Results 11 to 20 of about 123,864 (254)
siMacro: A Fast and Easy Data Processing Tool for Cell-Based Genomewide siRNA Screens [PDF]
Genomics & Informatics, 2013 Growing numbers of studies employ cell line-based systematic short interfering RNA (siRNA) screens to study gene functions and to identify drug targets.
Nitin Kumar Singh +4 more
doaj +1 more source
FLICK: An optimized plate reader-based assay to infer cell death kinetics
STAR Protocols, 2021 Summary: Evaluating drug sensitivity is improved by directly quantifying death kinetics, rather than correlates of viability, such as metabolic activity.
Ryan Richards +2 more
doaj +1 more source
A high-throughput SARS-CoV-2 pseudovirus multiplex neutralization assay
STAR Protocols, 2022 Summary: Evaluating the neutralizing antibody titer following SARS-CoV-2 vaccination is essential in defining correlates of protection. We describe an assay that uses single-cycle vesicular stomatitis virus (VSV) pseudoviruses linking a fluorophore with ...
Benjamin Louis Sievers +2 more
doaj +1 more source
Screening and High-Throughput Platelet Assays [PDF]
, 2018 High-throughput assays are important biological research tools but are rarely utilized for platelet research. However, screening compounds for efficacy against a physiologically relevant cellular response in primary cells such as platelets can be an advantageous approach to compound screening and drug development. In this section we describe a panel of
Bye, Alexander P +2 more
openaire +3 more sources
Integrated Model of Chemical Perturbations of a Biological Pathway Using 18In VitroHigh-Throughput Screening Assays for the Estrogen Receptor [PDF]
Toxicological Sciences, 2015 , Menghang Xia, Keith Houck
exaly +2 more sources
STAR Protocols, 2021 Summary: Autophagy measurement has been challenging due to the transient nature of autophagy vesicles, in which degradation of cargo occurs. Here, we present a protocol to monitor starvation-induced autophagy using a live high-throughput microscopy ...
Ada Nowosad, Arnaud Besson
doaj +1 more source
STAR Protocols, 2023 Summary: The complement receptors C3aR and C5aR1 are promising therapeutic targets. Here, we present a protocol to screen the effects of different agonists and antagonists on these receptors in vitro, using phosphorylated extracellular signal-regulated ...
Xaria X. Li, Trent M. Woodruff
doaj +1 more source
Assessment of protein inclusions in cultured cells using automated image analysis
STAR Protocols, 2022 Summary: Proteinaceous inclusions are associated with neurodegenerative diseases and cell models are often used to determine genetic and chemical modifiers of their formation.
Luke McAlary +6 more
doaj +1 more source
Alginate-based 3D cancer cell culture for therapeutic response modeling
STAR Protocols, 2021 Summary: Two-dimensional (2D) culture of tumor cells fails to recapitulate some important aspects of cellular organization seen in in vivo experiments. In addition, cell cultures traditionally use non-physiological concentration of nutrients.
Farideh Davoudi +9 more
doaj +1 more source
Frontiers in Microbiology, 2023 Neisseria gonorrhoeae (gonococcus) is an obligate human pathogen and the etiological agent of the sexually transmitted disease gonorrhea. The rapid rise in gonococcal resistance to all currently available antimicrobials has become a significant public ...
Samuele Stazzoni +7 more
doaj +1 more source