Results 31 to 40 of about 16,128,167 (280)

An optimized protocol with a stepwise approach to identify specific nuclear receptor ligands from cultured mammalian cells

STAR Protocols, 2021
Summary: Here, we describe an optimized protocol to identify specific nuclear receptor ligands. First, to rule out any compound interference with luciferase activity per se, we describe an in vitro assay assessing potential inhibition or activation of ...
Alexandre Berthier, Bart Staels, Philippe Lefebvre   +2 more
doaj   +1 more source

Quantify permeability using on-a-chip models in high-throughput applications

STAR Protocols, 2023
Summary: Traditionally, to quantify permeability of a biological barrier, the initial slope is used, based on the assumption of sink condition (concentration of the donor is constant, and the receiver increases less than 10%).
Camilla Soragni, Tessa Vergroesen, Nynke Hettema, Gwenaëlle Rabussier, Henriëtte L. Lanz, Sebastian J. Trietsch, Leon J. de Windt, Chee P. Ng   +7 more
doaj   +1 more source

Assay in high throughput screening

Folia Pharmacologica Japonica, 2001
HTS (High Throughput Screening) has been put into practice in recent years. HTS is aiming to discover lead compounds for medicinal drugs. High efficiency must be achieved in all the processes including sample preparation, assay procedure, automation and data management.
openaire   +3 more sources

Combined whole-cell high-throughput functional screening for identification of new nicotinamidases/pyrazinamidases in metagenomic/polygenomic libraries

Frontiers in Microbiology, 2016
Nicotinamidases catalyze the hydrolysis of the amide bond in nicotinamide to produce ammonia and nicotinic acid. These enzymes are an essential component of the NAD+ salvage pathway and are implicated in the viability of several pathogenic organisms. Its
Rubén Zapata-Pérez, Antonio Ginés García-Saura, Mohamed Jebbar, Peter Golyshin, Álvaro Sánchez-Ferrer   +4 more
doaj   +1 more source

Quantification of drug-induced fractional killing using high-throughput microscopy

STAR Protocols, 2021
Summary: Anti-cancer drugs kill only a fraction of cells within a population at any given time. Here, we describe a protocol to quantify drug-induced fractional killing over time using high-throughput imaging.
Zintis Inde, Jason Rodencal, Scott J. Dixon   +2 more
doaj   +1 more source

High throughput screens yield small molecule inhibitors of Leishmania CRK3:CYC6 cyclin-dependent kinase [PDF]

, 2011
<p><b>Background:</b> <i>Leishmania</i> species are parasitic protozoa that have a tightly controlled cell cycle, regulated by cyclin-dependent kinases (CDKs).
Mottram Jeremy C., Thomson, Graeme, Mottram, JC, Grant Karen M., Turner, N.J., Malcolm D. Walkinshaw, Malone, Kirk, Turner, Nicholas J., Nicholas J Turner, Jeremy C. Mottram, Karen M. Grant, Walker, Roderick G., Kita, K, Graeme Thomson, Malone Kirk, Karen M Grant, Grant, Karen, Nicholas J. Turner, Walker, RG, Nowicki, Matthew W., Thomson Graeme, Walkinshaw, M.D., Walkinshaw Malcolm D., David G. Blake, David G Blake, Walker Roderick G., Brown, Elaine, Brown Elaine, Blake David G., Brown, E, Malone, K, Turner Nicholas J., Walkinshaw, MD, Blake, DG, Matthew W. Nowicki, Walker, R.G., Malone, K., Blake, David G., Elaine Brown, Mottram, J.C., Kirk Malone, Walkinshaw, Malcolm D., Matthew W Nowicki, Nowicki, MW, Brown, E., Nowicki Matthew W., Turner, Nicholas J.; id_orcid, Grant, Karen M., Grant, K.M., Mottram, Jeremy C., Roderick G Walker, Thomson, G, Jeremy C Mottram, Roderick G. Walker, Nowicki, M.W., Grant, KM, Blake, D.G., Malcolm D Walkinshaw, Turner, NJ, Thomson, G.   +59 more
core   +2 more sources

High Throughput Centrifugal Adhesion Screening Test for Soft Materials [PDF]

, 2021
High-throughput screening of material mechanical properties has the potential to transform material science research in both aiding in material discovery and developing predictive models.
Kenneth, Shull, Yusu, Chen, Johanna, Kann, Carolyn, Mills, Danielle, Tullman-Ercek, Muzhou, Wang, Qifeng, Wang   +6 more
core   +2 more sources

High-content live-cell multiplex screen for chemogenomic compound annotation based on nuclear morphology

STAR Protocols, 2022
Summary: Well-characterized small molecules enable the study of cell processes and facilitate target validation. Here, we describe a high-content multiplex screen to investigate cell viability over 48 h, which can be combined with investigating ...
Amelie Tjaden, Robert T. Giessmann, Stefan Knapp, Martin Schröder, Susanne Müller   +4 more
doaj   +1 more source

A high-throughput screening assay for pyruvate carboxylase

Analytical Biochemistry, 2018
Pyruvate carboxylase (PC) catalyzes the conversion of pyruvate to oxaloacetate (OAA), an important metabolic reaction in a wide range of organisms. Small molecules directed against PC would enable detailed studies on the metabolic role of this enzyme and would have the potential to be developed into pharmacological agents.
Brittney N. Wyatt, Leggy A. Arnold, Martin St. Maurice   +2 more
openaire   +3 more sources

Fluorescence/luminescence-based markers for the assessment of Schistosoma mansoni schistosomula drug assays [PDF]

, 2015
Schistosomiasis is responsible for a tremendous public health burden, yet only a single drug, praziquantel, is available. New antischistosomal treatments should therefore be developed. The accuracy, speed and objectivity of in vitro drug screening depend
Gordana Panic, Jennifer Keiser, Flores, Dayana, Keiser, Jennifer, Ingram-Sieber, Katrin, Dayana Flores, Katrin Ingram-Sieber, Panic, Gordana   +7 more
core   +1 more source