Results 51 to 60 of about 27,120 (238)
ABSTRACT The protein sample preparation methods for shotgun proteomics are nowadays well‐established unlike the ones for whole protein analysis. The goal of my work has been to create a simple methodology which provides a single uncomplicated sample preparation tool for these two fields. Nowadays the bulk of proteomics work is done using detergents for
Alexandre Zougman
wiley +1 more source
Comparison of effects of age and sex on serum protein electrophoretic pattern in one-humped camels (Camelus dromedarius) in Semnan, Iran [PDF]
The aim of this study was to evaluate the influence of age and sex on the concentration of total serum protein measured by the biuret method and protein fractions determined using cellulose acetate electrophoresis in apparently healthy camels (Camelus ...
M. Ahmadi-hamedani +4 more
doaj
18 days of predator odor exposure altered gut microbiota, increased anxiety‐like behaviors, and elevated hypothalamic–pituitary–adrenal axis activity in Brandt's voles, with males showing behavioral habituation and females remaining sensitive. Cecal microbiota transplantation experiments confirmed that only female recipients of microbiota from predator‐
Chen Gu +6 more
wiley +1 more source
Based on metagenome‐assembled genomes (MAGs), the gut microbiota of the gray snub‐nosed monkeys recovered 1229 non‐redundant MAGs. The gut microbiota showed an enhanced capacity to produce energy substrates with increased conversion activity of these substrates during winter.
Yue Sun +17 more
wiley +1 more source
Sulfated polysaccharides (SPs) have attracted growing interest for various biotechnological applications. We evaluated the efficiency of two methods of drying SPs (M I and II) extracted from Halymenia sp Rhodophyceae in order to compare the yield ...
José Ariévilo Gurgel Rodrigues +5 more
doaj +3 more sources
A simplified hemolysate-preparation reagent was composed of 1 g of ethylenediamine tetraacetic acid and 0.1 g of saponin dissolved in 500 ml of distilled water.
C Nopparatana +3 more
doaj
Survivability of lambs in relation to their dam's haemoglobin variants [PDF]
A total of 65 Yankasa, 23 Uda and 16 Balami ewes were mated to 4 Yankasa, 3 Uda and 3 Balami rams in a diallel breeding pattern to produce 192 lambs within 9 genotypes, which were used to study survivability of lambs in relation to their dam's ...
Iyiola-Tunji A.O. +3 more
doaj
Polyesterase activity and thermostability of carboxylesterases from Thermoleophilum album YS‐3
Three novel α/β‐hydrolases from thermophilic bacterium Thermoleophilum album display carboxylesterase and polyesterase activity. These enzymes hydrolyse PET, PLA and PCL both at high and moderate temperatures. TA21 shows superior activity, efficiently converting MHET to terephthalic acid. Structural features underlying substrate binding highlight their
Tatyana N. Chernikova +6 more
wiley +1 more source
Preliminary studies on ribonucleases from Poa pratensis seeds
Ribonuclease was extracted from Poa pratensis seeds with 0.1 M acetate buffer, pH 5.1, and then precipitated with alcohol. The enzyme was separated into 5 fractions (I-V) after chromatography on DEAE-cellulose at pH 5.1.
Irena Lorenc-Kubis +1 more
doaj +1 more source
A modified method used for the quantitative estimation of LDH-isoenzymes in serum after electrophoresis on cellulose acetate is described. Total LDH-activity and isoenzyme distribution in serum samples of capillary blood are compared to those in ...
Tabak-van Gorcum, J.A. +7 more
core +1 more source

