Apoptosis assessment in high-content and high-throughput screening assays
BioTechniques, 2021 Here the authors describe the development of AUTOptosis, an economical and rapid apoptosis monitoring method suitable for high-content and high-throughput screening assays.
Céline Rens +5 more
doaj +1 more source
siMacro: A Fast and Easy Data Processing Tool for Cell-Based Genomewide siRNA Screens [PDF]
Genomics & Informatics, 2013 Growing numbers of studies employ cell line-based systematic short interfering RNA (siRNA) screens to study gene functions and to identify drug targets.
Nitin Kumar Singh +4 more
doaj +1 more source
FLICK: An optimized plate reader-based assay to infer cell death kinetics
STAR Protocols, 2021 Summary: Evaluating drug sensitivity is improved by directly quantifying death kinetics, rather than correlates of viability, such as metabolic activity.
Ryan Richards +2 more
doaj +1 more source
A high-throughput SARS-CoV-2 pseudovirus multiplex neutralization assay
STAR Protocols, 2022 Summary: Evaluating the neutralizing antibody titer following SARS-CoV-2 vaccination is essential in defining correlates of protection. We describe an assay that uses single-cycle vesicular stomatitis virus (VSV) pseudoviruses linking a fluorophore with ...
Benjamin Louis Sievers +2 more
doaj +1 more source
Development and validation of a luminescence-based, medium-throughput assay for drug screening in Schistosoma mansoni [PDF]
, 2015 Schistosomiasis, one of the world's greatest neglected tropical diseases, is responsible for over 280,000 human deaths per annum. Praziquantel, developed in the 1970s, has high efficacy, excellent tolerability, few and transient side effects, simple ...
Altamura, Sergio +5 more
core +9 more sources
Screening and High-Throughput Platelet Assays [PDF]
, 2018 High-throughput assays are important biological research tools but are rarely utilized for platelet research. However, screening compounds for efficacy against a physiologically relevant cellular response in primary cells such as platelets can be an advantageous approach to compound screening and drug development. In this section we describe a panel of
Bye, Alexander P +2 more
openaire +3 more sources
High-Throughput Screening to Predict Chemical-Assay Interference [PDF]
Scientific Reports, 2020 AbstractThe U.S. federal consortium on toxicology in the 21st century (Tox21) produces quantitative, high-throughput screening (HTS) data on thousands of chemicals across a wide range of assays covering critical biological targets and cellular pathways.
Alexandre Borrel +8 more
openaire +2 more sources
New compound sets identified from high throughput phenotypic screening against three kinetoplastid parasites:an open resource [PDF]
, 2015 Using whole-cell phenotypic assays, the GlaxoSmithKline high-throughput screening (HTS) diversity set of 1.8 million compounds was screened against the three kinetoplastids most relevant to human disease, i.e.
Alonso-Padilla, Julio +20 more
core +3 more sources
An image analysis toolbox for high-throughput C. elegans assays [PDF]
, 2011 We present a toolbox for high-throughput screening of image-based Caenorhabditis elegans phenotypes. The image analysis algorithms measure morphological phenotypes in individual worms and are effective for a variety of assays and imaging systems.
AE Carpenter +35 more
core +1 more source
STAR Protocols, 2021 Summary: Autophagy measurement has been challenging due to the transient nature of autophagy vesicles, in which degradation of cargo occurs. Here, we present a protocol to monitor starvation-induced autophagy using a live high-throughput microscopy ...
Ada Nowosad, Arnaud Besson
doaj +1 more source