Results 11 to 20 of about 9,543 (298)

A manager’s guide to using eDNA metabarcoding in marine ecosystems [PDF]

PeerJ, 2022
Environmental DNA (eDNA) metabarcoding is a powerful tool that can enhance marine ecosystem/biodiversity monitoring programs. Here we outline five important steps managers and researchers should consider when developing eDNA monitoring program: (1 ...
Zachary Gold, Adam R. Wall, Teia M. Schweizer, N. Dean Pentcheff, Emily E. Curd, Paul H. Barber, Rachel S. Meyer, Robert Wayne, Kevin Stolzenbach, Kat Prickett, Justin Luedy, Regina Wetzer   +11 more
doaj   +5 more sources

Trait-Based Biomonitoring Using eDNA Metabarcoding to Assess Anthropogenic Disturbances on Freshwater Fish Communities. [PDF]

Mol Ecol Resour
ABSTRACT Various anthropogenic disturbances affect the succession of aquatic habitats along dendritic river networks. Bioindicator taxa, such as fish, can be used to assess the effects of these disturbances on habitat quality. Environmental DNA (eDNA) metabarcoding offers a novel approach to complement traditional sampling and analysis of bioindicator ...
Morissette O, Côté G, Couillard MA, Pouliot R, Bernatchez L.   +4 more
europepmc   +3 more sources

Unveiling Vertebrate Biodiversity in Arid and Semi‐Arid Terrestrial Ecosystems Through eDNA Metabarcoding at Savanna Waterholes [PDF]

Evolutionary Applications
Applying environmental DNA (eDNA) metabarcoding to samples from waterholes and their surroundings offers a promising approach for monitoring terrestrial vertebrates in semi‐arid and arid ecosystems, such as the southern African savannas. However, minimal
Tamara Schenekar, Janine Baxter, Irmgard Sedlmayr, Julia Gladitsch, Sibusiso Mahlangu, Monica Mwale   +5 more
doaj   +2 more sources

Roaming the Seas—Assessing Marine Invertebrate Biodiversity Along Salinity Gradients With Zooplankton and eDNA Metabarcoding [PDF]

Environmental DNA
Marine metazoan biodiversity is accretively being explored through environmental DNA (eDNA) metabarcoding of seawater. However, knowledge gaps in the use of eDNA to study changes in diversity resulting from changing abiotic conditions still do exist.
Alica Ohnesorge, Uwe John, Lucie Kuczynski, Stefan Neuhaus, Kingsly Chuo Beng, Bernd Krock, Silke Laakmann   +6 more
doaj   +2 more sources

A performance evaluation of targeted eDNA and eDNA metabarcoding analyses for freshwater fishes

Environmental DNA, 2019
AbstractBackgroundThe use of environmental DNA analysis has revolutionized biodiversity monitoring. Initially, eDNA monitoring surveys in aquatic environments utilized a targeted approach, but there has been a steady shift toward whole community assessments (eDNA metabarcoding).
Jonas Bylemans, Dianne M. Gleeson, Richard P. Duncan, Christopher M. Hardy, Elise M. Furlan   +4 more
openaire   +3 more sources

How does eDNA decay affect metabarcoding experiments? [PDF]

Environmental DNA, 2022
The detection of species using environmental DNA (eDNA) relies on our capacity to identify DNA from the sampled environment. Once eDNA is released into the environment, the physical degradation of individual eDNA molecules over time directly affects our ...
Luke E. Holman, Yi Chng, Marc Rius
doaj   +3 more sources

eDNA metabarcoding outperforms traditional fisheries sampling and reveals fine‐scale heterogeneity in a temperate freshwater lake

Environmental DNA, 2021
Understanding biodiversity in aquatic systems is critical to ecological research and conservation efforts, but accurately measuring species richness using traditional methods can be challenging.
Rebecca R. Gehri, Wesley A. Larson, Kristen Gruenthal, Nicholas M. Sard, Yue Shi   +4 more
doaj   +2 more sources

Toward freshwater plant diversity surveys with eDNA barcoding and metabarcoding

Environmental DNA, 2023
Providing reliable, cost‐effective data on species distribution is critical to ensuring biodiversity conservation. However, many species may go unrecorded by conventional surveys, especially in aquatic environments. Environmental DNA (eDNA) barcoding and
Armando Espinosa Prieto, Jean‐Nicolas Beisel, Pieternel Verschuren, Laurent Hardion   +3 more
doaj   +3 more sources

Benchmarking bioinformatic tools for fast and accurate eDNA metabarcoding species identification

Molecular Ecology Resources, 2021
AbstractBioinformatic analysis of eDNA metabarcoding data is a crucial step toward rigorously assessing biodiversity. Many programs are now available for each step of the required analyses, but their relative abilities at providing fast and accurate species lists have seldom been evaluated.
Mathon, Laëtitia, Valentini, Alice, Guerin, Pierre-Edouard, Normandeau, Eric, Noël, Cyril, Lionnet, Clément, Boulanger, Emilie, Thuiller, Wilfried, Bernatchez, Louis, Mouillot, David, Dejean, Tony, Manel, Stéphanie   +11 more
openaire   +7 more sources

Application of eDNA metabarcoding in a fragmented lowland river: Spatial and methodological comparison of fish species composition

Environmental DNA, 2021
Assessments of fish communities tend to rely on capture‐based methods that, due to sampling biases, can underestimate actual species richness. Alternatively, environmental DNA (eDNA) based metabarcoding is a noncapture approach that infers species ...
Caterina M. Antognazza, Robert J. Britton, Daniel S. Read, Tim Goodall, Anna Mantzouratou, Vanessa De Santis, Peter Davies, Miran Aprahamian, Elizabeth Franklin, Emilie A. Hardouin, Demetra Andreou   +10 more
doaj   +2 more sources