Results 111 to 120 of about 1,948,356 (316)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

Potentials of Dry Rotary Swaging

open access: yes, 2020
Infeed rotary swaging is an appropriate cold massive forging technology for the manufacture of cylindrical shaped components made of iron or aluminum base alloys.
Riemer, Oltmann   +6 more
core   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

open access: yesFEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel   +8 more
wiley   +1 more source

MiR‐513a promotes human erythroid differentiation by modulating c‐Jun

open access: yesFEBS Open Bio, EarlyView.
During early human erythropoiesis, miR‐513a promoted erythroid differentiation in primary human CD34+ hematopoietic stem‐progenitor cells and human TF‐1 erythroleukemic cells by indirectly decreasing c‐Jun and phospho‐c‐Jun expression, which are associated with increased GATA1 expression.
MinJung Kim   +11 more
wiley   +1 more source

Dry Hill Draggers

open access: yes, 1982
Photograph of the Dry Hill Draggers performing on stage at the World's Fair in Tennessee. Band members include: Carl Scott-fiddle, Monroe Boyd-guitar, Cordell Pinkerton-guitar, Jimmy Boyd-banjo, and Doug Cecil ...
Dry Hill Draggers;
core  

Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum

open access: yesFEBS Open Bio, EarlyView.
Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik   +6 more
wiley   +1 more source

How dry is dry? - A critical analysis of surface conditions used in dry metal forming

open access: yes, 2017
In the present study, the surface conditions of workpieces used for dry-metal forming experiments were analyzed. Spe-cifically, the effectiveness of different cleaning approaches was evaluated using x-ray photoelectron spectroscopy, in-frared ...
Maier, H. J.
core  

Assessment of seasonal water discharge trends and socio-economic impacts in the Karnakathi Canal, Barishal

open access: yesWater Science
Stream discharge is a critical hydrological parameter influenced by the natural flow characteristics of the channel and is essential for effective water resource management.
Md. Masum Billah, Md. Helal Ahmmed
doaj   +1 more source

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