Results 221 to 230 of about 14,114 (244)
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Simple bioseparations using self-cleaving elastin-like polypeptide tags
Nature Methods, 2005We introduce a new method for the purification of recombinant proteins expressed in Escherichia coli using self-cleaving elastin-like polypeptide (ELP) fusion tags without the need for affinity chromatography or proteolytic tag removal. Using this method we obtained high purity, activity and reasonable yields for ten diverse target proteins.
Mahmoud Reza, Banki +2 more
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Cononsolvency of Elastin-like Polypeptides in Water/Alcohol Solutions
Biomacromolecules, 2019Elastin-like polypeptides (ELPs) are one of the most widely-studied classes of protein material because of their lower critical solution temperature (LCST)-like thermoresponsive behavior in aqueous solutions. Here, it is shown that ELPs also exhibit cononsolvency effects, similar to many other water-soluble polymers.
Carolyn E. Mills +2 more
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Cellular response to nanoscale elastin-like polypeptide polyelectrolyte multilayers
Acta Biomaterialia, 2008Ionic elastin-like polypeptide (ELP) conjugates are a new class of biocompatible, self-assembling biomaterials. ELPs composed of the repeat unit (GVGVP)(n) are derived from the primary sequence of mammalian elastin and produced in Escherichia coli.
M, Swierczewska +5 more
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Elastin-Like Polypeptides as Thermosensitive Polymer System
Advanced Materials Research, 2014Thermo-responsive elastin-like polypeptides (ELPs) were successfully obtained by inverse transition cycling (ITC) and recursive directional ligation (RDL). Six ELPs displayed thermal properties, depending on their sequence and chain length. It was found that the ELP[KV8F-4 and ELP[KV8F-8 were effective as thermosensitive materials at the body ...
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Elastin-like polypeptide-doxorubicin conjugates for cancer therapy
Proceedings of the Second Joint 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society] [Engineering in Medicine and Biology, 2003Thermally responsive elastin-like polypeptides (ELP) were synthesized by recombinant DNA techniques and conjugated to doxorubicin through an acid labile bond. The thermal properties, release of doxorubicin, uptake of ELP, cytotoxicity and cellular localization of ELP-doxorubicin conjugates were investigated in this study.
M. Dreher +5 more
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Steric stabilization of bioactive nanoparticles using elastin-like polypeptides
Advanced Drug Delivery ReviewsElastin-like polypeptides (ELP) are versatile, thermo-responsive polymers that can be conjugated to virtually any therapeutic cargo. Derived from short amino-acid sequences and abundant in humans, certain ELPs display low immunogenicity. Substrates for endogenous proteases, ELPs are biodegradable and thus, are candidate biomaterials.
Alvin, Phan, J Andrew, MacKay
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Thermosensitive Vesicles from Chemically Encoded Lipid‐Grafted Elastin‐like Polypeptides
Angewandte Chemie - International Edition, 2021Hang Zhao +2 more
exaly
Leukocyte Response to Elastin-Like Polypeptide Coatings
2013Small diameter synthetic vascular grafts have yet to be clinically successful due to luminal narrowing from thrombosis and intimal hyperplasia. Current attempts to address this issue include the development of materials that support endothelialisation and protein modification to the material surfaces that reduce thrombosis.
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Light‐Responsive Elastin‐Like Peptide‐Based Targeted Nanoparticles for Enhanced Spheroid Penetration
Angewandte Chemie - International Edition, 2023Jan van Hest
exaly