Results 71 to 80 of about 314,284 (266)

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Effects of Different Drying Methods on Nutritional Components, Antioxidant Activity and Volatile Components of Five Kinds of Dried Figs

Shipin gongye ke-ji
Drying methods may cause changes in the color and texture of figs, accelerating the loss of nutrients. In this study, five fig varieties ('Boji red' 'Orphan' 'Zhongzi' 'Qingpi' and 'Xinjiang Zaohuang') were used as research subjects.
Zhenzhen DING, Na XIA, Yanquan LIU, Tian ZHANG, Lei JIANG, Yanchang WANG, Chao ZHANG   +6 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Effects of different drying methods on drying characteristics and quality of taro strips

Shipin yu jixie
ObjectiveTo reduce postharvest spoilage of taro and improve the drying quality of taro strips.MethodsFour drying methods, i.e., vacuum microwave drying (VMD), hot air drying (HAD), heat pump drying (HPD), and far-infrared radiation drying (FIRD), were ...
LI Xiaoyun, XIE Ronghua, CHEN Sheng, HAN Chunyang, CHEN Junliang, REN Aiqing, DUAN Zhenhua   +6 more
doaj   +1 more source

MiR‐513a promotes human erythroid differentiation by modulating c‐Jun

FEBS Open Bio, EarlyView.
During early human erythropoiesis, miR‐513a promoted erythroid differentiation in primary human CD34+ hematopoietic stem‐progenitor cells and human TF‐1 erythroleukemic cells by indirectly decreasing c‐Jun and phospho‐c‐Jun expression, which are associated with increased GATA1 expression.
MinJung Kim, Brittany Taylor, Shannon Bolten, Christian L. Eberly, Mahliya Abdurahman, T. Michael Creed, Acong Yang, Taylor L. Hatchet, Tristan Dyson, Shuo Gu, Curt I. Civin, Tami J. Kingsbury   +11 more
wiley   +1 more source

Optimization of sulfur-free color protection process and comparison of drying techniques for honeysuckle

Shipin yu jixie
ObjectiveTo develop green processing technology to inhibit the browning of fresh honeysuckle (Lonicera japonica Thunb) and enhance its drying quality.MethodsWith polyphenol oxidase relative enzyme activity and color difference values as evaluation ...
ZHAI Yumei, TANG Xiaojuan, FU Xiao, GONG Min, LIU Yunguo   +4 more
doaj   +1 more source

Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum

FEBS Open Bio, EarlyView.
Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik, Russell Pickford, Hannah C. Timmins, Olivier Piguet, Glenda M. Halliday, Matthew C. Kiernan, Woojin Scott Kim   +6 more
wiley   +1 more source

Effects of five drying methods on the drying characteristics and quality of peanuts

Shipin yu jixie
ObjectiveTo determine the appropriate mechanical drying technology for peanuts.MethodsFresh peanuts were used as raw materials. The effects of heat pump drying (HPD), far infrared drying (FID), electric heating drying (EHD), far infrared combined heat ...
XIE Yongkang, WANG Xi, JU Haoyu, WANG Weina, WANG Xiaodong   +4 more
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source