Results 121 to 130 of about 1,522,114 (310)
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point +7 more
wiley +1 more source
IMPLEMENTASI ALGORITMA GREEDY UNTUK MELAKUKAN GRAPH COLORING: STUDI KASUS PETA PROPINSI JAWA TIMUR
This paper will describe us how to coloring a graph by using greedy algorithm with the case study province of Jawa Timur. From this research we will know that for graph coloring at Jawa Timur Province only use four difference colors.
Ardiansyah Ardiansyah +5 more
doaj +1 more source
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley +1 more source
Dapagliflozin prevents methylglyoxal‐induced retinal cell death in ARPE‐19 cells
Diabetic macular oedema is a diabetes complication of the eye, which may lead to permanent blindness. ARPE‐19 are human retinal cells used to study retinal diseases and potential therapeutics. Methylglyoxal is a compound increased in uncontrolled diabetes due to elevated blood glucose.
Naina Trivedi +7 more
wiley +1 more source
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn +4 more
wiley +1 more source
This paper has been withdrawn by the author due to an error in the proof.
openaire +2 more sources
Maximal edge colorings of graphs
For a graph $G$ of order $n$ a maximal edge coloring is a proper edge coloring with $ '(K_n)$ colors such that adding any edge to $G$ in any color makes it improper. Meszka and Tyniec proved that for some values of the number of edges there are no graphs with a maximal edge coloring, while for some other values, they provided constructions of such ...
Babiński, Sebastian, Grzesik, Andrzej
openaire +6 more sources
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Linear-time algorithm for the edge-colorability of a graph with prescribed vertex types [PDF]
We consider the coloring of edges in a graph in which there are vertices of three types. In a feasible edge coloring, each vertex of the first type is incident with at least two edges of the same color, and each vertex of the second type with at least ...
Zsolt Tuza, Vitaly Voloshin
doaj
Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth +6 more
wiley +1 more source

