Results 21 to 30 of about 43,950 (246)
A Hybrid Model for Safety Pharmacology on an Automated Patch Clamp Platform: Using Dynamic Clamp to Join iPSC-Derived Cardiomyocytes and Simulations of Ik1 Ion Channels in Real-Time [PDF]
Frontiers in Physiology, 2018 An important aspect of the Comprehensive In Vitro Proarrhythmia Assay (CiPA) proposal is the use of human stem cell-derived cardiomyocytes and the confirmation of their predictive power in drug safety assays.
Birgit Goversen +7 more
doaj +2 more sources
High-speed dynamic-clamp interface.
J Neurophysiol, 2015 The dynamic-clamp technique is highly useful for mimicking synaptic or voltage-gated conductances. However, its use remains rare in part because there are few systems, and they can be expensive and difficult for less-experienced programmers to implement.
Yang Y +4 more
europepmc +6 more sources
Causes of Transient Instabilities in the Dynamic Clamp [PDF]
IEEE Transactions on Neural Systems and Rehabilitation Engineering, 2009 The dynamic clamp is a widely used method for integrating mathematical models with electrophysiological experiments. This method involves measuring the membrane voltage of a cell, using it to solve computational models of ion channel dynamics in real-time, and injecting the calculated current(s) back into the cell. Limitations of this technique include
Robert Butera
exaly +3 more sources
The human dynamic clamp as a paradigm for social interaction. [PDF]
Proc Natl Acad Sci U S A, 2014 Significance The human dynamic clamp (HDC) is proposed as a general paradigm for studies of elementary forms of social behavior in complex biological systems. HDC enables parametric control of real-time bidirectional interaction between humans and empirically grounded theoretical models of coordination dynamics.
Dumas G +3 more
europepmc +4 more sources
Dynamic clamp with StdpC software. [PDF]
Nat Protoc, 2011 Dynamic clamp is a powerful method that allows the introduction of artificial electrical components into target cells to simulate ionic conductances and synaptic inputs. This method is based on a fast cycle of measuring the membrane potential of a cell, calculating the current of a desired simulated component using an appropriate model and injecting ...
Kemenes I +6 more
europepmc +5 more sources
eLife, 2022 Reciprocal inhibition is a building block in many sensory and motor circuits. We studied the features that underly robustness in reciprocally inhibitory two neuron circuits.
Ekaterina Morozova +2 more
doaj +1 more source
Circulation: Arrhythmia and Electrophysiology, 2015 Mutations in KCNH2 (hERG) underlie the long-QT2 syndrome. Most long-QT2 mutations produce a trafficking defect resulting in a decrease in n, the number of functional channels on the cell surface. Some mutations can also alter gating characteristics.
Thomas Nowotny, Pablo Varona
openaire +3 more sources
eLife, 2020 Across biological systems, cooperativity between proteins enables fast actions, supra-linear responses, and long-lasting molecular switches. In the nervous system, however, the function of cooperative interactions between voltage-dependent ionic channels
Paul Pfeiffer +5 more
doaj +1 more source
eLife, 2020 Voltage-gated sodium channels play a critical role in cellular excitability, amplifying small membrane depolarizations into action potentials. Interactions with auxiliary subunits and other factors modify the intrinsic kinetic mechanism to result in new ...
Marco A Navarro +6 more
doaj +1 more source
Loading Dynamics of a Sliding DNA Clamp [PDF]
Angewandte Chemie, 2014 AbstractSliding DNA clamps are loaded at a ss/dsDNA junction by a clamp loader that depends on ATP binding for clamp opening. Sequential ATP hydrolysis results in closure of the clamp so that it completely encircles and diffuses on dsDNA. We followed events during loading of an E. coli β clamp in real time by using single‐molecule FRET (smFRET).
Cho, Won-Ki +4 more
openaire +5 more sources