Results 41 to 50 of about 429,848 (299)

Plasma L-ergothioneine measurement by high-performance liquid chromatography and capillary electrophoresis after a pre-column derivatization with 5-iodoacetamidofluorescein (5-IAF) and fluorescence detection [PDF]

, 2013
Two sensitive and reproducible capillary electrophoresis and high-performance liquid chromatography-fluorescence procedures were established for quantitative determination of L-egothioneine in plasma.
Pintus, Gianfranco, Angelo Zinellu, Erre, Gian Luca, Carru, Ciriaco, Salvatore Sotgia, Ciriaco Carru, Sotgia, Salvatore, Erre GL, Gianfranco Pintus, Zinellu, Angelo, Deiana, Luca, Gerard Aime Pinna, Luca Deiana, Pisanu, Elisabetta, Elisabetta Pisanu, Gian Luca Erre, Pinna, Gérard Aimé, Sotgia S   +17 more
core   +1 more source

FUNDAMENTOS DE ELETROFORESE CAPILAR: UMA ABORDAGEM POR ANIMAÇÕES

Química Nova, 2015
Among the analytical separation techniques, capillary electrophoresis (CE) has attracted attention in both academic and industrial fields, mainly owing to its efficiency, short analysis times, low reagent consumption and residue generation.
Fernando Antonio Simas Vaz, Cledson Lopes Marta de Oliveira, Marcone Augusto Leal de Oliveira   +2 more
doaj   +1 more source

Enantiomer Separations by Capillary Electrophoresis

, 2016
Click on the DOI link to access the article May not be free).Capillary electrophoresis (CE) is a versatile and flexible technique for analytical enantioseparations.
Henrik Harnisch, Scriba, Gerhard K. E., Qingfu Zhu, Harnisch, Henrik, Zhu, Qingfu, Gerhard K. E. Scriba   +5 more
core   +1 more source

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Capillary electrophoresis-integrated immobilized enzyme reactors

Reviews in Analytical Chemistry, 2016
Online enzyme assay based on capillary electrophoresis (CE) offers several advantages for the assay, such as low consumption of samples, easy automation of all steps, and less requirement of sample work-up.
Liu Xiaoxia, Yang Jiqing, Yang Li
doaj   +1 more source

Structures of mycobacterial 3‐methylcrotonyl‐CoA carboxylase reveal carrier‐domain translocation between catalytic sites

FEBS Letters, EarlyView.
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav, Bogdan I. Florea, Sebastian Geibel   +2 more
wiley   +1 more source

20 Years of Fatty Acid Analysis by Capillary Electrophoresis

Molecules, 2014
A review taking into account the literature reports covering 20 years of fatty acid analysis by capillary electrophoresis is presented. This paper describes the evolution of fatty acid analysis using different CE modes such as capillary zone ...
Marcone Augusto Leal de Oliveira, Brenda Lee Simas Porto, Isaura Daniele Leite Faria, Patricia Lopes de Oliveira, Patricia Mendonça de Castro Barra, Renata de Jesus Coelho Castro, Renata Takabayashi Sato   +6 more
doaj   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

Data for semi-permanent cationic coating for protein separations

Data in Brief, 2020
Protein separations and analyses are fundamental to fields of study that include biochemistry, biology, physiology, drug discovery, pharmaceuticals, as well as agricultural and food based industries.
C.L. Crihfield, C.J. Kristoff, L.M. Veltri, C.A. Wilson, W.M. Penny, L.A. Holland   +5 more
doaj   +1 more source

Bi‐ and Mono‐Allelic RFC1 Expansion in a North American Cohort With Idiopathic Axonal Neuropathy

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective RFC1 biallelic repeat expansion is increasingly recognized as a cause of chronic idiopathic axonal polyneuropathy (CIAP), but it remains challenging to know who to test. This study aims to determine the prevalence of biallelic and monoallelic RFC1 expansions and their corresponding neuropathy phenotypes in CIAP patients and identify ...
Amro M. Stino, Lavanya Muthukumar, Evan L. Reynolds, Peter Todd, Sinem Ovunc, Sheng Chih Jin, Zitian Tang, Simone Thomas, PNRR Study Group, Ahmet Höke, Brian C. Callaghan, Sarah Berth, Vinay Chaudhry, David Cornblath, Leana Doherty, Lindsey Hayes, Hristelina Ilieva, Thomas Lloyd, Mohammad Khoshnoodi, Brett McCray, Brett Morrison, Bipasha Mukherjee‐Clavin, Lyle Ostrow, Michael Polydefkis, Ricardo Roda, Charlotte Sumner   +25 more
wiley   +1 more source