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Pulsed-field gel electrophoresis
Molecular Biotechnology, 1998Pulsed-field gel electrophoresis (PFGE) was originally developed as a technique for providing electrophoretic karyotypes of micro-organisms. Since then the technique has evolved and diversified in many new directions. This review traces the evolution of PFGE, summarizes our understanding of its theoretical basis, and provides a comprehensive ...
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Pulsed-field gel electrophoresis
1992In view of the great impact of restriction fragment length polymorphism (RFLP) markers on both basic and applied plant research, considerable effort is currently being spent in constructing complete genetic linkage maps of the genomes of a wide variety of plant species.
Raymond Van Daelen, Pim Zabel
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Pulsed-field gel electrophoresis.
Methods in enzymology, 1996Campylobacter is a common foodborne pathogen. In most outbreaks, the source of infection is not found, due to the time passing from infection to diagnosis. In processed foods, Campylobacter may also become stressed and difficult to culture. In milkborne outbreaks, the contaminated milk is usually not kept long enough, and the bulk milk is not ...
J C, Wrestler +3 more
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Pulsed‐Field Gel Electrophoresis
Current Protocols in Molecular Biology, 2000AbstractDNA molecules longer than 25 kb are poorly resolved by standard agarose gel electrophoresis. These longer molecules can be resolved using several techniques that periodically change the direction of the electric field in the gel. This unit describes the simplest and most generally useful of the pulsed‐field techniques, field inversion ...
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Pulse Field Gel Electrophoresis
2015International ...
Jordan, Kieran, Dalmasso, Marion
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Pulsed-Field Gel Electrophoresis
Genome, 1989The introduction of pulsed — field — gel techniques for separating large DNA molecules has had an invigorating effect on the study of chromosomal DNA molecules, genome structure and electrophoretic theory. Indeed, the story of pulsed — field gels--like those of the Southern experiment or the polymerase-chain reaction--is a good illustration of the ...
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Methods for pulsed-field gel electrophoresis
Applied Biochemistry and Biotechnology, 1993The term pulsed-field gel electrophoresis (PFGE) is used as an acronym to indicate any technique that resolves (large) DNA molecules by continuous reorientation. It bridges the resolution gap between cytogenetic methods (> 5 Mb) and DNA analysis (< 50 kb).
J T, Den Dunnen, G J, van Ommen
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Pulsed Field Gel Electrophoresis for Bifidobacterium
2015Pulse Field Gel Electrophoresis (PFGE), unlike conventional electrophoresis, can resolve DNA fragments greater than 30 kb and is a highly discriminatory molecular typing method. Here we describe a PFGE protocol for bifidobacteria characterized by a short lysis time determined by the addition of lysis reagents to the initial cell suspension, a reduced ...
Esther, Jiménez +2 more
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Pulsed Field Gel Electrophoresis for Mycobacteria
2003Reference EPFL-CHAPTER-151283doi:10.1385/0-89603-471-2:51View record in PubMed Record created on 2010-09-07, modified on 2017-05 ...
W J, Philipp +3 more
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Pulsed Field Gel Electrophoresis
1995Abstract Pulsed field gel electrophoresis (PFGE) is the most reliable method for separating large fragments of DNA and has had a significant impact on the analysis of both prokaryotic and eukaryotic genomes. This book presents detailed chapters on both the theory behind PFGE and protocols for most of its major research and diagnostic ...
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