Results 41 to 50 of about 9,178 (186)

Analytical validation of anti-toxoplasma IgG immunoassays

Brazilian Journal of Infectious Diseases, 2012
There are often discrepancies when using different methods to measure anti-Toxoplasma gondii IgG levels in patient samples. The diagnostic performance of a chemiluminescent immunoassay (CLIA) and an enzyme-linked fluorescent assay (ELFA) used as ...
Guenael Freire de Souza, Darlene Carvalho, William Pedrosa, Jacqueline Franck, Renaud Piarroux   +4 more
doaj   +5 more sources

Performance of a flow cytometry-based immunoassay for detection of antibodies binding to SARS-CoV-2 spike protein

Scientific Reports, 2022
The performance of a laboratory-developed IgG/IgA flow cytometry-based immunoassay (FCI) using Jurkat T cells stably expressing full-length native S protein was compared against Elecsys electrochemiluminiscent (ECLIA) Anti-SARS-CoV-2 S (Roche Diagnostics,
Arantxa Valdivia, Fabián Tarín, María Jesús Alcaraz, Paula Piñero, Ignacio Torres, Francisco Marco, Eliseo Albert, David Navarro   +7 more
doaj   +1 more source

Validation of a SARS-CoV-2 Surrogate Virus Neutralization Test in Recovered and Vaccinated Healthcare Workers

Viruses, 2023
Vaccination against COVID-19 is the main public health approach to fight against the pandemic. The Spike (S) glycoprotein of SARS-CoV-2 is the principal target of the neutralizing humoral response. We evaluated the analytical and clinical performances of
Lina Mouna, Mehdi Razazian, Sandra Duquesne, Anne-Marie Roque-Afonso, Christelle Vauloup-Fellous   +4 more
doaj   +1 more source

Clinical and molecular fingerprint of SARS-CoV-2 among hospital employees in a period of Omicron BA.2 dominance

GMS Hygiene and Infection Control
In the spring of 2022, SARS-CoV-2 Omicron BA.2 peaked in Germany. The main burden was staff shortage. To achieve effective identification and management of infected persons as well as early reintegration of recovered persons, an infection-control ...
Rauschning, Dominic, Weppler, Ruth, Balczun, Carsten, Scheumann, Gwendolyn, Monteiro Marques, Jasmin, Mutschnik, Christina, Preuß, Dominic, Schmithausen, Ricarda Maria, Starke, Maximilian, Hagen, Ralf Matthias, Döhla, Manuel   +10 more
doaj   +1 more source

Performance of LFA and ECLIA in detecting anti-SARS-CoV-2 antibodies.

, 2021
Anti-SARS-CoV-2 IgM (A) and IgG (B) antibodies were detected using a lateral flow immunochromatographic assay (LFA), and anti-SARS-CoV-2 total antibodies were detected using an electrochemiluminescence immunoassay (ECLIA).
Hiroo Hasegawa (3586247), Kouichi Morita (201947), Shigeru Kohno (278864), Yui Shigeishi (11495246), Kenji Ota (7320323), Kosuke Kosai (535643), Jiro Yasuda (32563), Hironori Sakai (449393), Koichi Izumikawa (535642), Fumitaka Nishimura (11495243), Koya Ariyoshi (7877), Norihito Kaku (7439288), Rina Tachiki (11495249), Kei Sakamoto (402255), Hiroshi Mukae (409973), Katsunori Yanagihara (535647), Daisuke Sasaki (82102)   +16 more
core   +1 more source

EDTA-Anticoagulated Whole Blood for SARS-CoV-2 Antibody Testing by Electrochemiluminescence Immunoassay (ECLIA) and Enzyme-Linked Immunosorbent Assay (ELISA)

