Results 141 to 150 of about 43,524 (303)

rDNAmine: A New Tool for the Analysis of Long Repetitive Sequences

Yeast, EarlyView.
This work introduces a novel approach for analysing long repetitive genomic sequences, combining a method to isolate large DNA molecules from individual chromosomes. We also describe the bioinformatics tool rDNAmine, which enables the isolation of long‐repeat sequences from reads obtained using the Oxford Nanopore method, as well as their preliminary ...
Agnieszka Czarnocka‐Cieciura, Natalia Gumińska   +1 more
wiley   +1 more source

Use of sterile gowns for single‐shot spinal anaesthesia: consensus guidelines from the Association of Anaesthetists, Royal College of Anaesthetists, Obstetric Anaesthetists' Association, Regional Anaesthesia UK, College of Anaesthesiologists of Ireland and Australian and New Zealand College of Anaesthetists

Anaesthesia, EarlyView.
Summary Introduction International guideline recommendations vary on the use of sterile gowns during spinal anaesthesia. There is limited evidence of benefit for their routine use and debate about environmental, financial and clinical costs and benefits.
Caroline Phillips, Manpreet Bahra, Yavor Metodiev, James H. Bamber, Dónall Ó Cróinín, Li Fang, Lucy Brooks, Helgi Jóhannsson, Nat Haslam, Archana Shrivathsa, Paul Southall, Cliff Shelton, Delphi collaborators, Alan J. R. Macfarlane, Archana Shrivathsa, Athmaja Thottungal, Caroline Phillips, Charlotte Oliver, Claire Abeysekera, Cliff Shelton, David Hewson, Dominique Nuala Lucas, Dónall Ó Cróinín, Emma Wain, Felicity Plaat, Gretel Davidson, Helgi Jóhannsson, Ian Blenkharn, Jaime Greenwood, James H. Bamber, Jane Pilsbury, Jenny Ferry, Joanna Marriott, Li Fang, Lloyd Turbitt, Lucy Brooks, Manpreet Bahra, Nat Haslam, Owen O'Sullivan, Philip Barclay, Rachel Mathers, Rebecca Knagg, Rosemary M. G. Hogg, Stephen Smith, Yavor Metodiev, Yasmin Poonawala   +45 more
wiley   +1 more source

Analysis of yeast DNA using pulsed field gel electrophoresis

, 2011
Technique of pulsed field gel electrophoresis (PFGE) has found widespread use in the analysis of the genome of all life organisms. It is applied to the separation of the large DNA molecules above thousands base pairs up to millions of base pairs in size,
Kubáčková, Martina
core  

A novel selective stabilizer of the ryanodine receptor 2 prevents stress‐induced ventricular arrhythmias without impairing cardiac function

British Journal of Pharmacology, EarlyView.
Abstract Background and Purpose Aberrant activation of type 2 ryanodine receptors (RyR2) causes lethal arrhythmias, such as catecholaminergic polymorphic ventricular tachycardia (CPVT). Developing drugs that suppress RyR2 hyperactivation may be key to novel arrhythmia treatments.
Nagomi Kurebayashi, Masami Kodama, Hana Inoue, Masato Konishi, Masami Sugihara, Takashi Murayama, Ryosuke Ishida, Koichiro Ishii, Shuichi Mori, Yukari Endo, Xi Zeng, Yukiko U. Inoue, Takayoshi Inoue, Satoru Noguchi, Hajime Nishio, Eri Nakashima, Norio Suzuki, Noriko Nakaya, Ryushi Sato, Yuya Otori, Ayuna Tsutsumi, Mirei Takahashi, Takayuki Oka, Haruo Ogawa, Kayoko Kanamitsu, Hiroyuki Kusuhara, Utako Yokoyama, Junko Kurokawa, Hiroyuki Kagechika, Takashi Sakurai   +29 more
wiley   +1 more source

Genotyping of methicillin-resistant Staphylococcus aureus isolates obtained in the Northeast region of Brazil

Brazilian Journal of Medical and Biological Research, 2009
Methicillin-resistant Staphylococcus aureus (MRSA) is a major agent of hospital infections worldwide. In Brazil, a multiresistant MRSA lineage (ST239-SCCmecIIIA), the so-called Brazilian epidemic clone (BEC), has predominated in all regions.
F.C. de Sousa-Junior, M.C. Silva-Carvalho, M.J.B.C. Fernandes, M.F.P. Vieira, F.L.P.C. Pellegrino, A.M.S. Figueiredo, M.C.N. de Melo, E.P. Milan   +7 more
doaj  

J Food Prot [PDF]

We report on a case of listeriosis in a patient who probably consumed a prepackaged romaine lettuce-containing product recalled for Listeria monocytogenes contamination.

core  

Toll‐like receptor 4 mediates lipopolysaccharide‐induced emesis

British Journal of Pharmacology, EarlyView.
Background and Purpose Lipopolysaccharide (LPS) induces inflammation and sepsis through Toll‐like receptor 4 (TLR4) activation. Common laboratory animals do not exhibit emesis, but administration of LPS in piglets, cats, and dogs induces emesis via peripheral mechanisms.
Luping Liu, Zengbing Lu, Julia Yuen Hang Liu, Man Piu Ngan, Kai‐Hei Tse, John Anthony Rudd   +5 more
wiley   +1 more source

Molecular characterization of Escherichia coli isolates obtained from beef using rapid pulsed-field gel electrophoresis [PDF]

, 2003
Rapid pulsed-field gel electrophoresis was used to compare and analyze the genetic relationship among 40 Escherichia coli isolates obtained from local beef, Australia beef and India beef.
Ang, Chung Huap.
core  

Quantitative analysis of DNA‐GATA1 binding alterations linked to hematopoietic disorders

The FEBS Journal, EarlyView.
Native holdup allows the quantitative determination of affinities between full‐length transcription factors and DNA. Mutations in either the protein or the DNA can modulate binding strength, which can be precisely quantified using this approach. Applied to GATA1, it revealed mutations that alter DNA binding.
Boglarka Zambo, Bastien Morlet, Luc Negroni, Orsolya Mózner, Balázs Sarkadi, Gilles Travé, Gergo Gogl   +6 more
wiley   +1 more source

Mechanistic insights into HapC: A key enzyme in prodiginine biosynthesis in Hahella chejuensis

The FEBS Journal, EarlyView.
HapC catalyzes prodiginine biosynthesis through coordinated movements of its ATP‐binding, substrate‐binding, and catalytic‐swivel domains. The enzyme shows tolerance toward short‐chain MAP analogs, and docking analyses reveal key residues and structural features underlying its substrate flexibility.
Yueh‐Te Chan, Aden Dhana Rizkita, Hsin‐Ju Hsieh, Shu‐Fang Hsu, Tony Eight Lin, Wei‐Jan Huang, Kai‐Cheng Hsu, Sheila Nathan, Peiching Chang, Andrew H.‐J. Wang, Cheng‐Chung Lee   +10 more
wiley   +1 more source