance low-cost glycoside hydrolase (GH) cocktails that are active under demanding process conditions.
Kai eDeng +36 more
doaj +1 more source
Organoids in pediatric cancer research
FEBS Letters, EarlyView.Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley +1 more source
Protocol to identify small-molecule inhibitors against cancer drug resistance
STAR ProtocolsSummary: Drug resistance has emerged as a critical challenge in clinical cancer treatment. Here, we present a high-throughput screening protocol to identify therapeutic small-molecule inhibitors against drug-resistant cancer cells.
Juan Wei +6 more
doaj +1 more source
Frontiers in Cellular and Infection Microbiology, 2022 Schistosomiasis is a parasitic neglected disease with praziquantel (PZQ) utilized as the main drug for treatment, despite its low effectiveness against early stages of the worm. To aid in the search for new drugs to tackle schistosomiasis, computer-aided
Bernardo Pereira Moreira +7 more
doaj +1 more source
FEBS Letters, EarlyView.We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee +3 more
wiley +1 more source