, 2020
While lateral flow test formats can be utilized with whole blood and low sample volumes, their diagnostic characteristics are inferior to immunoassays based on chemiluminescence immunoassay (CLIA) or enzyme-linked immunosorbent assay (ELISA) technology ...
Thomas Bodmer, Mauro Imperiali, Philipp Kohler, Kirsten Grossmann, Lorenz Risch, Myriam Weber, Dorothea Hillmann, Susanna Bigler, Thomas Lung, Pietro Vernazza, Nadja Wohlwend, Yacir Salimi, Michael Ritzler, Martin Risch, Matthias Paprotny, Marc Kovac, Sonja Heer, Francesca Ferrara, Christian R. Kahlert, Sarah Thiel, Konrad Egli, Harald Renz, Kohler P, Lung T, Salimi Y, Kovac M, Bigler S, Vernazza P, Grossmann K, Thiel S, Imperiali M, Paprotny M, Ferrara F, Kahlert CR, Risch M, Weber M, Bodmer T, Risch L, Ritzler M, Heer S, Egli K, Renz H, Hillmann D, Wohlwend N   +43 more
core   +1 more source

Quantification of Tau-A in serum after brain injury: a comparison of two analytical platforms, ELISA and electrochemiluminescence immunoassay

, 2022
Background: Previous studies have indicated the utility of the ADAM10-generated fragment of tau, Tau-A, as marker of neuronal damage. However, the sensitivity of the ELISA-based system was limited.
Tzara, Ourania, Sode West, Anders, Asser Karsdal, Morten, Henriksen, Kim, Iversen, Helle Klingenberg, West, Anders Sode, Klingenberg Iversen, Helle, Amalie Simonsen, Sofie, Helle Klingenberg Iversen, Ourania Tzara, Anders Sode West, Sofie Amalie Simonsen, Morten Asser Karsdal, Kim Henriksen   +13 more
core   +1 more source

Seroprevalence of anti-SARS-CoV-2 antibodies among blood donors from December 2020 to June 2021 in Koutiala district, Mali

PLOS Global Public Health, 2023
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the virus associated with coronavirus disease (COVID-19). At the time of the study, little data on the level of exposure of the population in Koutiala district in Mali to SARS-CoV-2 was ...
Fara Wagbo Temessadouno, Jean Gilbert Ndong, Etienne Gignoux, Yves Coppieters, Alhassane Ba, Youssouf Diam Sidibe, Aminata Daou, Nada Malou, Idrissa Compaore, Tidiani Fane, Erica Simons, Francisco Luquero, Clair Mills, Komla Mawunya Vuti, Marie Hortense Nkokolo massamba, Sonia Guiramand   +15 more
doaj   +2 more sources

Rational Control of Antibody Orientation for Enhancing Sensitivity and Specificity in Lateral Flow Immunoassays With Clinical Validation

Advanced Healthcare Materials, EarlyView.
Systematic investigation of antibody orientation strategies in lateral flow immunoassays reveals that a para‐substituted (aminomethyl)phenylboronic acid mediated probe (P‐6) combined with a biotin‐streptavidin test‐line modification achieves a synergistic 14‐ to 19‐fold sensitivity gain over conventional conjugation. Furthermore, multiplexed detection,
Pin‐Hao Wang, Shunmuga Nathan Shunmuga Nainar, Bing‐Han Zhuang, Shun‐Mao Yang, Hong‐Cheu Lin, Yang‐Hsiang Chan   +5 more
wiley   +1 more source

Comparison of CBA, MSD ECLIA and SIMOA methods using lysate samples.

, 2018
The techniques were compared two by two, and slopes and y-intercepts (including 95% CI) were computed for GAD65 values 2 = 0.998. (B) CBA/SIMOA: slope (95%CI) = 0.94 (0.90–0.99); y-intercept (95%CI) = -1.81 (-6.51–2.88) ng/dL; R2 = 0.997.
Sarah Roels (4518385), Katrijn Verhaeghen (235860), Daniel Pipeleers (164901), Zhidong Ling (164900), Geert A. Martens (164886), Geert Stangé (206966), Olivier R. Costa (4922968), Frans K. Gorus (93159)   +7 more
core   +1 more source