Results 101 to 110 of about 1,193,818 (298)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Proliferation and apoptosis features of ovarian follicles after local irradiation with electrons and platelet-rich plasma administration

RUDN Journal of Medicine
Relevance. The ovary is strongly radiosensitive organ. Exposure to ionizing radiation can lead to decreased reproductive function, including infertility.
Grigory Demyashkin, Zaira Murtazalieva, Ekaterina Pugacheva, Matvey Vadyukhin, Makka Bimurzaeva, Aigul Milovanova, Tamara Dengina   +6 more
doaj   +1 more source

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

FEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth, Moritz Koch, Le‐Han Rössner, Johanna Eichhorn, Denys Pogoryelov, Matthias P. Mayer, Jude M. Przyborski   +6 more
wiley   +1 more source

Electron Temperatures in the Venusian Ionosphere From Parker Solar Probe Using Quasi‐Thermal Noise Spectroscopy

Geophysical Research Letters
Parker Solar Probe (PSP) uses Venus gravity assists (VGA) to achieve the closest orbits to the Sun by a spacecraft. During the third (VGA3) and fourth (VGA4) Venus gravity assists, the PSP entered the Venusian ionosphere.
Speero M. Tannous, John W. Bonnell, Marc Pulupa, Stuart D. Bale   +3 more
doaj   +1 more source

Modeling the conditions for obtaining nanostructures during ion-plasma processing taking into account the quantum-mechanical properties of electrode material

Авіаційно-космічна техніка та технологія
The subject matter of the article is the thermophysical and mechanical properties of surface layers of structural materials using a quantum-mechanical approach.
Yurii Shyrokyi, Iurii Sysoiev, Yevhen Fesenko   +2 more
doaj   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Structural organization of p62 filaments and the cellular ultrastructure of calcium-rich p62-enwrapped lipid droplet cargo

Nature Communications
The selective autophagy receptor p62/SQSTM1 is known to form higher-order filaments in vitro and to undergo liquid-liquid phase separation when mixed with poly-ubiquitin.
Sabrina Berkamp, Lisa Jungbluth, Alexandros Katranidis, Siavash Mostafavi, Olivera Korculanin, Peng-Han Lu, Lotte Ickert, Maya M. Dierig, Lokesh Sharma, Lipi Thukral, Pitter F. Huesgen, Natalia L. Kononenko, Jörg Fitter, Rafal E. Dunin-Borkowski, Carsten Sachse   +14 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

FEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source