Results 181 to 190 of about 1,079,354 (307)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Acute Clinical Effects of Dual-Task Resistance Exercise on Muscle Endurance and Pressure Sensitivity in Patients With Carpal Tunnel Syndrome After Surgery. [PDF]

Musculoskeletal Care
Núñez-Cortés R, Cruz-Montecinos C, López-Bueno L, López-Bueno R, Suso-Martí L, Salazar-Méndez J, Méndez-Rebolledo G, Morral A, Andersen LL, Calatayud J.   +9 more
europepmc   +1 more source

Dual-pump Kerr micro-cavity optical frequency comb with varying FSR spacing

, 2016
In this paper, we demonstrate a novel dual-pump approach to generate robust optical frequency comb with varying free spectral range (FSR) spacing in a CMOS-compatible high-Q micro-ring resonator (MRR). The frequency spacing of the comb can be tuned by an
Leiran Wang (3962789), Alessia Pasquazi (4464712), Feitao Li (16235537), Guoxi Wang (3957476), Wei Zhao (14321), Weiqiang Wang (400116), Yulong Su (3455321), Lei Wang (6656), Yuanshan Liu (11550514), Hui Hu (377207), Xiaohong Hu (308716), Yishan Wang (777325), Wenfu Zhang (2281144), Sai T Chu (4822308), Brent E Little (13960143)   +14 more
core  

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source

Tri-use of alcohol, cannabis, and nicotine in daily life among young adults: A day-level comparison of substance use outcomes to dual-use and alcohol-only use occasions. [PDF]

Exp Clin Psychopharmacol
Linden-Carmichael AN, Shipley JL, Sherman AL, Stamates AL.   +3 more
europepmc   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Correction: Viral escape-inspired framework for structure-guided dual bait protein biosensor design. [PDF]

PLoS Comput Biol
Teoh YC, Noor MS, Aghakhani S, Girton J, Hu G, Chowdhury R.   +5 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Is multitasking efficient? Different metrics, different conclusions. [PDF]

Psychol Res
Jung AC, Janczyk M, Manzey D, Fischer R.
europepmc   +1 more source

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

FEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui, Taobo Hu, Thomson Christian Husein, Wai Hei Shek, Bingrong Chen, Depeng Wang, Shu Wang, Weichuan Yu, Xiaodan Fan, Mengping Long, Toyotaka Ishibashi   +10 more
wiley   +1 more source