Results 91 to 100 of about 1,639,498 (342)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

The Lyman-alpha forest at redshifts 0.1 -- 1.6: good agreement between a large hydrodynamic simulation and HST spectra

, 2008
We give a comprehensive statistical description of the Lyman-alpha absorption from the intergalactic medium in a hydrodynamic simulation at redshifts 0.1-1.6, the range of redshifts covered by HST spectra of QSOs.
Abel, Anninos, Bagla, Barkana, Bechtold, Bodenheimer, Bolton, Bolton, Bolton, Bryan, Bryan, Bryan, Cen, Cen, Cen, Croft, Dave, David Kirkman, David Tytler, Dav��, Dav��, Dunkley, Eisenstein, Fang, Gazta��aga, Haardt, Haardt, Heap, Hernquist, Hui, Janknecht, Jena, Kim, Kim, Kirkman, Kirkman, Kirkman, Kirkman, Kohler, Ma, Meiksin, Michael L. Norman, Milutinovi��, Miralda-Escude, Misawa, Norman, Norman, O'Shea, O'Shea, Pascal Paschos, Pen, Rauch, Regan, Riediger, Spergel, Spitzer, Theuns, Tridivesh Jena, Tytler, Tytler, Tytler, Weymann, Zhang   +62 more
core   +1 more source

Extension of nano-confined DNA: quantitative comparison between experiment and theory [PDF]

, 2015
The extension of DNA confined to nanochannels has been studied intensively and in detail. Yet quantitative comparisons between experiments and model calculations are difficult because most theoretical predictions involve undetermined prefactors, and ...
Ambjörnsson, T., Beech, J. P., Fritzsche, J., Iarko, V., Mehlig, B., Mehlig, K., Müller, V., Nyberg, L. K., Tegenfeldt, J. O., Werner, E., Westerlund, F.   +10 more
core   +2 more sources

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Effect of the Effective Clear Width of Door in a Modular Classroom on Evacuation Time [PDF]

, 2023
Seon Yong Jeong, Hong Sang Lee, Ha Sung Kong   +2 more
openalex   +1 more source

Identifying gene expression signatures for risk stratification of postoperative adjuvant chemotherapy in colorectal cancer

FEBS Open Bio, EarlyView.
A novel signature integrating genome‐wide analysis with clinical factors predicts recurrence in stage II colorectal cancer and enables a new risk stratification to guide postoperative adjuvant chemotherapy. Clinical risk stratification for postoperative recurrence in patients with pathological stage II (pStage II) colorectal cancer (CRC) is essential ...
Mayuko Otomo, Keisuke Okuno, Shuichi Watanabe, Sakiko Oba, Hiroyasu Kagawa, Masanori Tokunaga, Daisuke Ban, Yusuke Kinugasa   +7 more
wiley   +1 more source

Cutaneous Melanoma Drives Metabolic Changes in the Aged Bone Marrow Immune Microenvironment

Aging and Cancer, EarlyView.
Melanoma, the deadliest form of skin cancer, increasingly affects older adults. Our study reveals that melanoma induces changes in iron and lipid levels in the bone marrow, impacting immune cell populations and increasing susceptibility to ferroptosis.
Alexis E. Carey, Murilo Ramos Rocha, Cheyenne Palm, Vania Wang, Denys Balandin, Ethan Black, Kevin Y. Zhang, Fan Huang, Daniel J. Zabransky, Yash Chhabra, Mareike Peters, Gabriel Ghiaur, Ashani Weeraratna   +12 more
wiley   +1 more source

Experimental Research on Bending Performance of Wood-concrete Composite Slab with Screw Connections

BioResources
The existing research primarily focuses on wood-concrete composite beams, with limited studies on the bending performance and effective width of wood-concrete composite slabs.
Hao Du, Jiangtao Mei, Weijie Fu, Xiamin Hu   +3 more
doaj   +2 more sources

HUBUNGAN PANJANG ANTRIAN KENDARAAN TERHADAP BERHENTINYA ANGKUTAN UMUM

Civil Engineering Dimension, 1999
Queue length of vehicles due to stopping of a public vehicle, cause traffic jam. To relate the influence of vehicle volume, by effective width of the road and public vehicle’s stopping time to queue length, linear regression model is used.
Bastian Wirantono, Prieska Prieska, Fanny Judodihardjo   +2 more
doaj