Results 101 to 110 of about 228,742 (299)

Directed Evolution Improves the Catalytic Efficiency of APEX2‐Mediated Proximity‐Dependent RNA Labeling

Advanced Science, EarlyView.
ABSTRACT Engineered ascorbate peroxidase APEX2 has been widely used for spatially restricted profiling of subcellular biomolecules, but its catalytic efficiency toward newly developed probes such as biotin‐aniline (Btn‐An) remains suboptimal. To overcome this limitation, we performed yeast surface display‐based directed evolution to enhance APEX2 ...
Gang Wang, Yi Li, Peiyuan Meng, Peng Zou
wiley   +1 more source

Essential lipid autacoids rewire mitochondrial energy efficiency in metabolic dysfunction‐associated fatty liver disease

Hepatology, EarlyView., 2022
Increased liver content of DHA‐derived small lipid autacoids (i.e resolvin D1 and maresin 1) associates with enhanced mitochondrial oxidative phosphorylation, fatty acid β‐oxidation and bioenergetic metabolic flux. These features provide hepatic protection from steatotic, pro‐inflammatory and fibrogenic insults.
Cristina López‐Vicario, David Sebastián, Mireia Casulleras, Marta Duran‐Güell, Roger Flores‐Costa, Ferran Aguilar, Juan José Lozano, Ingrid W. Zhang, Esther Titos, Jing X. Kang, Antonio Zorzano, Makoto Arita, Joan Clària   +12 more
wiley   +1 more source

Fluorogenic Coupled Assays Reveal Catalytic Properties, Inhibition Constants and Cellular Location of Mucin‐Active Carbohydrate Sulfatases

Angewandte Chemie, EarlyView.
We report plate‐based coupled assays to rapidly and quantitatively determine the activity and selectivity of mucin‐active carbohydrate sulfatases. This fluorogenic assay further enables inhibitor identification and allows determination of (sub‐)cellular location. ABSTRACT Sulfated glycans play a central role in human health and influence cell signaling,
Charles W. E. Tomlinson, Madouc D. Bergers, David N. Bolam, Ana S. Luis, Alan Cartmell, Zachary Armstrong   +5 more
wiley   +2 more sources

New analytical challenges in characterization of antibody-drug conjugates

Journal of Chromatography Open
Since 1913, when Paul Ehrlich introduced the concept of “selective toxicity”, new strategies to obtain quality control, quantification of the drug were mandatory. For this reason, the introduction of antibody drug conjugates (ADC) has shown many critical
Erika Maria Ricci, Enrico Dainese, Vincenzo De Laurenzi, Giulio Lovato, Gianluca Sala, Marcello Locatelli, Miryam Perrucci   +6 more
doaj   +1 more source

Statistical modeling of STR capillary electrophoresis signal

BMC Bioinformatics, 2019
Background In order to isolate an individual’s genotype from a sample of biological material, most laboratories use PCR and Capillary Electrophoresis (CE) to construct a genetic profile based on polymorphic loci known as Short Tandem Repeats (STRs).
Slim Karkar, Lauren E. Alfonse, Catherine M. Grgicak, Desmond S. Lun   +3 more
doaj   +1 more source

α_S1-casein in goat milk: identification of genetic variants by Capillary Zone Electrophoresis compared to Isoelectric Focusing [PDF]

, 2003
AlphaS1 casein fraction in caprine milk is characterized by an important polymorphism due to substitution, deletion of amino acids and post trascriptional modifications (Grosclaude et al., 1994; Ferranti et al., 1997).
Brambilla, A., Enne, Giuseppe, Feligini, Maria   +2 more
core  

Enhanced Intracellular Stability and Translation Efficiency of mRNA Drugs by a 2‐arm mRNA Platform

Advanced Science, EarlyView.
We constructed a 2‐arm mRNA, characterized by a unique topology formed through the dimerization of two mRNA 3’ tails. The 2‐arm mRNA improves 3’ tail stability and resistance to nuclease degradation, resulting in an intracellular half‐life of up to 65 h. This method substantially enhances the translation capacity of mRNA drugs.
Xucong Teng, Jiahao Lin, Qiushuang Zhang, Xiangdong Zhang, Yicong Dai, Jinghong Li   +5 more
wiley   +1 more source

Local Chemical Gradients in a Mammalian Cortex Measured In Vivo With a Silicon Nanodialysis Mass Spectrometry Platform

Angewandte Chemie, EarlyView.
Fluorescence image of the coronal slice of mouse brain where multiple neurochemicals have been measured in vivo with a nanodialysis platform coupled to CE‐MS to reveal strong millimeter‐scale spatial chemical gradients. Formation and maintenance of such local chemical compartments in the otherwise diffusion‐homogeneous cortex indicates strong ...
Weihua Shi, Keyin Li, Yu Ding, Alex G. Armstrong, Jonathan V. Sweedler, Yurii Vlasov   +5 more
wiley   +2 more sources

Possibilities of use of method of capillary electorphoresis while analyzing aqueous extract from saline soils

Бюллетень Почвенного института им. В.В. Докучаева, 2016
A comparative analysis of anions (Cl-, SO4 2-) and cations (Ca2+, Mg2+, Na+) detection in samples of saline soils by classical methods of titration (with use of atomic absorption analyzer Kvant-2AT for Na+ detection) and by method of capillary ...
G. I. Chernousenko, T. I. Borisochkina, N. S. Nikitina   +2 more
doaj   +1 more source

Field Amplified Separation in Capillary Electrophoresis:  A Capillary Electrophoresis Mode

Analytical Chemistry, 2006
In field-amplified injection in capillary electrophoresis (CE), the capillary is filled with two buffering zones of different ionic strength; this induces an amplified electrical field in the low ionic strength zone and a lower field in the high ionic strength zone, making sample stacking feasible.
Erny, Guillaume L., Cifuentes, Alejandro
openaire   +3 more sources