Results 11 to 20 of about 137,634 (294)

Screening and High-Throughput Platelet Assays [PDF]

, 2018
High-throughput assays are important biological research tools but are rarely utilized for platelet research. However, screening compounds for efficacy against a physiologically relevant cellular response in primary cells such as platelets can be an advantageous approach to compound screening and drug development. In this section we describe a panel of
Bye, Alexander P, Unsworth, Amanda J, Gibbins, Jonathan M   +2 more
openaire   +4 more sources

New High-Throughput Screening Assays for Biocatalysis

CHIMIA, 2001
High-throughput screening for catalysis is a critical technology in all experiments aimed at modifying or creating enzymes by directed evolution, as well as for biodiversity mining for new catalysts.
Jean-Louis Reymond
doaj   +5 more sources

Spectrophotometric Enzyme Assays for High-Throughput Screening

Food Technology and Biotechnology, 2004
This paper reviews high-throughput screening enzyme assays developed in our laboratory over the last ten years. These enzyme assays were initially developed for the purpose of discovering catalytic antibodies by screening cell culture supernatants, but ...
Jean-Louis Reymond
doaj   +2 more sources

Whole organism high-content screening by label-free, image-based Bayesian classification for parasitic diseases. [PDF]

, 2012
Sole reliance on one drug, Praziquantel, for treatment and control of schistosomiasis raises concerns about development of widespread resistance, prompting renewed interest in the discovery of new anthelmintics.
Bickle, Quentin D, Bickle, Quentin D., Mansour Nuha R., Gilbert, Ian H, Alex E Benn, Bickle Quentin D., Ian H Gilbert, Nuha R. Mansour (146895), Andrew L. Hopkins (146939), Ivana Mikic (146920), Benn Alex E., Guidi Alessandra, Leo S. Bleicher (146907), Hopkins, Andrew L, Gilbert, Ian H., Bleicher Leo S., Hopkins Andrew L., Guidi, Alessandra, Benn, Alex E., Ivana Mikic, Quentin D. Bickle (146947), Alex E. Benn (146913), Irene Hallyburton (146900), Ian H. Gilbert (146934), Bleicher, Leo S, Alessandra Guidi (146927), Mansour, Nuha R., Paveley, Ross A., Alessandra Guidi, Bleicher, Leo S., Ross A Paveley, Irene Hallyburton, Quentin D Bickle, Hallyburton, Irene, Paveley Ross A., Paveley, Ross A, Mikic Ivana, Nuha R Mansour, Ross A. Paveley (146889), Mansour, Nuha R, Leo S Bleicher, Mikic, Ivana, Hopkins, Andrew L., Andrew L Hopkins, Hallyburton Irene, Gilbert Ian H., Benn, Alex E   +46 more
core   +3 more sources

siMacro: A Fast and Easy Data Processing Tool for Cell-Based Genomewide siRNA Screens [PDF]

Genomics & Informatics, 2013
Growing numbers of studies employ cell line-based systematic short interfering RNA (siRNA) screens to study gene functions and to identify drug targets.
Nitin Kumar Singh, Bo Yeun Seo, Mathukumalli Vidyasagar, Michael A. White, Hyun Seok Kim   +4 more
doaj   +1 more source

Assay Development for High-Throughput Drug Screening Against Mycobacteria [PDF]

Journal of Visualized Experiments
Mycobacterium abscessus (Mab) infections are challenging to treat due to high intrinsic drug resistance, comparable to multidrug-resistant tuberculosis. Treatments are extremely ineffective and based on a multi-drug regimen, resulting in low patient compliance. Consequently, the scientific community is urged to identify new and effective drugs to treat
Oliveira, Gabriel S., Bento, Clara M., Pombinho, António, Reis, Rita, Van Calster, Kevin, De Vooght, Linda, Maia, André F., Cos, Paul, Gomes, Maria Salomé, Silva, Tânia   +9 more
openaire   +5 more sources

FLICK: An optimized plate reader-based assay to infer cell death kinetics

STAR Protocols, 2021
Summary: Evaluating drug sensitivity is improved by directly quantifying death kinetics, rather than correlates of viability, such as metabolic activity.
Ryan Richards, Megan E. Honeywell, Michael J. Lee   +2 more
doaj   +1 more source

A high-throughput SARS-CoV-2 pseudovirus multiplex neutralization assay

STAR Protocols, 2022
Summary: Evaluating the neutralizing antibody titer following SARS-CoV-2 vaccination is essential in defining correlates of protection. We describe an assay that uses single-cycle vesicular stomatitis virus (VSV) pseudoviruses linking a fluorophore with ...
Benjamin Louis Sievers, Terri Gelbart, Gene S. Tan   +2 more
doaj   +1 more source

Microfluidic Technologies for High-Throughput Screening Applications [PDF]

, 2003
In this thesis, I present a strategy for the design and development of microfluidic devices for high-throughput screening applications, such as mutant enzyme libraries expressed in prokaryotic hosts, where a few point mutations at the DNA level ...
Thorsen, Todd Addison
core   +1 more source

A high-throughput protocol for monitoring starvation-induced autophagy in real time in mouse embryonic fibroblasts

STAR Protocols, 2021
Summary: Autophagy measurement has been challenging due to the transient nature of autophagy vesicles, in which degradation of cargo occurs. Here, we present a protocol to monitor starvation-induced autophagy using a live high-throughput microscopy ...
Ada Nowosad, Arnaud Besson
doaj   +1 more source