Results 81 to 90 of about 357,614 (307)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Study on Heat Pump Low Temperature Drying of Momordica Fruit

Zhileng xuebao, 2014
In recent years, Momordica fruit, as a kind of high nutritious fruit, has been developing rapidly in Vietnam. However, the drying studies for Momordica fruit is lacking. In this paper, the heat pump low temperature drying of Momordica fruit were studied
NGUYEN DINH DUC, Li Baoguo, Xue Yameng
doaj  

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Temperature During Convective Drying of Thin Flat Wet Materials

Известия высших учебных заведений и энергетических объединенний СНГ: Энергетика
. The basic laws of the kinetics of drying thin flat materials during the period of drop in the drying speed are outlined. A method for calculating the average integral temperature of wet material on the basis of the relative temperature coefficient of ...
A. I. Ol’shanskii, A. A. Kotow, S. M. Kuzmenkov   +2 more
doaj   +1 more source

Anchorage‐independent and faster growth in clonal population from UV‐irradiated NER‐deficient cells

FEBS Open Bio, EarlyView.
UV‐irradiated cells expressing a DDB2 mutant protein unable to interact with PCNA (DDB2PCNA‐) form clones able to grow without anchorage. Different experimental approaches reveal heterogeneity in cell cycle regulation and drug response within these clones, emphasizing the crucial role of the DDB2‐PCNA interaction in preventing cellular transformation ...
Paola Perucca, Anna Tricarico, Martina Furfaro, Priyam Ghosh, Ennio Prosperi, Lucia Anna Stivala, Ornella Cazzalini   +6 more
wiley   +1 more source

Advance Drying Technology for Heat Sensitive Products [PDF]

, 2014
This book presents the advance drying technology for heat sensitive products cited from international journals, handbooks, and current research of authors. In the first edition, the printing and publication was funded Diponegoro University.
Djaeni, Mohamad, Suherman, Suherman, Sumardiono, Siswo   +2 more
core  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

A Comparison of Four Mower Conditioners on Drying Rate and Leaf Loss in Alfalfa and Agrass [PDF]

, 2000
Mechanical conditioning of forage can be accomplished by passing the crop through fluted intermeshing rolls or by passing the crop over the tines of an impeller rotor.
Bailey, Theodore, Greenlees, Wallace, Hanna, H. Mark, Hanna, H. Mark, Marley, Stephen, Shinners, Kevin   +5 more
core   +2 more sources

Study on a Moisture Ratio Curve Model for Refractance Window Drying Based on a D-Optimal Mixture Design

Journal of Food Quality
Refractance window (RW) drying is a new thin-layer drying technology that can control well the heating temperature to effectively reduce the loss of heat-sensitive substances.
Jingyu He, Weidong Song, Jianqiang Li, Tianhang Ding, Jian Guan, Jinji Wu, Jiaoling Wang   +6 more
doaj   +1 more source