Results 11 to 20 of about 16,128,167 (280)
Screening and High-Throughput Platelet Assays [PDF]
, 2018 High-throughput assays are important biological research tools but are rarely utilized for platelet research. However, screening compounds for efficacy against a physiologically relevant cellular response in primary cells such as platelets can be an advantageous approach to compound screening and drug development. In this section we describe a panel of
Bye, Alexander P +2 more
core +6 more sources
Spectrophotometric Enzyme Assays for High-Throughput Screening [PDF]
Food Technology and Biotechnology, 2004 This paper reviews high-throughput screening enzyme assays developed in our laboratory over the last ten years. These enzyme assays were initially developed for the purpose of discovering catalytic antibodies by screening cell culture supernatants, but ...
Jean-Louis Reymond
doaj +2 more sources
New High-Throughput Screening Assays for Biocatalysis [PDF]
CHIMIA, 2001 High-throughput screening for catalysis is a critical technology in all experiments aimed at modifying or creating enzymes by directed evolution, as well as for biodiversity mining for new catalysts.
Jean-Louis Reymond
doaj +4 more sources
Statistical Analysis of High Content Screening Data [PDF]
, 2009 High throughput screening experiments are typically used within the pharmaceutical industry for the identification and evaluation of candidate drugs. Using a high throughput screen with automated imaging platform allows a large number of compounds to be ...
Jacques, Richard
core +6 more sources
Complementary approaches to target human respiratory syncytial virus using small molecule ligands [PDF]
, 2012 In the current project, situated at the interface of chemistry and virology, complementary drug discovery approaches (high-throughput screening and structurebased drug design) have been exploited in order to discover novel small molecule inhibitors of ...
Lardeau, Charles-Hugues Claude Jean
core +6 more sources
A high-throughput SARS-CoV-2 pseudovirus multiplex neutralization assay
STAR Protocols, 2022 Summary: Evaluating the neutralizing antibody titer following SARS-CoV-2 vaccination is essential in defining correlates of protection. We describe an assay that uses single-cycle vesicular stomatitis virus (VSV) pseudoviruses linking a fluorophore with ...
Benjamin Louis Sievers +2 more
doaj +1 more source
High Throughput Screens Yield Small Molecule Inhibitors of Leishmania CRK3:CYC6 Cyclin-Dependent Kinase [PDF]
, 2011 Background: Leishmania species are parasitic protozoa that have a tightly controlled cell cycle, regulated by cyclin-dependent kinases (CDKs). Cdc2-related kinase 3 (CRK3), an essential CDK in Leishmania and functional orthologue of human CDK1, can form ...
Walker, Roderick G. +9 more
core +3 more sources
STAR Protocols, 2021 Summary: Autophagy measurement has been challenging due to the transient nature of autophagy vesicles, in which degradation of cargo occurs. Here, we present a protocol to monitor starvation-induced autophagy using a live high-throughput microscopy ...
Ada Nowosad, Arnaud Besson
doaj +1 more source
STAR Protocols, 2023 Summary: The complement receptors C3aR and C5aR1 are promising therapeutic targets. Here, we present a protocol to screen the effects of different agonists and antagonists on these receptors in vitro, using phosphorylated extracellular signal-regulated ...
Xaria X. Li, Trent M. Woodruff
doaj +1 more source
Assessment of protein inclusions in cultured cells using automated image analysis
STAR Protocols, 2022 Summary: Proteinaceous inclusions are associated with neurodegenerative diseases and cell models are often used to determine genetic and chemical modifiers of their formation.
Luke McAlary +6 more
doaj +1 more source